LOCUS       sequence-229970-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for deCFP (cyan fluorescent 
            protein) - Please see Supplemental Documents for annotated Genbank 
            file.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C9m
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(32..53)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(293..312)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     640..661
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aatggagctt ttcactggcg ttgttcccat cctggtcgag ctggacggcg acgtaaacgg
       61 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      121 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      181 gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
      241 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      301 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      361 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      421 ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
      481 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
      541 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
      601 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
      661 gaccgccgcc gggatctaaa ggt
//
