LOCUS       sequence-230174-        7864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesion Kinase, Excitation: 400 / 504, 
            Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7864)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 7864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-FAK-5
FEATURES             Location/Qualifiers
     source          1..7864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             553..1260
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(698..716)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    4592..4713
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4629..4648)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4683..4702
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4720..5175)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4857..4878)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5069..5088
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5202..5306
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5308..5665
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5308..5499
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5331..5351)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5516..5651
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5700..6494
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5700..6494
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5754..5773)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6364..6383
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6682..6701)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6726..6773
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7102..7690
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7591..7610
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat taaggagttc
      601 atgcgcttca aggtgcacat ggaggggtcc gtgaacggcc acgtgttcga gatcgagggc
      661 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
      721 ggccccctgc ccttcacctg ggacatcctg agccctcagt tcatgtacgg ctccaatgcc
      781 tacgtgaagc accccgccga catccccgac tactttaagc tgtccttccc cgagggcttc
      841 aagtgggagc gcgtgatgaa attcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      901 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
      961 gacggccccg tgatgcagaa gaagaccatg ggctgggagg ccctctccga gcggatgtac
     1021 cccgaggacg gcgccctgaa gggcgaggtc aagcccagag tgaagctgaa ggacggcggc
     1081 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1141 gcctacaacg tcaaccgcaa gctggacatc accagccaca acgaggacta caccatcgtg
     1201 gagcagtacg agagagccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1261 tccggactca gatctatggc agcagcttac cttgatccaa acttgaatca tacaccaagt
     1321 tcaagtgcaa agacgcacct cggtactggg atggagcgtt ccccgggggc catggagcga
     1381 gtcctaaagg tttttcacta ctttgaaaac agcagcgagc caacgacgtg ggccagcatt
     1441 atccggcatg gagatgctac tgatgttcga ggcataatac agaagattgt ggactgtcac
     1501 aaagtgaaaa atgtggcctg ctatgggttg cgactcagtc atctgcagtc tgaggaggtt
     1561 cactggctgc acctggacat gggggtatcc aatgtgagag agaaatttga actagcacat
     1621 cctccagaag aatggaaata tgaactgaga attcggtacc tgcccaaagg atttctaaac
     1681 cagttcactg aggacaaacc aactttaaat tttttctatc agcaggtgaa aaatgactat
     1741 atgttagaaa tagcagatca agtggaccag gaaattgctt tgaaactagg ttgccttgaa
     1801 atcaggagat cctacggaga gatgagaggc aatgcattag agaagaaatc caactatgaa
     1861 gtgctagaaa aagatgtcgg tttaagacga ttttttccga agagtttgct agattcagtg
     1921 aaggccaaaa cactacgaaa attaatccaa cagacatttc gacaatttgc caacctcaac
     1981 agagaagaaa gtattttgaa attctttgag atcctctctc cagtgtacag atttgacaag
     2041 gaatgcttca agtgtgccct tggttcaagc tggattattt cagtggagct ggcaattggc
     2101 ccagaggaag gaatcagcta ccttacagac aagggtgcaa atccaactca cctggcagat
     2161 tttaatcaag tacaaactat tcagtattca aacagtgaag acaaggacag aaaagggatg
     2221 ttgcaactga agatagctgg tgcacctgag cctctgacag tgacagcacc atccttaacc
     2281 attgcagaga atatggctga cttgatagac ggatactgcc gactggtgaa tggagccacg
     2341 caatctttta ttatcaggcc acagaaagaa ggtgaaagag ctttaccatc aataccaaag
     2401 ctggccaaca atgagaagca aggagtaagg tcgcacacag tctctgtatc agaaacagat
     2461 gactatgcag agataataga tgaagaagat acttatacaa tgccatcaac cagagattat
     2521 gaaattcaaa gggagagaat tgaactgggg cgctgcattg gtgaaggaca gtttggagat
     2581 gtgcaccaag gaatttacat gagtccggaa aatccagcta tggctgtagc aatcaaaaca
     2641 tgtaaaaact gcacctcaga cagcgttaga gaaaagttcc tacaagaagc cttaacaatg
     2701 cgtcagtttg atcatcctca cattgtgaag ctcattggag ttattacaga aaacccagtg
     2761 tggataatca tggagctctg tacacttgga gagttgagat cgtttctgca agtaagaaaa
     2821 ttcagcttgg acctggcctc cctcatcctc tacgcttacc agcttagcac agcacttgct
     2881 tacctagaga gcaaaagatt tgtacataga gatattgctg ctaggaacgt gctggtatct
     2941 gccactgact gtgtgaaatt gggtgacttt ggcttatccc gatacatgga agacagtact
     3001 tactataaag cttccaaagg aaagttacct atcaaatgga tggctccaga gtcaatcaac
     3061 ttccgacggt ttacctcagc aagcgatgtg tggatgtttg gtgtgtgtat gtgggagatc
     3121 ctgatgcatg gggtaaagcc cttccaggga gtgaaaaata atgatgttat tggtcggatt
     3181 gagaacggtg agcggctccc catgcctccg aactgccctc ccaccctcta cagccttatg
