LOCUS       sequence-230202-        2695 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre-conditional expression of the SthK channel.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Bernal Sierra YA, Rost BR, Pofahl M, Fernandes AM, Kopton RA, Moser 
            S, Holtkamp D, Masala N, Beed P, Tukker JJ, Oldani S, Bonigk W, Kohl
            P, Baier H, Schneider-Warme F, Hegemann P, Beck H, Seifert R, 
            Schmitz D
  TITLE     Potassium channel-based optogenetic silencing.
  JOURNAL   Nat Commun. 2018 Nov 5;9(1):4611. doi: 10.1038/s41467-018-07038-8.
  PUBMED    30397200
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-11-5"; volume: "9(1):4611. doi"; pages: " 
            10.1038/s41467-018-07038-8"
COMMENT     Sequence Label: pAAV-Syn-FLEX-Sthk-P2A-bPAC_minWPRE
FEATURES             Location/Qualifiers
     source          1..2695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      166..621
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(253..272)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     463..484
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(638..657)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     757..779
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(817..835)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        903..1007
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1008..1076,1077..1868)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1226..1245)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2039..2627
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2528..2547
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgctcgctc gctcactgag gccgcccggg cgtcgggcga cctttggtcg cccggcctca
       61 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
      121 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
      181 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
      241 ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
      301 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
      361 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
      421 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
      481 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga
      541 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
      601 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
      661 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      721 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      781 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      841 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      901 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      961 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     1021 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     1081 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     1141 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     1201 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     1261 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     1321 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     1381 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     1441 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     1501 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1561 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1621 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1681 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1741 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1801 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1861 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1921 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1981 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2041 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2101 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2161 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2221 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2281 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2341 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2401 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2461 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2521 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2581 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2641 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtcc tgcag
//
