LOCUS       sequence-230198-        2381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the SthK channel.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Bernal Sierra YA, Rost BR, Pofahl M, Fernandes AM, Kopton RA, Moser 
            S, Holtkamp D, Masala N, Beed P, Tukker JJ, Oldani S, Bonigk W, Kohl
            P, Baier H, Schneider-Warme F, Hegemann P, Beck H, Seifert R, 
            Schmitz D
  TITLE     Potassium channel-based optogenetic silencing.
  JOURNAL   Nat Commun. 2018 Nov 5;9(1):4611. doi: 10.1038/s41467-018-07038-8.
  PUBMED    30397200
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-11-5"; volume: "9(1):4611. doi"; pages: " 
            10.1038/s41467-018-07038-8"
COMMENT     Sequence Label: pAAV-CamkII-Sthk-p2a-tpPAC minWPRE
FEATURES             Location/Qualifiers
     source          1..2381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     139..156
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    273..294
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(309..314,315..332,333..339)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    347..363
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     352..374
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     371..387
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     371..387
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     406..426
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        408..426
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     463..479
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     463..479
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(556..574)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        642..746
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(747..815,816..1607)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(965..984)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1778..2366
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2267..2286
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
       61 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
      121 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
      181 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
      241 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
      301 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      361 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
      421 taaagggaac aaaagctgga gctccaccgc ggtggcggcc gctctagaac tagtggatcc
      481 cccgggctgc aggaattcga tatcaagctt atcgatgata agctgtcaaa catgagaatt
      541 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
      601 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      661 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      721 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      781 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      841 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      901 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      961 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     1021 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1081 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1141 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1201 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1261 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1321 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1381 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1441 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1501 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1561 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1621 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1681 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1741 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1801 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1861 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1921 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1981 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2041 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2101 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2161 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2221 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2281 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2341 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc g
//
