LOCUS       sequence-229976-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part with sfYFP-ssrA (superfolder YFP 
            with ssrA degradation tag; variant of 
            https://www.fpbase.org/protein/yfp/).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C20m
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..718
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 aatgatgcgt aaaggcgaag agctgttcac tggtgtcgtc cctattctgg tggaactgga
       61 tggtgatgtc aacggtcata agttttccgt gcgtggcgag ggtgaaggtg acgcaactaa
      121 tggtaaactg acgctgaagt tcatctgtac tactggtaaa ctgccggtac cttggccgac
      181 tctggtaacg acgctgactt atggtgttca gtgctttgct cgttatccgg accatatgaa
      241 gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac gcacgatttc
      301 ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag gcgataccct
      361 ggtaaaccgc attgagctga aaggcattga ctttaaagaa gacggcaata tcctgggcca
      421 taagctggaa tacaatttta acagccacaa tgtttacatc accgccgata aacaaaaaaa
      481 tggcattaaa gcgaatttta aaattcgcca caacgtggag gatggcagcg tgcagctggc
      541 tgatcactac cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc cagacaatca
      601 ctatctgagc taccaaagcg ttctgtctaa agatccgaac gagaaacgcg atcatatggt
      661 tctgctggag ttcgtaaccg cagcgggcat cacgcatggt atggatgaac tgtacaaaac
      721 tagtgcagcg aacgacgaaa attacgccct tgcagcgtga aggt
//
