LOCUS       sequence-229884-        7768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian expression of Amoeba myosin 1C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   Saydmohammed M, Yagi H, Calderon M, Clark MJ, Feinstein T, Sun M, 
            Stolz DB, Watkins SC, Amack JD, Lo CW, Tsang M
  TITLE     Vertebrate myosin 1d regulates left-right organizer morphogenesis 
            and laterality.
  JOURNAL   Nat Commun. 2018 Aug 23;9(1):3381. doi: 10.1038/s41467-018-05866-2.
  PUBMED    30139971
REFERENCE   2  (bases 1 to 7768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-08-23"; volume: "9(1):3381. doi"; pages: " 
            10.1038/s41467-018-05866-2"
COMMENT     Sequence Label: pCS2+ AMIC
FEATURES             Location/Qualifiers
     source          1..7768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             93..119
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3820..3954
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3844..3863)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3898..3917
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4067..4087)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4067..4085)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4106..4122)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4106..4122)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4119..4141)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4130..4146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4154..4160,4161..4178,4179..4184))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4199..4220
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4337..4354)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4508..5096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4588..4607)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5267..6058,6059..6127))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5890..5909
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6128..6232)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6258..6713)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6395..6416)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6607..6626
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6784..7768
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcgccgcca tgtacccata cgacgtccca gactacgctg
      121 gaggatccat ggcgtatacg agcaagcatg gcgtcgacga catggtgatg ttgacgagca
      181 tctccaacga cgccatcaac gacaacctca agaagcgctt cgccgccgac ctcatctaca
      241 cgtacatcgg tcacgtgctc atctcggtca acccctacaa acaaatcaac aacctctaca
      301 cagaacgtac gttgaaggac taccgcggca agtaccgtta tgagctgccc ccgcacgtct
      361 acgccctggc cgacgacatg taccgcacca tgctctccga gagcgaggac caatgcgtca
      421 tcatttctgg cgagtcggga gccggtaaga ccgaggcgtc gaagaagatc atgcagtaca
      481 tcgccgccgt ttcgggtgcc acgggcgacg tgatgcgcgt gaaggacgtc atcctcgagt
      541 ccaacccgct gctcgaggcc ttcggtaacg ccaagaccat caggaacaac aactcctctc
      601 gcttcggtaa gtacatggag atccagttcg acttgaaggg cgaccccgtg ggcggccgta
      661 tctccaacta cctgctcgag aagtcgcgcg tcgtgtacca gacgaatggc gagaggaact
      721 tccacatctt ctaccagctc ctggccggcg cgcccgccga cctgaggcag gaattcgggt
      781 tgcagacccc ggactactat ttctacctca accagggcaa gacctacacc gtcgacggca
      841 tggacgacaa ccaggaattc caagacacct ggaacgcgat gaaggtgatc ggattcacgg
      901 cggaggagca gcacgagatc ttccgtctcg tgacggccat cctctacctg ggcaacgtcc
      961 agttcgtgga cgacggcaag ggcggctcca ccatcgccga caggcaggtt gtggagatgc
     1021 tggcctatct catgaggacc gagccggtgg ccgtggagca ggccctgctg taccgcacca
     1081 tcaccaccgg cgagcagggc cgcggccgct cgtccgtcta ctcctgcccg caggaccccc
     1141 tcggcgccat ctactcgagg gacgccctgt cgaaggccct ctactcgcgc atgttcgact
     1201 acatcatcca gagagtcaac gacgccatgt acatcgacga ccccgaggcc ctcaccaccg
     1261 gtattctcga catctacggt tttgagatct tcggtaaaaa cggattcgag cagctgtgca
     1321 tcaacttcgt gaacgagaag ctgcagcaga tcttcatcca gctcacgctc aaggccgagc
     1381 aggaggagta cggcgccgag ggcatccagt gggagaacat cgactacttc aacaacaaga
     1441 tttgctgcga tctcatcgag gagaagcgtc ctccgggtct gatgaccatc ctcgacgacg
     1501 tgtgcaactt ccccaagggc actgacgaca agttccgcga gaagctcctg ggcgccttcc
     1561 cgacccacgc ccacttggcg gccacgtccc agcctgacga gttcgtcatc aagcactacg
     1621 ccggcgacgt ggtctacaac gtcgacggct tctgcgacaa gaacaaggat ctgctcttca
     1681 aggatctcat cggtctcgcc gagtgcacct cttccacctt cttcgccggt ctcttccccg
     1741 aggccaagga ggtcgccacc tccaagaaga agcccaccac cgccggcttc aagatcaagg
     1801 agtcgatcaa cattctggtg gccaccctgt ccaagtgcac cccgcactac atccgttgca
     1861 tcaagcccaa cgagaagaag gcggccaacg acttcaacaa ctcgctggtg ctccatcaag
     1921 tcaagtacct gggtctgctc gagaacgtgc gcatccgtcg cgccggttac gcctaccgtc
     1981 aggtctacga caagttcttc taccgctacc gcgtcgtgtg ccccaagacc tggtccggct
     2041 ggaacggcga catggtctcc ggcgctgagg cgatcctgaa ccacgtgggc atgtccctgg
     2101 gcaaggagta ccagaagggt aagaccaaga tcttcatccg ccagcccgag agcgtgttct
     2161 cgctcgagga gctgcgcgac cgcaccgtgt tctcctacgc caacaagatc cagagattcc
     2221 tccgcaagac cgccatgagg aagtactact acgaggtcaa gaagggcggc aatgacgcgc
     2281 tcgtcaacaa gaaggagcgt cgtcgcctgt cgctcgagcg tccgttcaag accgactaca
     2341 tcaactaccg ccagaacttc aagctcaagg actgcatcgg cgacaagggt acggagaagg
     2401 tgctgttcgc ggacctgtgc aacaacctgg acaagagctt ctggggctcg aaggtggagc
     2461 ggcgcatcat ggtgctcacc tccaacgcca tgttcctggt ggccatcgac cccaacaagg
     2521 acaagatcga gaagaaggtc aagcccttcc tctacgtcct caagcgccgc atcgacttca
     2581 acaagatcgg ctccatcacc ctcagcccgc tgcaggacaa cttcatgctg atcagcgtga
     2641 acggggagca cagcaacctg ctcgagtgcc gcaggaagac cgagctcatc ggcgtcctcc
     2701 tcaagcacaa cccgtccgtc cgcatccagt tcgccgacac gttcaacgtg acgctcaagg
