LOCUS       sequence-229940-          82 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part for PlacO (common LacI-repressed 
            promoter; see DOI: 10.1126/science.1256272). Please see Supplemental
            Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 82)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 82)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P32m
FEATURES             Location/Qualifiers
     source          1..82
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..23
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    30..46
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggagaattgt gagcggataa caattgacat tgtgagcgga taacaagata ctgagcacat
       61 cagcaggacg cactgaccta ct
//
