LOCUS       sequence-22986-e         319 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Schmelzle T, Mailleux AA, Overholtzer M, Carroll JS, Solimini NL, 
            Lightcap ES, Veiby OP, Brugge JS
  TITLE     Functional role and oncogene-regulated expression of the BH3-only 
            factor Bmf in mammary epithelial anoikis and morphogenesis.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Mar 6. 104(10):3787-92.
  PUBMED    17360431
REFERENCE   2  (bases 1 to 319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 Mar 6. 104(10):3787-92."
COMMENT     Sequence Label: pMKO shRNA Bmf
FEATURES             Location/Qualifiers
     source          1..319
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        59..307
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     59..79
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     230..249
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ccctcactcc ttctctaggc gccggaattg aagatctgtc gacaaggtcg ggcaggaaga
       61 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
      121 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
      181 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
      241 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
      301 aaacaccggt gctggctat
//
