LOCUS       sequence-229891-       11799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generation of unmarked gene deletions in Mycobacterium smegmatis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11799)
  AUTHORS   Shenkerman Y, Elharar Y, Vishkautzan M, Gur E
  TITLE     Efficient and simple generation of unmarked gene deletions in 
            Mycobacterium smegmatis.
  JOURNAL   Gene. 2014 Jan 1;533(1):374-8. doi: 10.1016/j.gene.2013.09.082. Epub
            2013 Oct 4.
  PUBMED    24100088
REFERENCE   2  (bases 1 to 11799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.gene.2013.09.082"; journalName: "Gene"; date: "2014-01-1-
            1"; volume: "533"; issue: "1"; pages: "374-8"
COMMENT     Sequence Label: pYS1
FEATURES             Location/Qualifiers
     source          1..11799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        214..646
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(647..733,734..2068)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     rep_origin      complement(2235..2823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2315..2334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5967..6782)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6690..6709
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(6943..6989)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggtaccgata tctgatttag gatacatttc tagccacctg acgtcggggg gggggggaaa
       61 gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
      121 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
      181 aacgggaaac gtcttgctcg accatatacc tgccgttcac tattatttag tgaaatgaga
      241 tattatgata ttttctgaat tgtgattaaa aaggcaactt tatgcccatg caacagaaac
      301 tataaaaaat acagagaatg aaaagaaaca gatagatttt ttagttcttt aggcccgtag
      361 tctgcaaatc cttttatgat tttctatcaa acaaaagagg aaaatagacc agttgcaatc
      421 caaacgagag tctaatagaa tgaggtcgaa aagtaaatcg cgcgggtttg ttactgataa
      481 agcaggcaag acctaaaatg tgtaaagggc aaagtgtata ctttggcgtc accccttaca
      541 tattttaggt ctttttttat tgtgcgtaac taacttgcca tcttcaaaca ggagggctgg
      601 aagaagcaga ccgctaacac agtacataaa aaaggagaca tgaacgatga acatcaaaaa
      661 gtttgcaaaa caagcaacag tattaacctt tactaccgca ctgctggcag gaggcgcaac
      721 tcaagcgttt gcgaaagaaa cgaaccaaaa gccatataag gaaacatacg gcatttccca
      781 tattacacgc catgatatgc tgcaaatccc tgaacagcaa aaaaatgaaa aatatcaagt
      841 tcctgaattc gattcgtcca caattaaaaa tatctcttct gcaaaaggcc tggacgtttg
      901 ggacagctgg ccattacaaa acgctgacgg cactgtcgca aactatcacg gctaccacat
      961 cgtctttgca ttagccggag atcctaaaaa tgcggatgac acatcgattt acatgttcta
     1021 tcaaaaagtc ggcgaaactt ctattgacag ctggaaaaac gctggccgcg tctttaaaga
     1081 cagcgacaaa ttcgatgcaa atgattctat cctaaaagac caaacacaag aatggtcagg
     1141 ttcagccaca tttacatctg acggaaaaat ccgtttattc tacactgatt tctccggtaa
     1201 acattacggc aaacaaacac tgacaactgc acaagttaac gtatcagcat cagacagctc
     1261 tttgaacatc aacggtgtag aggattataa atcaatcttt gacggtgacg gaaaaacgta
     1321 tcaaaatgta cagcagttca tcgatgaagg caactacagc tcaggcgaca accatacgct
     1381 gagagatcct cactacgtag aagataaagg ccacaaatac ttagtatttg aagcaaacac
