LOCUS       sequence-230175-       10567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in 
            pBbaA5k backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Shomar H, Gontier S, van den Broek NJF, Tejeda Mora H, Noga MJ, 
            Hagedoorn PL, Bokinsky G
  TITLE     Metabolic engineering of a carbapenem antibiotic synthesis pathway 
            in Escherichia coli.
  JOURNAL   Nat Chem Biol. 2018 Aug;14(8):794-800. doi: 
            10.1038/s41589-018-0084-6. Epub 2018 Jun 25.
  PUBMED    29942079
REFERENCE   2  (bases 1 to 10567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-018-0084-6"; journalName: "Nat Chem Biol"; date: 
            "2018-08"; volume: "14"; issue: "8"; pages: "794-800"
COMMENT     Sequence Label: pCarCBADE_ProB(I69E)A
FEATURES             Location/Qualifiers
     source          1..10567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      7045..7116
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7132..7159
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(7317..7905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7397..7416)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(7993..8087)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(8118..8912)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8229..8248)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8839..8858
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(9097..10179)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     10141..10160
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(10180..10257)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     10463..10482
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
       61 gaattcaaaa gatctaggag gaaaaaaatg agtgacagcc agacgctggt ggtaaaactc
      121 ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc gtgcccatat cgttgaactt
      181 gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga ttgttattgt gacgtcgggc
      241 gcgatcgccg ccggacgtga gcacctgggt tacccggaac tgccagcgac tgaagcctcg
      301 aaacaactgc tggcggcggt agggcagagt cgactgattc aactgtggga acagctgttt
      361 tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc gtgctgatat ggaagaccga
      421 gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc tcgataacaa tatcgttccg
      481 gtaatcaatg agaacgatgc tgtcgctacg gcagagatta aggtcggcga taacgataac
      541 ctttctgcgc tggcggcgat tctggcgggt gccgataaac tgttgctgct gaccgatcaa
      601 aaaggtttgt ataccgctga cccgcgcagc aatccgcagg cagaactgat taaagatgtt
      661 tacggcattg atgacgcact gcgcgcgatt gccggtgaca gcgtttcagg cctcggaact
      721 ggcggcatga gtaccaaatt gcaggccgct gacgtggctt gccgtgcggg tatcgacacc
      781 attattgccg cgggcagcaa gccgggcgtt attggtgatg tgatggaagg catttccgtc
      841 ggtacgctgt tccatgccca ggcgactccg cttgaaaacc gtaaacgctg gattttcggt
      901 gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa ctgccgccat tctggaacgc
      961 ggcagctccc tgttgccgaa aggcattaaa agcgtgactg gcaatttctc gcgtggtgaa
     1021 gtcatccgca tttgcaacct cgaaggccgc gatatcgccc acggcgtcag tcgttacaac
     1081 agcgatgcat tacgccgtat tgccggacac cactcgcaag aaattgatgc aatactggga
     1141 tatgaatacg gcccggttgc cgttcaccgt gatgacatga ttacccgtta aggagcaggc
     1201 tgatgctgga acaaatgggc attgccgcga agcaagcctc gtataaatta gcgcaactct
     1261 ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga tgaactggaa gcacaaagcg
     1321 aaatcatcct caacgctaac gcccaggatg ttgctgacgc gcgagccaat ggccttagcg
     1381 aagcgatgct tgaccgtctg gcactgacgc ccgcacggct gaaaggcatt gccgacgatg
     1441 tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt aatcgatggc ggcgtactgg
     1501 acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg ggttattggc gtgatttatg
     1561 aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg cctgaaaacc ggtaatgcgg
     1621 tgatcctgcg cggtggcaaa gaaacgtgtc gcactaacgc tgcaacggtg gcggtgattc
     1681 aggacgccct gaaatcctgc ggcttaccgg cgggtgccgt gcaggcgatt gataatcctg
     1741 accgtgcgct ggtcagtgaa atgctgcgta tggataaata catcgacatg ctgatcccgc
     1801 gtggtggcgc tggtttgcat aaactgtgcc gcgaacagtc gacgatcccg gtgatcacag
     1861 gtggtatagg cgtatgccat atttacgttg atgaaagtgc agagatcgct gaagcattaa
     1921 aagtgatcgt caacgccaaa actcagcgtc cgagcacatg taatacggta gaaacgttgc
     1981 tggtgaataa aaacatcgcc gatagcttcc tgcccgtatt aagcaaacaa atggcggaaa
     2041 gcggcgtgac attacacgca gatgcagctg cgctgacaca gttgcaggca ggccccgcga
     2101 aggtggtggc ggttaaagcc gaagagtatg acgatgagtt tctgtcatta gatttgaacg
     2161 tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat tcgtgaacac ggcacgcaac
     2221 actccgatgc gatcctgacc cgcgatatgc gcaacgccca gcgttttgtt aacgaagtgg
     2281 attcgtccgc tgtttacgtt aacgcctcta cgcgttttac cgacggcggc cagtttggac
     2341 tgggtgcgga agtggcggta agcacacaaa aactgcacgc acgtggccca atggggctgg
