LOCUS       sequence-230162-       10779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses recombinant MVA pathway in Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10779)
  AUTHORS   Jervis AJ, Carbonell P, Vinaixa M, Dunstan MS, Hollywood KA, 
            Robinson CJ, Rattray NJW, Yan C, Swainston N, Currin A, Sung R, 
            Toogood HS, Taylor S, Faulon JL, Breitling R, Takano E, Scrutton NS
  TITLE     Machine learning of designed translational control allows predictive
            pathway optimisation in Escherichia coli.
  JOURNAL   ACS Synth Biol. 2018 Dec 18. doi: 10.1021/acssynbio.8b00398.
  PUBMED    30563328
REFERENCE   2  (bases 1 to 10779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2018 Dec 18. doi: 10.1021/acssynbio.8b00398."
COMMENT     Sequence Label: pMVA2
FEATURES             Location/Qualifiers
     source          1..10779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..84
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             85..1167
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(104..123)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1399..1404,1405..1421,1422..1428)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1436..1452
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(5094..5099,5100..5116,5117..5123)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    5131..5147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      8708..8755
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      8789..8860
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8876..8903
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(8931..8948)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9065..9610)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(9145..9164)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(9724..9818)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(9849..10643)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9960..9979)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10570..10589
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gacgtcgaca ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa
       61 gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat
      121 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
      181 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
      241 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
      301 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
      361 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
      421 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
      481 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
      541 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
      601 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
      661 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
      721 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
      781 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
      841 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga
      901 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat
      961 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     1021 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
     1081 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1141 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agcgcgaatt
     1201 gatctggttt gacagcttat catcgactgc acggtgcacc aatgcttctg gcgtcaggca
     1261 gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct
     1321 caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa
     1381 atattctgaa atgagctgtt gacaattaat catccggctc gtataatgtg tggaattgtg
     1441 agcggataac aatttcagaa ttcaaaagat cttttaagaa ggagatatac atatgaaaac
     1501 cgtggtgatt attgatgcac tgcgtacccc gattggtaaa tacaaaggta gcctgagcca
