LOCUS       sequence-230032-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin
            resistance protein). Please see Supplemental Documents for annotated
            Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C77m
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(5..73,74..865)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(223..242)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aatgatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
       61 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      121 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      181 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      241 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      301 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      361 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      421 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      481 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      541 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      601 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      661 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      721 gtcgcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      781 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      841 tgcctcactg attaagcatt ggtaaaggt
//