     1441 tggaactgaa gatggctacc aaggcgaaga atctttattt aacaaagcat actatggcaa
     1501 aagcacatca ttcttccgtc aagaaagtca aaaacttctg caaagcgata aaaaacgcac
     1561 ggctgagtta gcaaacggcg ctctcggtat gattgagcta aacgatgatt acacactgaa
     1621 aaaagtgatg aaaccgctga ttgcatctaa cacagtaaca gatgaaattg aacgcgcgaa
     1681 cgtctttaaa atgaacggca aatggtacct gttcactgac tcccgcggat caaaaatgac
     1741 gattgacggc attacgtcta acgatattta catgcttggt tatgtttcta attctttaac
     1801 tggcccatac aagccgctga acaaaactgg ccttgtgtta aaaatggatc ttgatcctaa
     1861 cgatgtaacc tttacttact cacacttcgc tgtacctcaa gcgaaaggaa acaatgtcgt
     1921 gattacaagc tatatgacaa acagaggatt ctacgcagac aaacaatcaa cgtttgcgcc
     1981 aagcttcctg ctgaacatca aaggcaagaa aacatctgtt gtcaaagaca gcatccttga
     2041 acaaggacaa ttaacagtta acaaataagc ggccgctcgc tgcgctcggt cgttcggctg
     2101 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2161 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2221 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2281 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2341 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2401 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2461 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2521 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2581 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2641 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     2701 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2761 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2821 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga gtatcgagcc
     2881 gagaacgtta tcgaagttag tcatgtgtaa tcccctcgtt tgaactttgg attaagcgta
     2941 gatacaccct tggacaagcc agttggattc ggagacaagc aaattcagcc ttaaaaaggg
     3001 cgaggccctg cggtggtgga acaccgcagg gcctctaacc gctcgacgcg ctgcaccaac
     3061 cagcccgcga acggctggca gccagcgtaa ggcgcggctc atcgggcggc gttcgccacg
     3121 atgtcctgca cttcgagcca agcctcgaac acctgctggt gtgcacgact cacccggttg
     3181 ttgacaccgc gcgcggccgt gcgggctcgg tggggcggct ctgtcgccct tgccagcgtg
     3241 agtagcgcgt acctcacctc gcccaacagg tcgcacacag ccgattcgta cgccataaag
     3301 ccaggtgagc ccaccagctc cgtaagttcg ggcgctgtgt ggctcgtacc cgcgcattca
     3361 ggcggcaggg ggtctaacgg gtctaaggcg gcgtgtacgg ccgccacagc ggctctcagc
     3421 ggcccggaaa cgtcctcgaa acgacgcatg tgttcctcct ggttggtaca ggtggttggg
     3481 ggtgctcggc tgtcgctggt gttccaccac cagggctcga cgggagagcg ggggagtgtg
     3541 cagttgtggg gtggcccctc agcgaaatat ctgacttgga gctcgtgtcg gaccatacac
     3601 cggtgattaa tcgtggtcta ctaccaagcg tgagccacgt cgccgacgaa tttgagcagc
     3661 tctggctgcc gtactggccg ctggcaagcg acgatctgct cgaggggatc taccgccaaa
     3721 gccgcgcgtc ggccctaggc cgccggtaca tcgaggcgaa cccaacagcg ctggcaaacc
     3781 tgctggtcgt ggacgtagac catccagacg cagcgctccg agcgctcagc gcccgggggt
     3841 cccatccgct gcccaacgcg atcgtgggca atcgcgccaa cggccacgca cacgcagtgt
     3901 gggcactcaa cgcccctgtt ccacgcaccg aatacgcgcg gcgtaagccg ctcgcataca
     3961 tggcggcgtg cgccgaaggc cttcggcgcg ccgtcgacgg cgaccgcagt tactcaggcc
     4021 tcatgaccaa aaaccccggc cacatcgcct gggaaacgga atggctccac tcagatctct
     4081 acacactcag ccacatcgag gccgagctcg gcgcgaacat gccaccgccg cgctggcgtc
