LOCUS       sequence-22996-e         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Kohli M, Rago C, Lengauer C, Kinzler KW, Vogelstein B
  TITLE     Facile methods for generating human somatic cell gene knockouts 
            using recombinant adeno-associated viruses.
  JOURNAL   Nucleic Acids Res. 2004  . 32(1):e3.
  PUBMED    14704360
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2004  . 32(1):e3."
COMMENT     Sequence Label: pNeDaKO-Neo
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(101..120)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     711..730
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tctcttctat ctccgggcct ttcggcctgc agccaatatg ggatcggcca ttgaacaaga
       61 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
      121 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
      181 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
      241 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
      301 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
      361 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
      421 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
      481 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
      541 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
      601 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
      661 attcatcgac tgtggccggc tgggtgtggc ggatcgctat caggacatag cgttggctac
      721 ccgtgatatt gctgaagaac ttggcggcga atgggctgac ctctttg
//
