LOCUS       sequence-229960-          79 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part for pTac (Adam Meyer's improved 
            pTac promoter selected for dynamic range and low cross-sensitivity; 
            unpublished, using strategy from https://doi.org/10.1101/285866)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 79)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 79)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P61m
FEATURES             Location/Qualifiers
     source          1..79
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(22..27,28..43,44..50)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    56..75
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 ggagaacgat cgttggctgt gttgacaatt aatcatcggc tcgtataatg tgtggaattg
       61 tgagcgctca caatttact
//