     2401 aagcactgac cacttacaag tggatcggca ttggtgatta caccattcgt gcgtaatagg
     2461 aggaaaaaaa tgtccgagat cgtgaaattt aaccctgtga tggcttctgg cttcggcgca
     2521 tacattgatc accgtgactt cctggaagct aaaacggaaa cgatcaaaaa cctgctgatg
     2581 cgccaaggtt tcgtggttgt caaaaacctg gatattgact ccgatacttt ccgtgatatc
     2641 tactctgcat acggtaccat cgtcgaatac gcggatgaaa aaattggtgt tggttttggt
     2701 tatcgcgata ccctgaaact ggaaggcgaa aaaggtaaaa tcgttaccgg tcgtggtcaa
     2761 ctgcctttcc acgctgacgg tggtctgctg ctgtctcagg tcgatcaggt tttcctgtac
     2821 gcggcggaga ttaaaaacgt caaattccgc ggtgcaacta ccgtttgcga tcacgctctg
     2881 gcgtgccagg aaatgccagc acacctgctg cgcgttctgg aagaggagac gttcgaagtt
     2941 cgtgtgctgg aacgtggtta ctacgtggat gtgtcccctg atggttggtt caaagtgccg
     3001 gtgttcactg atctgggttg ggttcgtaaa atgctgatct atttcccatt cgatgaaggc
     3061 cagccggcaa gctgggaacc gcgtattgtt ggttttaccg accacgaaac ccaggcattc
     3121 tttcaagaac tgggtgcgtt cctgaaacag ccgcgttatt actacaaaca tttctgggaa
     3181 gacggtgacc tgctgattat ggataaccgt cgtgttatcc acgagcgtga agaatttaac
     3241 gatgatgaca tcgttcgtcg tctgtaccgc ggccaaactg cagatatcta aaggaggaaa
     3301 aaatggtatt cgaagaaaac tccgatgaag tacgcgtgat taccctggat cacccgaaca
     3361 aacataaccc attttcccgt accctggaaa ctagcgtgaa ggacgctctg gctcgtgcaa
     3421 acgcggatga tagcgtgcgt gcggtcgttg tgtatggcgg cgctgaacgt agcttcagcg
     3481 caggcggtga tttcaacgaa gtcaagcagc tgtctcgctc cgaagatatt gaagaatgga
     3541 tcgatcgtgt gatcgacctg tatcaggcag tcctgaacgt aaacaaaccg acgatcgcgg
     3601 ccgttgatgg ctacgcgatc ggtatgggct tccaattcgc tctgatgttt gaccagcgcc
     3661 tgatggccag caccgcgaac ttcgtgatgc cggaactgaa acacggtatc ggttgctctg
     3721 taggtgcagc aatcctgggt ttcacccatg gtttctccac catgcaagaa atcatctacc
     3781 agtgccagtc tctggatgca ccgcgctgcg tcgactaccg cctggtgaat caggtcgtag
     3841 agtcttccgc gctgctggac gcggcgatca ctcaggcgca cgtgatggcg tcctacccgg
     3901 cttccgcatt cattaacacg aagcgtgcgg taaacaagcc gttcattcac ctgctggaac
     3961 agacccgtga tgcttccaaa gcagtgcaca aagccgcatt ccaggcgcgt gatgcccagg
     4021 gtcacttcaa aaacgttctg ggtaagaaat actgaggatc taggaggaaa aaaatgagca
     4081 atagcttctg cgtcgtttac aaaggttccg atactgacat taacaacatt cagcgtgatt
     4141 tcgacggtaa aggcgaagcc ctgtctaacg gctatctgtt catcgaacag aacggccact
     4201 accagaaatg cgaaatggag cgtggcacgg cgtacctgat tggttctctg tataaccgta
     4261 ctttcctgat tggcctggcg ggcgtttggg aaggtgaagc atacctggct aacgatgctg
     4321 aactgctggc cctgctgttt acgcgcctgg gtgccaacgc actggctctg gcggaaggcg
     4381 atttctgctt tttcattgat gaaccgaatg gtgagctgac cgttatcacg gaatctcgcg
     4441 gcttctcccc ggtccatgtt gttcaaggta agaaggcatg gatgacgaac agcctgaaac
     4501 tggtaactgc tgccgaaggc gagggcgcac tgtggtttga agaagaagct ctggtgtgcc
     4561 agtctctgat gcgtgccgat acttatacgc cggttaagaa cgcgcagcgt ctgaaaccgg
     4621 gcgcagtgca cgttctgacc cacgatagcg aaggctacag ctttgtagag tctcgtaccc
     4681 tgaccactcc ggcatccaac caactgctgg ctctgccgcg tgaaccgctg ctggcactga
     4741 tcgatcgcta cctgaacgct ccgctggagg acctggcacc acgcttcgac acggttggta
     4801 tcccgctgag cggtggtctg gactcttccc tggtgactgc cctggctagc cgccatttca
     4861 agaaactgaa cacgtattcc atcggtactg agctgtccaa cgagtttgag ttcagccagc
     4921 aggttgcaga tgctctgggt acgcaccacc agatgaaaat cctgtccgaa acggaagtta
     4981 ttaacggtat catcgagtcc atttactaca acgaaatctt cgacggtctg agcgcggaaa
     5041 tccagagcgg tctgttcaac gtgtaccgtc aggctcaggg ccaagtctcc tgcatgctga
     5101 ctggctatgg ttctgacctg ctgttcggcg gcatcctgaa acctggcgct cagtatgata
     5161 atccgaacca gctgctggcg gaacaagtgt accgcacccg ttggaccggc gaatttgcga
     5221 cccacggtgc gtcttgctac ggcatcgata tccgccaccc gttttggtcc cacagcctga
     5281 ttagcctgtg ccacgccctg caccctgatt acaagatttt cgataatgaa gttaaaaaca
     5341 tcctgcgtga atacgctgat agcctgcagc tgctgccaaa ggacatcgtt tggcgtaaaa
     5401 agatcggtat tcacgaaggt tcttctgtta atcaggcgtt cgcaaacgta ctgggttcta
     5461 cggttgacaa ttaccagacc aaatctcgtt tcacttatcg tgtttaccag gcgttcctgc
     5521 gcggtcgcct gtccatcact gacgttaccc cgtcccagct gaaggatctg atcaaaaagg
     5581 actgaggatc taggaggaaa aaaatgccta acgatcttta cgctatctac aatcgttaca
     5641 cgtcaaggac cctgtttttt aaatactgtg cgacagcgac tctaacgcac agactgacac
     5701 gtcggctgtc gctttttacg ttgaaaaagt gccttgcccg tccacaggga cgccttttct
     5761 ctctggttaa tagcatttat tttggcggag aaacgctgga agaggttcag agtacagcaa
     5821 cttttctggc acgctctggc attgcttgcg tactggacta cgccgtggaa ggggaaaatg
     5881 acgagacgca gttcgataag gcaatggaaa atacgctgcg ccttatcgag atgtcgcagc
     5941 aaacggatag cctgcctttt gtcgtgatta aaccgtcgtc tctgggtagt gttgctgtgt
     6001 atgctcggca aagtgaaagg ctcgcgctgg atgaagcatc tgcaagtgca tggtcacgca
     6061 tcgttacgcg tttctcacgt ctgttcgatt atgcccgctc tcatggcgtg catgtgatgg
     6121 ttgatgcaga gcagaccgcg attcagcccg cagtggatcg tttggttcta gacatgatgc
     6181 gtgaatttaa ccgcgatagc gcggtgatca cgctgacgct gcaattttat ctaaaggatc
