LOCUS       sequence-230016-        2279 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for Bxb1-mScarleti-LAA (Bxb1 
            integrase tagged with an mScarlet-I reporter and an LAA degradation 
            tag). Please see Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2279)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C67m
FEATURES             Location/Qualifiers
     source          1..2279
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..1504
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MVRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDL
                     DVSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKL
                     VVSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWG
                     YLPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQ
                     GREPQGREWSATALKRSMISGAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRA
                     ELVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGT
                     VAMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRA
                     GSPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRS
                     MNVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
ORIGIN
        1 aatggtgaga gccctggtag tcatccgcct gtcccgcgtc accgatgcta cgacttcacc
       61 ggagcgtcag ctggagtctt gccagcagct ctgcgcccag cgcggctggg acgtcgtcgg
      121 ggtagcggag gatctggacg tctccggggc ggtcgatccg ttcgaccgga agcgcagacc
      181 gaacctggcc cggtggctag cgttcgagga gcaaccgttt gacgtgatcg tggcgtaccg
      241 ggtagatcgg ttgacccgat cgatccggca tcttcagcag ctggtccact gggccgagga
      301 ccacaagaag ctggtcgtct ccgcgaccga agcgcacttc gatacgacga cgccgtttgc
      361 ggcggtcgtc atcgcgctta tgggaacggt ggcgcagatg gaattagaag cgatcaaaga
      421 gcggaaccgt tcggctgcgc atttcaatat ccgcgccggg aaataccgag gatccctgcc
      481 gccgtgggga tacctgccta cgcgcgtgga cggggagtgg cggctggtgc cggaccctgt
      541 gcagcgagag cgcatcctcg aggtgtatca ccgcgtcgtc gacaaccacg agccgctgca
      601 tctggtggcc cacgacctga accggcgtgg tgtcctgtcg ccgaaggact acttcgcgca
      661 gctgcaaggc cgcgagccgc agggccggga gtggtcggct accgcgctga agcgatcgat
      721 gatctccggg gcgatgctcg ggtacgcgac tctgaacggt aagaccgtcc gagacgacga
      781 cggagccccg ctggtgcggg ctgagccgat cctgacccgt gagcagctgg aggcgctgcg
      841 cgccgagctc gtcaagacct cccgggcgaa gcccgcggtg tctaccccgt cgctgctgct
      901 gcgggtgttg ttctgcgcgg tgtgcgggga gcccgcgtac aagttcgccg ggggaggacg
      961 taagcacccg cgctaccgct gccgctcgat ggggttcccg aagcactgcg ggaacggcac
     1021 ggtggcgatg gccgagtggg acgcgttctg cgaggagcag gtactggatc tgctcgggga
     1081 cgcggagcgt ctggagaaag tctgggtagc gggctcggac tccgcggtcg aactcgcgga
     1141 ggtgaacgcg gagctggtgg acctgacgtc gctgatcggc tccccggcct accgggcggg
     1201 ctctccgcag cgagaagcac tggatgcccg tattgcggcg ctggccgcgc ggcaagagga
     1261 gctggagggc ctggaggctc gcccgtctgg ctgggagtgg cgcgaaaccg ggcagcggtt
     1321 cggggactgg tggcgggagc aggacaccgc ggcaaagaac acctggcttc ggtcgatgaa
     1381 cgttcggctg acgttcgacg tccgcggcgg gctgactcgc acgatcgact tcggggatct
     1441 tcaggagtac gagcagcatc tcaggctcgg cagcgtggtc gaacggctac acaccgggat
     1501 gtcgggtagc ggcagcggta gcggtagtgg gagcggcagc atggtcagta aaggcgaagc
     1561 agttatcaaa gagttcatgc gcttcaaagt tcatatggaa gggtcgatga acgggcacga
     1621 atttgaaatt gaaggcgaag gcgaaggccg cccatatgaa gggacccaaa ccgcaaagct
     1681 taaggttact aaaggcggtc cattaccctt ttcgtgggac attttaagcc cacagtttat
     1741 gtacgggagt cgcgctttca tcaagcaccc tgcggacatc ccagattact acaaacagtc
     1801 tttccccgag gggttcaagt gggagcgcgt gatgaacttc gaggatggcg gagccgtgac
     1861 ggtcacccaa gatacctctt tggaggacgg tacgttgatc tacaaagtga aattgcgtgg
     1921 cacgaatttt ccacctgatg ggcctgtcat gcagaaaaag acaatgggat gggaagcttc
     1981 cacggagcgc ctttacccag aggacggtgt tcttaaaggg gatatcaaaa tggcgctgcg
     2041 tcttaaagat ggaggccgct acctggcgga cttcaagact acttacaagg ccaaaaaacc
     2101 agtgcagatg ccgggtgcgt acaatgtaga tcgtaaatta gatattacaa gtcacaatga
     2161 agattacacg gtcgtagagc agtatgagcg cagtgagggg cgtcactcta cgggcggtat
     2221 ggacgagtta tacaaggcgg ctaacgacga gaattatgca ttggccgcct aataaaggt
//