     1561 ggttagcgca gttgatctgg gcacccatgt taccacccag ctgctgaaac gtcatagcac
     1621 cattagcgaa gaaattgatc aggtgatttt tggcaatgtt ctgcaggcag gtaatggtca
     1681 gaatccggca cgtcagattg caattaatag cggtctgagc catgaaattc cggcaatgac
     1741 cgttaatgaa gtttgtggta gcggtatgaa agcagttatt ctggcaaaac agctgatcca
     1801 gctgggcgaa gccgaagttc tgattgccgg tggtattgaa aatatgagcc aggcaccgaa
     1861 actgcagcgt ttcaattatg aaaccgaaag ctatgatgca ccgtttagca gcatgatgta
     1921 tgatggtctg accgatgcat ttagcggtca ggcaatgggt ctgacagcag aaaatgttgc
     1981 agaaaaatat catgtgaccc gtgaagaaca ggatcagttt agcgttcata gccagctgaa
     2041 agcagcacag gcacaggccg aaggtatttt cgcagatgaa attgcaccgc tggaagttag
     2101 cggcaccctg gttgaaaaag atgaaggtat tcgtccgaat agcagcgttg aaaaactggg
     2161 tacactgaaa acggtgttta aagaagatgg caccgttacc gcaggcaatg caagtaccat
     2221 taatgatggt gcaagcgcac tgattattgc cagccaagaa tatgccgaag cacatggtct
     2281 gccgtatctg gcaattattc gtgatagcgt tgaagttggt attgatccgg catatatggg
     2341 tattagcccg attaaagcaa ttcagaaact gctggcacgt aatcagctga ccaccgaaga
     2401 aatcgacctg tacgaaatta atgaagcatt tgccgcaacc agcattgttg ttcagcgtga
     2461 actggcactg ccggaagaaa aagttaacat ttatggcggt ggcatcagcc tgggtcatgc
     2521 aattggtgca accggtgcac gtctgctgac cagcctgagc tatcagctga atcagaaaga
     2581 gaaaaaatac ggcgttgcaa gcctgtgtat tggtggtggc ctgggtctgg caatgctgct
     2641 ggaacgccct caacagaaaa aaaacagccg tttttatcag atgagtccgg aagaacgtct
     2701 ggccagcctg ctgaatgaag gtcagattag cgcagatacc aaaaaagaat ttgaaaacac
     2761 cgcactgagc agccagattg ccaaccacat gattgaaaat cagatcagcg aaaccgaagt
     2821 gccgatgggt gttggtctgc atctgaccgt ggatgaaacg gattatctgg ttccgatggc
     2881 aaccgaagaa ccgagcgtta ttgcagccct gagcaatggt gcaaaaattg cacagggctt
     2941 taaaaccgtg aatcagcagc gtctgatgcg tggtcagatt gttttttatg atgttgccga
     3001 tgcagaaagc ctgattgatg aactgcaggt tcgtgaaaca gaaattttcc agcaggcaga
     3061 actgagttat ccgagcattg ttaaacgcgg tggtggtctg cgtgatctgc agtatcgtgc
     3121 atttgatgaa agttttgtta gcgtggattt tctggtggat gttaaagacg caatgggtgc
     3181 caatattgtt aatgcaatgc tggaaggtgt tgccgaactg tttcgtgaat ggtttgcaga
     3241 acaaaaaatc ctgtttagca tcctgagtaa ctatgccacc gaaagcgttg ttaccatgaa
     3301 aacagcaatt ccggttagcc gtctgagcaa aggtagtaat ggtcgtgaaa ttgccgaaaa
     3361 aattgttctg gcaagccgtt atgccagcct ggatccgtat cgtgccgtta cccataataa
     3421 aggtattatg aatggcattg aagcagttgt gctggccacc ggtaatgata cccgtgcagt
     3481 tagcgcaagc tgtcatgcat ttgcagttaa agaaggtcgt tatcagggtc tgaccagctg
     3541 gaccctggat ggtgagcagc tgattggtga aattagcgtt ccgctggcac tggcaaccgt
     3601 tggtggtgcc accaaagttc tgccgaaaag ccaggcagca gccgatctgc tggcagttac
     3661 cgatgcaaaa gaactgagcc gtgttgttgc agcagttggt ctggcacaga atctggcagc
     3721 actgcgtgca ctggttagcg aaggcattca gaaaggtcac atggcactgc aggcacgttc
     3781 actggccatg accgtgggtg cgaccggtaa agaagttgaa gccgttgcac agcaactgaa
     3841 acgccagaaa acaatgaatc aggatcgtgc cctggcaatt ctgaatgatc tgcgtaaaca
     3901 gtaatgatta gcgacaaaat aaataacgag taaaaaatga ccattggcat cgacaaaatc
     3961 agcttttttg ttccgcctta ctatatcgac atgaccgcac tggccgaagc acgtaatgtt
     4021 gatccgggta aatttcatat tggtattggt caggatcaga tggccgttaa tccgattagc
     4081 caggatattg ttacctttgc agcaaatgca gcagaagcaa ttctgaccaa agaagataaa
     4141 gaagccatcg atatggttat tgttggcacc gaaagcagca ttgatgaaag caaagcagcc
     4201 gcagttgttc tgcatcgtct gatgggtatt cagccgtttg cacgtagctt tgaaattaaa
     4261 gaaggttgtt acggcgcaac cgcaggtctg cagctggcaa aaaatcatgt tgcactgcat
     4321 ccggataaaa aagttctggt tgttgcagca gatatcgcca aatatggtct gaatagcggt
     4381 ggtgaaccga cccagggtgc cggtgcagtt gcaatgctgg ttgcaagcga accgcgtatt
     4441 ctggcactga aagaggataa tgttatgctg acgcaggata tctatgattt ttggcgtccg
     4501 accggtcatc cgtatccgat ggttgatggt ccgctgagca atgaaaccta tattcagagc