     6241 aattgcgttt tctcgacgaa tgctatcagc gagcctgtca ggataatttc ctgttcggcg
     6301 tgaaagtagt gcgcggtgct tatctggaag aagagaaacg agtgaacggt ggcgtacgct
     6361 gttttgcaac caaacaggaa accgatcgta gttataacgc ggcggtggat tacattgcac
     6421 tgcgtctgga tcgaatcgca ccgtttttcg ccacgcacaa cgaggaaagc ctcgctttga
     6481 ttatgtcgag tgagtcgcta cgcgcggggc gcacctgggt aggccagctt tacgggttgg
     6541 gcgatcatat tacttattcg ctgctgcaaa ctggtttccg cgtgtgcaaa tacctgcctt
     6601 acggtccgct cgataagtcg ttgccctatt tactccgcag gattgaagag aacgcggttg
     6661 cctcggcaac cttcaaaaag gaaaataaac tcttgcagaa ggagttgctg cgccgtctcg
     6721 taggagggat gtaatgtcaa aatttcattg tcatattcgc gatacggcta tcggtttccc
     6781 cgtgtcggag gatgaaagcg tactcagttc agcctatgaa gctggtgtcg aactgcctta
     6841 tcgctgtgct tctggttact gtggtgtatg caaggtgcgc ctgacgtccg gcaacgtcaa
     6901 catggatcat tctggtggga tctcacgtaa agatatcgct gatggttaca ttctaccttg
     6961 ctgttctgtg ccgctctcta acctcgaaat tgaacctgta tcgtcatgct gaggatccaa
     7021 actcgagtaa ggatctccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     7081 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     7141 tcgggtgggc ctttctgcgt ttatacctag ggcgttcggc tgcggcgagc ggtatcagct
     7201 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7261 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7321 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7381 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7441 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7501 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7561 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7621 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7681 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7741 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7801 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7861 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7921 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7981 actagtgctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     8041 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctctc
     8101 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     8161 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     8221 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
     8281 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     8341 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg
     8401 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     8461 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     8521 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     8581 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     8641 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     8701 gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg
     8761 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     8821 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     8881 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
     8941 tcagatcatg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact
     9001 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccga cgtcggtgcc
     9061 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     9121 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     9181 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
     9241 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
     9301 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc
     9361 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
     9421 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
     9481 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
     9541 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
     9601 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
     9661 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
     9721 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
     9781 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
     9841 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
     9901 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
     9961 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
    10021 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
    10081 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
    10141 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact
    10201 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg
    10261 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
    10321 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
    10381 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
    10441 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
    10501 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gtttaggcac
    10561 cccaggc
//
