LOCUS       sequence-230148-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor for precise CRISPR directed genomic integration using the 
            GeneWeld method.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Wierson WA, Welker JM, Almeida MP, Mann CM, Webster DA, Torrie ME, 
            Weiss TJ, Kambakam S, Vollbrecht MK, Lan M, McKeighan KC, Levey J, 
            Ming Z, Wehmeier A, Mikelson CS, Haltom JA, Kwan KM, Chien CB, 
            Balciunas D, Ekker SC, Clark KJ, Webber BR, Moriarity BS, Solin SL, 
            Carlson DF, Dobbs DL, McGrail M, Essner J
  TITLE     Efficient targeted integration directed by short homology in 
            zebrafish and mammalian cells.
  JOURNAL   Elife. 2020 May 15;9. pii: 53968. doi: 10.7554/eLife.53968.
  PUBMED    32412410
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-05-15"; volume: "9. pii: 53968. doi"; pages: " 
            10.7554/eLife.53968"
COMMENT     Sequence Label: pPRISM-Cre-cmlc2-TagBFP
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggtctggaca cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa
       61 taccggagat catgcaagct ggtggctgga ccaatgtaaa tattgtcatg aactatatcc
      121 gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc gattagaact
      181 agtaattaag tctcagccac cgttaactga acatgtcaaa acctgtggag actgttgaga
      241 tttgatgttc tgaaaagata aagcctataa ataaaatgtt gcccaaattt cctgcctgat
      301 gtttttcttt gtctttgcta catggctttg ctgctcggat cggctcactc tgtgtatgcc
      361 acgttcactt tgtactctcc ttctcacggt aggtttatta tttttagatg tgcagttagt
      421 ttctgtgaaa taacacacca cacactgata ttgtctgtgc attgacttgg tgagtgcaca
      481 ttgtttttga tcttgacata tttatatttg attgatcagg tgaactgtgt gaatctaaag
      541 tgctccatac agatgttctg cattgaaaat attctcattt tattagtgga agtgagtgta
      601 tgccacatcc aatcaatttc agcaaacacc ccagtatgat ttaatgcaaa aaaatgaagg
      661 tatcaaacac gcattactac tttgcagtta aatatttaac atttattcca acacgaaaaa
      721 aagcagtaaa taacactttg acaaacacgt caggacatct tatttttgtc accctcacag
      781 gcaatttagt ataatatatt atatatatat atatatcata taataatatt cagtataata
      841 tatatatata tatcatatta taatattcag tataatataa aacacaaaca catatatgta
      901 taatataata taacattttt atttattgag atgcctctat ggaccgtgtt ataagaagta
      961 aagatcagga gaagtaaaca tgaagtgtaa ttatgaatac tgatgttaaa ttaagctatg
     1021 atgagttt
//