     4561 tttgcacagg tgtgggatga acataaaaaa cgtaccggtc tggatttcgc agattatgat
     4621 gcactggcct ttcatattcc gtataccaaa atgggtaaaa aagcactgct ggcgaaaatt
     4681 agcgatcaga ccgaagccga acaagaacgt atcctggcac gttatgaaga aagcattatc
     4741 tatagccgtc gtgtgggtaa tctgtatacc ggtagcctgt atctgggtct gattagcctg
     4801 ctggaaaatg caaccaccct gaccgctggt aatcagattg gtctgtttag ctatggtagc
     4861 ggtgccgttg cagaattctt taccggtgaa ctggttgcag gttatcagaa tcatctgcag
     4921 aaagaaaccc atctggccct gctggataat cgtaccgaac tgagcattgc agaatatgaa
     4981 gcaatgtttg cagaaaccct ggataccgat attgatcaga ccctggaaga cgaattaaaa
     5041 tatagcatta gcgccattaa taacaccgtg cgtagctatc gtaattaaag ctgttgacaa
     5101 ttaatcatcc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cagacaggct
     5161 cccatttaac ataaggattt ttctacgatg accaaaaaag ttggtgttgg tcaggcacat
     5221 agcaaaatta tcctgattgg tgaacatgcc gtggtttatg gttatccggc aattagcctg
     5281 ccgctgctgg aagttgaagt tacctgtaaa gttgttagcg cagaaagccc gtggcgtctg
     5341 tatgaagagg ataccctgag catggcagtt tatgcaagcc tggaatatct ggatatcacc
     5401 gaagcatgtg ttcgttgtga aattgatagc gcaattccgg aaaaacgtgg tatgggtagc
     5461 agcgcagcaa ttagcattgc agcaattcgt gcagtgttcg attattatca ggccgatctg
     5521 ccgcatgatg ttctggaaat tctggttaat cgtgcagaaa tgattgccca catgaatccg
     5581 agcggtctgg atgcaaaaac ctgtctgagc gatcagccga ttcgttttat caaaaatgtg
     5641 ggttttaccg aactggaaat ggatctgagc gcatatctgg ttattgcaga taccggtgtt
     5701 tatggtcata cccgtgaagc aattcaggtt gttcagaata aaggtaaaga tgcactgccg
     5761 tttctgcatg cactgggtga actgacccag caggcagaag ttgccattag ccagaaatat
     5821 gcagaaggtc tgggtctgat ttttagccag gcacatctgc atctgaaaga aattggtgtt
     5881 agcagtccgg aagcagattt tctggttgaa accgcactga gctatggtgc cctgggtgca
     5941 aaaatgagcg gtggtggtct gggtggttgt attattgcac tggttaccaa tctgacccat
     6001 gcacaagaac tggcagaacg tctggaagaa aaaggtgccg ttcagacctg gattgaaagc
     6061 ctgtaaacaa actcaaagga aacatagaag gggcaaagga tgattgccgt taaaacctgc
     6121 ggtaaactgt attgggcagg cgaatacgca attctggaac cgggtcagct ggcactgatt
     6181 aaagatattc cgatttatat gcgtgccgaa atcgcattta gcgatagcta ccgtatttat
     6241 agcgatatgt ttgattttgc cgttgatctg cgtccgaacc cggattatag cctgattcaa
     6301 gaaaccattg cactgatggg tgattttctg gcagttcgtg gtcagaacct gcgtccgttt
     6361 agcctgaaaa tttgtggtaa aatggaacgc gagggcaaaa aattcggtct gggtagcagc
     6421 ggtagcgttg ttgttctggt tgttaaagca ctgctggccc tgtataatct gagcgttgat
     6481 cagaatctgc tgtttaaact gaccagcgca gttctgctga aacgtggtga taatggtagc
     6541 atgggtgatc tggcatgtat tgttgcagaa gatctggttc tgtatcagag ctttgatcgt
     6601 cagaaagcag cagcatggct ggaagaagaa aatctggcaa ccgttctgga acgtgattgg
     6661 ggctttttta tcagccaggt taaaccgacc ctggaatgtg atttcctggt tggttggacc
     6721 aaagaagttg cagttagcag ccacatggtt cagcagatta aacagaacat taaccagaac
     6781 tttctgtcca gcagcaaaga aaccgttgtt agcctggttg aagcactgga acagggtaaa
     6841 gcagaaaaag ttattgaaca ggttgaagtg gcaagcaaac tgctggaagg tctgagcacc
     6901 gatatctata caccgctgct gcgtcagctg aaagaagcaa gccaggatct gcaggcagtt
     6961 gcaaaaagca gtggtgccgg tggtggtgat tgtggtattg cactgtcatt tgatgcacag
     7021 agcagccgta ataccctgaa aaatcgttgg gcagatctgg gtattgaact gctgtatcaa
     7081 gaacgtattg gccacgacga taaaagctaa aaaacactag tccacaatta cttaaggagg
     7141 gtcctatgga tcgcgaaccg gttaccgttc gtagctatgc aaatattgcc atcatcaaat
     7201 attggggcaa aaaaaaagaa aaagagatgg ttccggcaac cagcagcatt agcctgaccc
     7261 tggaaaatat gtataccgaa accaccctga gtccgctgcc tgcaaatgtt accgcagatg
     7321 aattttacat taatggccag ctgcagaacg aagttgaaca tgcaaaaatg agcaaaatca
     7381 tcgatcgtta tcgtccggca ggcgaaggtt ttgttcgtat tgatacccag aataatatgc
     7441 cgaccgcagc aggtctgagc agcagcagta gcggtctgag cgcactggtt aaagcatgta
     7501 atgcatattt caaactgggt ctggatcgta gtcagctggc acaagaagca aaatttgcaa