     4141 agcagaccac gtacaaagcg gctccgacgc cgctagggcg gaattgcgca ctgttcgatt
     4201 ccgtcaggtt gtgggcctat cgtcccgccc tcatgcggat ctacctaccg acccggaacg
     4261 tggacggact cggccgcgcg atctatgccg agtgccacac gcgaaacgcc gaattctcgt
     4321 gcaacgacgt gtgtcccgga ccgctaccgg acagcgaggt ccgcgccatc gccaacagca
     4381 tttggcgttg gatcacaacc aagtcgcgca tttgggcgga cgggatcgtg gtctacgagg
     4441 ccacactcag tgcgcgccag tcggccatct cgcggaaggg cgcagcagcg cgcacggcgg
     4501 cgagcacagt tgcgcggcgc gcaaagtccg cgtcagccat ggaggcattg ctatgagcga
     4561 cggctacagc gacggctaca accggcagcc gactgtccgc aaaaagcggc gcgtgaccgc
     4621 caccgaaggc gctcgaatca ccggactatc cgaacgccac gtcgtccggc tcgtggcgca
     4681 ggaacgcagc gagtggctcg ccgagcaggc tgcacgccgc gaacgcatcc gcgcctatca
     4741 cgacgacgag ggccactctt ggctgcaaac ggccaaacat ttcgggctgc atctggacac
     4801 cgttaagcga ctcggctatc gggcaaggaa agagcgtgcg gcagaacagg aagcggctca
     4861 aaaggcccac aacgaagccg acaatccacc gctgttctaa cgcaattggg gagcgggtgt
     4921 cgcgggggtt ccgtgggggg ttccgttgca acgggtcgga caggtaaaag tcctggtaga
     4981 cgctagtttt ctggtttggg ccatgcctgt ctcgttgcgt gtttcgttgc gcccgttttg
     5041 aataccagcc agacgagacg gggttctacg aatcttggtc gataccaagc catttccgct
     5101 gaatatcggg gagctcaccg ccagaatcgg tggttgtggt gatgtacgtg gcgaactccg
     5161 ttgtagtgcc tgtggtggca tccgtggcca ctctcgttgc acggttcgtt gtgccgttac
     5221 aggccccgtt gacagctcac cgaacgtagt taaaacatgc tggtcaaact aggtttacca
     5281 acgatacgag tcagctcatc tagggccagt tctaggcgtt gttcgttgcg cggttcgttg
     5341 cgcatgtttc gtgtggttgc tagatggctc cgcaaccaca cgcttcgagg ttgagtgctt
     5401 ccagcacggg cgcgatccag aagaacttcg tcgtgcgact gtcctcgtta acgctcgaag
     5461 cgatgccgat caaatcggct gtgccgtcgc ggttcttgcg gtacagcggc gcgcctgagt
     5521 cgccatgtac ggtgcgcagc tgagcggtga tcgtgtactt ggcagccaca acgaccggcc
     5581 cacaggtctc gccggatttc atcccgtact tgcagacctc ggtgccgtac ggaacatctt
     5641 tgcggtcctt cagcaccgca cgcacggggt atcttccgcc gattttgaac gaagcgatcg
     5701 gtgttcccgg agcaaacgca atcaccccag cgtcggtgta gaccggcaat agatcatcgt
     5761 cgccggtaat cgtggtgcct tcgtctctcg acgcaacgta ggtacccaac gtccgctgtg
     5821 tatccggttt ctctgcaacc gaatacagca ccgggccccc cctcgagttt aaacattttt
     5881 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccatgcatgt gctagcccag
     5941 tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaattgc
     6001 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
     6061 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     6121 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     6181 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag attatgcatt
     6241 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
     6301 accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta
     6361 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     6421 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg
     6481 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     6541 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
     6601 acgctacctt tgccatgttt cagaaacaac tctggcgcat ggggcttccc atacaagcga
     6661 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     6721 gcatccatgt tggaatttaa tcgcggcctc gagcaagacg tttcccgttg aatatggctc
