LOCUS       sequence-230030-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for KanR (kanamycin resistance 
            protein). Please see Supplemental Documents for annotated Genbank 
            file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C76m
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..820
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(78..97)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aatgatgagc catattcaac gggaaacgtc ttgctcccgt ccgcgcttaa actccaacat
       61 ggacgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
      121 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
      181 tagcgttgcc aatgatgtta cagatgagat ggtccgtctc aactggctga cggagtttat
      241 gcctctcccg accatcaagc attttatccg tactcctgat gatgcgtggt tactcaccac
      301 cgcgattcct gggaaaacag ccttccaggt attagaagaa tatcctgatt caggtgaaaa
      361 tattgttgat gcgctggccg tgttcctgcg ccggttacat tcgattcctg tttgtaattg
      421 tccttttaac agcgatcgtg tatttcgtct tgctcaggcg caatcacgca tgaataacgg
      481 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
      541 gaaagaaatg cacaagctct tgccattctc accggattca gtcgtcactc atggtgattt
      601 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
      661 ggtcggaatc gcagaccgtt accaggacct tgccattctt tggaactgcc tcggtgagtt
      721 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
      781 taaattgcag tttcatttga tgctcgatga gtttttctaa aggt
//