     3241 accaagtgct gggcatacga ccctagtaga cgacccaggt ttactgaact taaagcacaa
     3301 ctcagtacaa tactggagga ggagaagctg cagcaagagg aacgaatgag aatggaatcc
     3361 aggcgacaag tcacagtatc ctgggactca ggaggatcag atgaagctcc tcccaagccc
     3421 agcaggcctg gttaccccag cccaaggtcc agtgaagggt tttatccgag tcctcagcat
     3481 atggtacagc caaatcacta ccaggtatct ggctactctg gttctcatgg gataccagcc
     3541 atggcaggca gcatttatcc tgggcaagct tctctcttgg atcaaacaga ttcctggaac
     3601 catcgacctc aggaagtatc agcatggcag ccaaacatgg aggattcggg cactttggat
     3661 gtacgaggaa tggggcaggt tctgcccaca catctcatgg aggagaggtt aataagacaa
     3721 cagcaagaga tggaagaaga tcaacgctgg cttgagaaag aggaacgatt cctggtaatg
     3781 aaacctgatg tgcggctctc cagaggcagc attgaacggg aggacggagg tctccagggc
     3841 ccagctggta accagcacat atatcagcct gtgggtaaac cagatcatgc cgctccacca
     3901 aagaagcccc ctcgccctgg agccccccac ttgggcagcc tcgcgagcct gaacagcccc
     3961 gtggacagct acaacgaagg cgtgaagatc aagccacagg aaatcagccc tcctcctacg
     4021 gccaacctgg accgctccaa tgacaaagtc tatgagaatg taaccgggct ggtgaaagct
     4081 gtcatagaga tgtccagtaa aatacagcca gctccgccag aggagtacgt gcccatggta
     4141 aaggaggttg gcttggcgct gagaaccttg ctagcaacag tggatgagtc gctgccagtg
     4201 cttcctgcaa gcacccacag agagattgag atggcccaga aactgctgaa ctctgacctg
     4261 gctgagctca ttaacaagat gaagctggcc cagcagtacg tcatgaccag cctgcagcag
     4321 gagtacaaga agcaaatgct gacggctgct cacgctctgg ctgtggatgc caagaacttg
     4381 ctggatgtca tcgatcaagc cagactgaaa atgatcagcc agtccaggcc cactaagctg
     4441 ccgtagccga attctgcaga tgggatccac cggatctaga taactgatca taatcagcca
     4501 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     4561 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     4621 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     4681 ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt
     4741 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     4801 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     4861 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     4921 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     4981 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     5041 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     5101 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     5161 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5221 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5281 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     5341 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5401 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5461 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     5521 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5581 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     5641 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca
     5701 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     5761 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     5821 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     5881 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     5941 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     6001 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     6061 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     6121 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     6181 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     6241 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     6301 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     6361 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     6421 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     6481 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     6541 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     6601 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     6661 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     6721 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     6781 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     6841 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     6901 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact
     6961 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7021 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7081 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7141 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7201 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7261 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7321 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7381 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7441 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7501 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7561 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7621 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7681 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7741 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat
     7801 gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     7861 tccg
//
