LOCUS       sequence-230022-        1087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for LacI-MVY (Adam Meyer's 
            improved LacI repressor from https://doi.org/10.1101/285866, 
            selected for dynamic range and low cross-sensitivity)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1087)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C72m
FEATURES             Location/Qualifiers
     source          1..1087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..1084
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYMTVSRVVNQASHVSAKTREKVEAAMVEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIYSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(21..40)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 aatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atatgaccgt
       61 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
      121 ggcgatggtg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
      181 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt
      241 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga
      301 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag
      361 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg
      421 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
      481 ttactcccat gaggacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
      541 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg
      601 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
      661 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac
      721 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
      781 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagatagctc
      841 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
      901 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
      961 agtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
     1021 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     1081 gtgaggt
//
