LOCUS       sequence-23016-e         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B
  TITLE     PUMA induces the rapid apoptosis of colorectal cancer cells.
  JOURNAL   Mol Cell. 2001 Mar . 7(3):673-82.
  PUBMED    11463391
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2001 Mar . 7(3):673-82."
COMMENT     Sequence Label: 4XBS2WT-Luc
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    293..384
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(325..344)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    398..446
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 tcgcgganga tggcgccggg cctttcttta tgtttttggc gtcttccatg gtggctttac
       61 caacagtacc ggaatgccaa gcttacttag atcgcagatc tcgataccgg atccattata
      121 tacccgaatt cgatatcgac gtccagctgt cgagcccggg ctagggacaa gtcaggactt
      181 gcagagtgtg gacaagtcag gacttgcagc ctagggacaa gtcaggactt gcagagtgtg
      241 gacaagtcag gacttgcagc ctagcacgcg taagagctcg gtacctatcg atagagaaat
      301 gttctggcac ctgcacttgc actggggaca gcctattttg ctagtttgtt ttgtttcgtt
      361 ttgttttgat ggagagcgta tgttagtact atcgattcac acaaaaaacc aacacacaga
      421 tgtaatgaaa ataaagatat tttattgcgg ccgctccaag tacctcccgt accttaatat
      481 tacttactta tcatggtagc ttgggctggc gtaatagcga agaggcccgc accgatcgcc
      541 cttcccaaca gttgcgcagc ctgaatggcg aatggcaaat tgtaagcgtt aatattttgt
      601 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
      661 gcaaaatccc ttataaatca aaagaataga ccgagatngg gttgagtgtt
//
