LOCUS       sequence-229998-         314 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, 
            modified synonymously to remove BsaI cut site; ccdA/C79m is 
            antitoxin). Please see Supplemental Documents for annotated Genbank 
            file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 314)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C39m
FEATURES             Location/Qualifiers
     source          1..314
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..310
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     189..208
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aatgatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
       61 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
      121 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga
      181 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtgtccgtta tcggggaaga
      241 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
      301 gggaatataa aggt
//
