LOCUS       sequence-23004-e         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Jallepalli PV, Waizenegger IC, Bunz F, Langer S, Speicher MR, Peters
            JM, Kinzler KW, Vogelstein B, Lengauer C
  TITLE     Securin is required for chromosomal stability in human cells.
  JOURNAL   Cell. 2001 May 18. 105(4):445-57.
  PUBMED    11371342
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2001 
            May 18. 105(4):445-57."
COMMENT     Sequence Label: pFredA
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    59..92
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        94..451
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        94..285
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(117..137)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      302..437
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(540..559)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gtggcggccg ctctagacta gtggatcccc cgggctgcag gaattcgata tcaagcttat
       61 aacttcgtat aatgtatgct atacgaagtt atcctgaggc ggaaagaacc agctgtggaa
      121 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      181 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
      241 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
      301 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
      361 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
      421 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
      481 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
      541 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
      601 tgtcagcgca ngggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
      661 aactgcaaga cgaggcagcg cggctat
//
