LOCUS       sequence-23009-e         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B
  TITLE     PUMA induces the rapid apoptosis of colorectal cancer cells.
  JOURNAL   Mol Cell. 2001 Mar . 7(3):673-82.
  PUBMED    11463391
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2001 Mar . 7(3):673-82."
COMMENT     Sequence Label: pHAHA
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             81..107
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     polyA_signal    137..271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(161..180)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     215..234
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(414..436)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 tagcagagct cgtttagtga ccgtcagatc tctagaagct gggtaccgaa atgggatacg
       61 atgttccaga ttacgcttcg tacgatgttc cagattacgc aagcttgcta gcggccgctc
      121 gaggccggca aggccggatc cagacatgat aagatacatt gatgagtttg gacaaaccac
      181 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
      241 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
      301 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
      361 tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
      421 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
      481 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gaggtcgact
      541 ctagaggatc gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc
      601 ttcgcggggc agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct
      661 gttccacatg tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca
      721 tccttataaa tggatgtgca ca
//
