LOCUS       sequence-230160-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor for precise CRISPR directed genomic integration using the 
            GeneWeld method.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Wierson WA, Welker JM, Almeida MP, Mann CM, Webster DA, Torrie ME, 
            Weiss TJ, Kambakam S, Vollbrecht MK, Lan M, McKeighan KC, Levey J, 
            Ming Z, Wehmeier A, Mikelson CS, Haltom JA, Kwan KM, Chien CB, 
            Balciunas D, Ekker SC, Clark KJ, Webber BR, Moriarity BS, Solin SL, 
            Carlson DF, Dobbs DL, McGrail M, Essner J
  TITLE     Efficient targeted integration directed by short homology in 
            zebrafish and mammalian cells.
  JOURNAL   Elife. 2020 May 15;9. pii: 53968. doi: 10.7554/eLife.53968.
  PUBMED    32412410
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-05-15"; volume: "9. pii: 53968. doi"; pages: " 
            10.7554/eLife.53968"
COMMENT     Sequence Label: pGTag-NLS-TagRFP-B-actin
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..24
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5..23
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 catgtaatac gactcactat aggggatatc gcggccgaga tcccatcgct agcgggaggc
       61 gttcgggcca cagcggagaa gagcatattc aatgtcgtgc ctacaacgag taacgtaacg
      121 ttacagtcat taccacctga catagccagt acagttatga taacatacat atatatccgg
      181 atagctcttc acttccttgc tagacaacgc catggtaatt tatttagcag tagatagcta
      241 tattgtgtga aacgcaaggt tatatggaca agaatttata tcagtcatgg tggtttgact
      301 tttaaactgg cagttccttc ctgttaaagc ctttatgtag ctcaacttaa gatggaaagt
      361 taaggccgtt tgcagttagc taccttcata tgagggaaca cttggtgtat tacttgaagt
      421 ttgtttcacc attaagcagt taaataaatg aatcatgttt cgaatttatg cttttaaaca
      481 ttaagtttcc tccaatgaaa tggtttgata acctcaaatt agcttgaaga aaaaaaaaaa
      541 aaaaaaggtc aaacttcgat aaattcaaat tagtcaaaaa cctggtgtga aagtgctgaa
      601 atgtaattaa catggtgcaa cttcacaaaa gccattttta ttgaagccac tcagagccaa
      661 gacaggaaat gagacagggg taggatgtct tacatgtgca cttttattgg aattgaatta
      721 gtcagtgtac aggttggccc caccaaatgt ctggcaccgc cacgttccac acagctttta
      781 caatatgccc agggggaatg gaaaccttta catcttgaat ggtaaagctt cgtttttttg
      841 gaacaaagaa aaaaaaataa acccaagatt ttaaggctga aaagtacaaa aacccagaca
      901 ttgttttgtt acataattta catacaatac tacaatgtta agtatgctcc caattactgg
      961 gcagccttca tagaggcaaa taagtttcgg aacaatgcat ttaacaaaca tttggaatga
     1021 ctaaagaaaa c
//
