LOCUS       sequence-230014-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for cI857 (temperature-sensitive 
            lambda repressor that represses at 30C but not at 37C; see 
            https://doi.org/10.1016/S0022-2836(66)80269-3. Use with P9m)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C64m
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..718
                     /codon_start=1
                     /gene="cIts"
                     /product="temperature-sensitive variant of the phage lambda
                     repressor"
                     /label=lambda repressor (ts)
                     /note="thermosensitivity is conferred by the A67T mutation"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 aatgatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg cacgtcgcct
       61 taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat ctgtcgcaga
      121 caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca atgcattaaa
      181 tgcttataac gccgcattgc ttacaaaaat tctcaaagtt agcgttgaag aatttagccc
      241 ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc cgtcacttag
      301 aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct cacctaagct
      361 tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa aagccagtga
      421 ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc
      481 aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg agccaggtga
      541 tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga tcagggatag
      601 cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat gcaatgagag
      661 ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt ttggctgaag
      721 gt
//