     6781 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata
     6841 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttcccccccc
     6901 cccgacgtca ggtggctagc tgatcaccgc ggccatgatg gcataaaacg aaaggcccag
     6961 tctttcgact gagcctttcg ttttatttga tgcctggcag tcgatcgtac gctagttgca
     7021 gatcaaccac cgcgtcggaa ttcgccgggg cgctcatgcg ttgggcccgt cgaacatgtc
     7081 gatgacagcc ttcgcctcgt cgaacgtgag gtcggcggcg cgcgtggctt tgacgccggc
     7141 cgaatcggcg aggaacttga accaatcggc gtcgttgtac ttttcggcct tctggatctc
     7201 gccgaggcgc ttcagctgat cgggattggc catctgcacg tcgggcgccg gcgcggtggc
     7261 gcgctgcgcg cgttgtgcgc gcgacgactt cggtggcgta tcgacctcgt gcggctcggc
     7321 gatctcgccg tcgatgtagt cggccgtcgg cttgacggtc gacggcagcg cgagcgcctg
     7381 cgactgtgac aggtcggtgc ccgggcgatc gtcggcgcgg atcgccgcgt cgagccgcgt
     7441 cgtcttcggt gccagcttca gcacctgttt gagcgcggtc ttccgctcca tccagcgctg
     7501 cgggtccttg atgtcgcccg aggatccgac cttgccgcga cgcaattccc tgatctcgtc
     7561 ggcggtgaac acgtcccaca gcggctcagc gccggtcacc tcgacgatcg cgtagaagta
     7621 gaccggatta ccccgatcac ccttggcctt cacgtgtttc agcgtcggat tgaggcccat
     7681 ggtgtaatgg aattcgtcgt tcgcgccgac ccactgcgcg tcgatccggg aggcgcgcgg
     7741 gtgctgccag aacagtttga cgatgccctg gtagccgatg atcagctggc actcgaccac
     7801 gccgcgccgg gtgtcgcggt agggcacgag gtagcactcg ccgttgacac cgggctcgag
     7861 accgagcgcg gccgaggtca gcagcgtacc ggcaaatgac tcttgcgtgc agtcgtcgag
     7921 ggactgctta gcgatgccag cagctttcgc gttgcgcgac ttgcggatct cggtttgcac
     7981 gatccgcgcg atgcggtcgg ggtccatacc cttgggcact gcacgcgcca gctgcggcac
     8041 gagcacctgc agcacttgcg agatcgtctc gggtgccttg ggcgaatcct gcttggtgac
     8101 agcattttca gccatagatg taatcccctt caatcgggtc ggcctcttcg agcgggtcgg
     8161 tgtcgtagat atagctgtcg gtgacgacgt cgccgacggt ctgcggtcgc ggcagccccc
     8221 acgagggcag gctgatcgtg accacgtcgt cgctgtagtc aggccacttg cccgattcca
     8281 tgcagtcggc gtagagccgg atcgcctggc ggttgcggcg tcgcccctct tcgatcgcct
     8341 cgtcgtcgta ctggatcgga gtcacgacgt agggcggttc tttctcctgc gtgatgaacc
     8401 ggaatcgcgg gttctcggcg agcccgaggg cgaccagtag atcgatgtac caggccgcct
     8461 gcatgaaata gccgagcttg tagaacttgg ttttcagctc ggccgggttc gcggtcatcg
     8521 acgtcttgta atcatcgata tcgtcagtga gccagtcgat ccggccacgc agccgcacgc
     8581 cggtctcggg gtcggtgtag tacagcgcga cctcggcctc gccgtcagga ttggcgaaga
     8641 tcgggccggc tgtcgggtgc tgacgcacct tttcggccat gtcgtacgcc ttcgtgaaca
     8701 ggtcgacgtg aatcggcacc ttgccctgtt tgcgagcctc agcctcggcc ttgcgccaca
     8761 tgcccgtcgc ggtcggcttc tccgacggcg taccgtccgc cttcagcccg tgcacctcgg
     8821 ggtcgaggat ctcgaactcg gcacccttgc cgagcaccag tttgtgcgcg acatgtccga
     8881 agtcccagac ctttttcggc ttccgggtgt tgtccatctc ccagcggaat ttcgcgggac
     8941 acgacggcgg caacagcagc ttcgcgccgg ataccgacag cgagccccgg tcagcgtggt
     9001 agacgtcctc gtcgacgaac cggtgcattc cgtcctgtgt gggcacactc atatgcggcc
     9061 gccgatctcg taactacgtc gacatcgata agcttcgaat tctgcagctg gatccgttac
     9121 atatgtggac tccctttctc ttatcgggtg gtggccgcga gcgcggccgg tggagggttg
     9181 gtgactgacg gcgctgcgag acggcgcccg atggggtatg cgagaacaac tgcggcaaaa
     9241 ccgatcacgg cgatgaccgc ggcggggagc gggccggttt cccagttgcc cgacacgatc