     7561 gcggtagcag cagccgttca ttttatggtc cgctgggtgc atgggataaa gatagcggtg
     7621 aaatttatcc ggttgaaacc gatctgaaac tggcaatgat tatgctggtt ctggaagata
     7681 aaaaaaaacc gattagcagc cgtgatggta tgaaactgtg tgttgaaacc agcaccacct
     7741 ttgatgattg ggttcgtcag agcgaaaaag attatcagga tatgctgatc tatctgaaag
     7801 agaacgattt cgccaaaatt ggtgaactga ccgaaaaaaa tgccctggca atgcatgcaa
     7861 ccaccaaaac cgcaagtccg gcatttagct atctgaccga tgcaagctat gaagcaatgg
     7921 catttgttcg tcagctgcgt gaaaaaggtg aagcatgtta ttttacgatg gatgcaggtc
     7981 cgaacgtgaa agttttttgc caagagaaag atctggaaca cctgagcgaa atttttggtc
     8041 agcgttatcg cctgattgtg agcaaaacca aagatctgag ccaggatgat tgttgctaag
     8101 gagtcgcacg agacgccatt taaggagcca ggcgatgcag accgaacatg ttattctgct
     8161 gaatgcacag ggtgttccga ccggcaccct ggaaaaatat gcagcacata ccgcagatac
     8221 ccgtctgcat ctggcattta gcagctggct gtttaatgca aaaggtcagc tgctggttac
     8281 ccgtcgtgca ctgagcaaaa aagcatggcc tggtgtttgg accaatagcg tttgtggtca
     8341 tccgcagctg ggtgaaagca atgaagatgc agttattcgt cgttgtcgtt atgaactggg
     8401 tgttgaaatt acccctccgg aaagcattta tccggatttt cgttatcgtg caaccgatcc
     8461 gagcggtatt gttgaaaatg aagtttgtcc ggtttttgca gcacgtacca cctcagcact
     8521 gcagattaat gatgatgaag tgatggatta tcagtggtgt gatctggcag atgttctgca
     8581 tggtattgat gcaaccccgt gggcctttag cccgtggatg gttatgcagg caaccaatcg
     8641 tgaagcacgt aaacgtctga gcgcatttac ccagctgaaa taagagatcc ggctgtggct
     8701 gctgccacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgttctg
     8761 tcgacctcga gtaaggatct ccaggcatca aataaaacga aaggctcagt cgaaagactg
     8821 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     8881 accttcgggt gggcctttct gcgtttatac ctagggatat attccgcttc ctcgctcact
     8941 gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga
     9001 gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc
     9061 cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt
     9121 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg
     9181 tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt
     9241 tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt
     9301 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9361 tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga
     9421 ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga
     9481 ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga
     9541 aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa
     9601 acgatctcaa gaagatcatc ttattaatca gataaaatat ttctagattt cagtgcaatt
     9661 tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt tactagtgct
     9721 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     9781 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctct cgaaccccag
     9841 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     9901 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     9961 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag
    10021 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
    10081 tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg
    10141 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
    10201 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
    10261 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
    10321 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
    10381 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
    10441 gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca
    10501 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
    10561 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
    10621 tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatcat
    10681 gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc
    10741 ttcccaacct taccagaggg cgccccagct ggcaattcc
//
