LOCUS       sequence-230012-        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for LacI (Lac repressor; 
            identical to C1d but without ssrA degradation tag). Please see 
            Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C63m
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..1093
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(30..49)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 aatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
       61 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      121 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      181 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      241 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
      301 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
      361 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
      421 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
      481 cagtattatt ttctcccatg aggacggtac gcgactgggc gtggagcatc tggtcgcatt
      541 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
      601 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
      661 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
      721 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
      781 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
      841 agatagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
      901 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
      961 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     1021 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1081 aagcgggcag taaaggt
//