     9301 aggaaggccg gcacggcggc ggtgatcacg ccttcggcca ccgcgacgaa cccgacggcg
     9361 ggccccagtg cgtcgtgccc caggccgagc agcaggaaca gcatgaacca cagcactgcc
     9421 cacagcagcc agatcacccc gaacgccggg tcggcctcgg cggtgaacgc gtgccacgag
     9481 tagccgagtg cggcgatcgc gacgaacagc gagaaccatc cgagaccttc tccgtcccag
     9541 tcggtcacgt tgttgatggc cacccacagg taggtgaagc cgaacaggta gagcccggag
     9601 gccgcgaaga tcacggccgc gtctccgccg gactgcagga tcagcaccgt aggcgtcacc
     9661 acctgcagtc cgccgacgaa gaagttcagc ggtgttgcgc ctcgtgggct gatgcggccc
     9721 agcagcatca gcccgtcgat gatgagcacc gcacccacgt agaagagccc gacgccaccc
     9781 atgtcgctag tacctcggca gactcggtag accgggattg cggcgcgggg cccgcgggcg
     9841 gtccgcgcct gcgggaatcg acttcaccac cgtgggactt tcggcgctcg cggacgccgc
     9901 gtcgaatgcg cggtgatgtc ccgcggtgaa tgtcgtcagc cctaccgaac cgaagacccg
     9961 gcgcgccggc gtccggcact ccggacaggt cgccgccgca tcgcgctcgg agatcgcgca
    10021 ggtgagatcg aagggaccgc agtgggaaca acggaatgtg taggtcggca tggtcacccc
    10081 tttccattca cccgaaatat atccagatga aaataaaagt caagggtgaa cgcccgcgcc
    10141 gggcgcgggt ggttacgaag tcaaattcga ggtggtgtcg acgagttcgc gcaactggtc
    10201 gacgtccagc ccgcacgatt cgaaagccgc acactcggcc tcggctaccg cgtccaccag
    10261 gcggttgcgc agcgtccgcc cccgcggggt caacgccacg agcacccgcc ggcggtcatg
    10321 cagatcggca gtccggtaga tgatcccttg cgacacaagg cgatcgacgg ttctggtgag
    10381 agtcggaccc gtgatcagcg tggcctgcgc gagatcgctc atgggcacgg tgcgccgtcc
    10441 ggccaactcg tcgagcacgc gccagtcctc gatcttgagc gacgagtccg cgagcacgcg
    10501 ttcgatcgcc gactccgcga ggtgggtcag acgcaccaac ggcagcagga ggttggggcg
    10561 gcgctgctgt ccggcggcca tggtgggtgt ctcctcgtgt cgacggcggg gtctgttcag
    10621 aatgaattat ttctgctaga aagctttgaa gcgcagacta cacgggatcg acggtgatct
    10681 ggagtgcagg acggcgaggt tgagttccgc gtcgggctgg tgattccgct tcaagggcca
    10741 gcgggaatct tcgcgccttc gtgcgaggcc gtggccgagc tcgcggccaa ggaggtcaac
    10801 gaccgaggcg gcctgcaggg ccgcaaggtc accatcgagg tgctcgacgg cggagcgccc
    10861 ggcgacgacg tcgcccggac cgtcgccgac cggttgcgag gtcacggtct cgacgccgtg
    10921 accggctggc acatctcggc cgtgcgcaac cgcatctccc cggtggtccg cgaccgcatc
    10981 ccgtacgtct acacctcgtt gtacgagggc ggtgaacgca caccgggcgt gttctgcaca
    11041 ggcgagacac cgcagatcca gatcgcgccc gcgctcgcct ggctgcgcga ccacttcggc
    11101 atccggtcct ggtgcctggt cggcgatgac tacatctggc cgcgccgttc cgccgcggcc
    11161 gcccgcgcgt actgccgaga tctggacctc gagctcagac gggagatcta cgtcccgtac
    11221 ggcaccgacg atttccgcgc acctgtccgc aaggccatcg cgtcgggggc gcaggccgtg
    11281 ttgatgctgc tcgtcggcca ggacgccgtg ttgttcaacc gcgagttcgc gcgcgccggc
    11341 gggcacgacc gcatggcgcg gttcagcccg ctgatggagg agaacatgct gctggccagc
    11401 ggcgccggct ccaccgaaaa cctctatgtc gccgcggcgt acttcagttc actggccacc
    11461 gcgggtgcca tggacctgat gggcagctac gtcgcccgct acggcgccga cgcgccaccg
    11521 ctcaacgcga tggccgaatc ctgttacgag ggtctgcttg cgctcgaggc catcttccag
    11581 cgggcccact cccccgagat accggacctg atggcatccg cgcacgatgt cgggttcgac
    11641 gggccccggg gcccgatgtg tatgcgcgac agtcaattcg accagcaggt ctacatcgcc
    11701 agcgccgacg gctacgactt cgacatcctc gacacgctgg cgacgctcga cgcttgagac
    11761 cgcgtcactt ctttatctag atttaaagat ctggtaccg
//
