LOCUS       sequence-230134-         110 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. 
            pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA 
            structure and open targeting region)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 110)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UC20m
FEATURES             Location/Qualifiers
     source          1..110
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        25..100
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tactctcctc ctccccccca atatgtttta gagctagaaa tagcaagtta aaataaggct
       61 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttaggt
//
