LOCUS       sequence-230034-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD cloning part for ChlorR 
            (chloramphenicol resistance protein). Please see Supplemental 
            Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C78m
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..664
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(71..90)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aatgatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
       61 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
      121 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
      181 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga
      241 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
      301 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
      361 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
      421 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
      481 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
      541 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
      601 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
      661 gtaaaggt
//