     2761 gcggcaagac gtgcgtggtc aagttcatcc gcgacccgca gggcggcgac ggcaaggtca
     2821 agggcaccaa ggtctccgtc gcgcccggcc ttcccccctc ctccgcgccc aacatccagg
     2881 cgccgcagga gacgtcgggc ggtgcgtcgt tcacggtggc cgagcagtcc tacaaggacc
     2941 agatcctcgg cgccaaggga ggcggcggcg gcggcggaag gggcagaggc ggaccctcgc
     3001 ccagcggcgc cgtttcgccc cggccctcgc ccggcggtgg cggcggcggc ccgtccccgt
     3061 tcggcggccg tccctcgcct tcgggtcccc ccgccgccgc gtccgcgccc ggacccgagc
     3121 aggcgcgtgc gctgtatgac tttgcggcgg agaacccgga cgagctgacc ttcaacgagg
     3181 gcgccgtggt gacggtgatc aacaagagca acccggactg gtgggagggc gaactcaacg
     3241 gccagcgcgg cgtcttcccc gcgtcctacg tcgaactcat cccgcgcgcc gctgcgcccg
     3301 cgcccggccc ctccggcggc cctcgccccg ctccccccgg agctggaagg gcggcgccga
     3361 tgggcggacc cggtccgatg aggggccgag gcggacccgc gcctggcggt cccggcaggg
     3421 gcggtgcgcc cccgcccggc gccggcaggg ccggtccccc cggcggccgc ggcatgcccg
     3481 cgcccggcgg cgctgccccg cgcggcaggg gcgcgcctcc tcccggcgct ggcggccctc
     3541 ccggcggcgg ccgaggcgga gctccgccgc ctggtggtat gaggggtcgt ggtggtcctg
     3601 gtccggcgcc gccgggcggc atggcgagag gaggcatgat gccccctcgc ggcagggcgg
     3661 gtcctcctcc ccccggcatg taagtaggcc ggaccaatgc agcgttgtta cgtcgacgtg
     3721 gcaagaacgg caacccgtgt gcacatcaag ctcgcggatc cgagctcgag atctgcagct
     3781 ggtacccggg tctagaacta tagtgagtcg tattacgtag atccagacat gataagatac
     3841 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     3901 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     3961 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaattc
     4021 gcggccgcgg cgccaatgca ttgggcccgg tacccagctt ttgttccctt tagtgagggt
     4081 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4141 tcacaattcc acacaacata cgagccggga gcataaagtg taaagcctgg ggtgcctaat
     4201 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     4261 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4321 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     4381 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4441 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4501 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4561 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4621 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4681 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4741 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4801 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4861 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4921 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     4981 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5041 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5101 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5161 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     5221 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     5281 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     5341 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     5401 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     5461 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     5521 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     5581 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     5641 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5701 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     5761 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5821 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5881 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5941 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6001 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6061 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6121 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6181 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6241 cccgaaaagt gccacctaaa ttgtaatcgt taatattttg ttaaaattcg cgttaaattt
     6301 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     6361 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     6421 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     6481 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     6541 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     6601 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     6661 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     6721 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     6781 ccagtcgacc gccaattcaa tatggcgtat atggactcat gccaattcaa tatggtggat
     6841 ctggacctgt gccaattcaa tatggcgtat atggactcgt gccaattcaa tatggtggat
     6901 ctggacccca gccaattcaa tatggcggac ttggcaccat gccaattcaa tatggcggac
     6961 ctggcactgt gccaactggg gaggggtcta cttggcacgg tgccaagttt gaggaggggt
     7021 cttggccctg tgccaagtcc gccatattga attggcatgg tgccaataat ggcggccata
     7081 ttggctatat gccaggatca atatataggc aatatccaat atggccctat gccaatatgg
     7141 ctattggcca ggttcaatac tatgtattgg ccctatgcca tatagtattc catatatggg
     7201 ttttcctatt gacgtagata gcccctccca atgggcggtc ccatatacca tatatggggc
     7261 ttcctaatac cgcccatagc cactccccca ttgacgtcaa tggtctctat atatggtctt
     7321 tcctattgac gtcatatggg cggtcctatt gacgtatatg gcgcctcccc cattgacgtc
     7381 aattacggta aatggcccgc ctggctcaat gcccattgac gtcaatagga ccacccacca
     7441 ttgacgtcaa tgggatggct cattgcccat tcatatccgt tctcacgccc cctattgacg
     7501 tcaatgacgg taaatggccc acttggcagt acatcaatat ctattaatag taacttggca
     7561 agtacattac tattggaagt acgccagggt acattggcag tactcccatt gacgtcaatg
     7621 gcggtaaatg gcccgcgatg gctgccaagt acatccccat tgacgtcaat ggggaggggc
     7681 aatgacgcaa atgggcgttc cattgacgta aatgggcggt aggcgtgcct aatgggaggt
     7741 ctatataagc aatgctcgtt tagggaac
//
