LOCUS       sequence-229982-         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for lambda cI (repressor of pL or
            pOR1-OR2; use C64m for temperature-sensitive version). Please see 
            Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C24m
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..715
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 aatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa
       61 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa
      121 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc
      181 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc
      241 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag
      301 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag
      361 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc
      421 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag
      481 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt
      541 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg
      601 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg
      661 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gctgaaggt
//
