LOCUS       sequence-230215-        8550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of ZNF787.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Kutsche LK, Gysi DM, Fallmann J, Lenk K, Petri R, Swiersy A, Klapper
            SD, Pircs K, Khattak S, Stadler PF, Jakobsson J, Nowick K, Busskamp 
            V
  TITLE     Combined Experimental and System-Level Analyses Reveal the Complex 
            Regulatory Network of miR-124 during Human Neurogenesis.
  JOURNAL   Cell Syst. 2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011.
  PUBMED    30292704
REFERENCE   2  (bases 1 to 8550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Sep 28. pii: S2405-4712(18)30358-2. doi: 
            10.1016/j.cels.2018.08.011."
COMMENT     Sequence Label: PB09-TRE-ZNF787_G379A
FEATURES             Location/Qualifiers
     source          1..8550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    198..216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    234..252
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    270..288
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    306..324
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    342..360
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    378..396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    414..432
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     477..501
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1738..1779
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1796..1813
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1870..1887)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1876..2083
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        2160..2371
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2384..2652
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2686..3429
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             3430..3483
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3490..3888
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    3930..4051
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3967..3986)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4021..4040
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4813..4847
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5338..5354)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5338..5354)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5351..5373)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5362..5378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5386..5392,5393..5410,5411..5416))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5431..5452
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5569..5586)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5740..6328)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5820..5839)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6499..7290,7291..7359))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7122..7141
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7360..7464)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7490..7945)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7627..7648)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7839..7858
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8072..8094
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8086..8103
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8087..8103
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8233..8295
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc
       61 cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga
      121 gcctgcagac acctgggggg atacggggaa aaggcctcca cggccactag tctttcgtct
      181 tcaagaattc cgtttactcc ctatcagtga tagagaacgt atgaagagtt tactccctat
      241 cagtgataga gaacgtatgc agactttact ccctatcagt gatagagaac gtataaggag
      301 tttactccct atcagtgata gagaacgtat gaccagttta ctccctatca gtgatagaga
      361 acgtatctac agtttactcc ctatcagtga tagagaacgt atatccagtt tactccctat
      421 cagtgataga gaacgtataa gctttaggcg tgtacggtgg gcgcctataa aagcagagct
      481 cgtttagtga accgtcagat cgcctggagc aattccacaa cacttttgtc ttataccaac
      541 tttccgtacc acttcctacc ctcgtaaagg tctagagcta gcgccacatg gagctgcggg
      601 aagaagcctg gtctccgggg ccgctggatt ctgaggacca gcagatggcc agtcacgaga
      661 acccagtgga catcctcatc atggatgatg acgacgtccc cagctggcct cccaccaagc
      721 tgtccccgcc ccagtctgcg cccccagccg gcccgcctcc ccggccgcgg ccgcccgccc
      781 cctacatctg caacgagtgt ggcaagagct ttagccactg gtccaagctg acgcggcacc
      841 agcgcacgca caccggcgag cggcccaacg cctgcgccga ctgcggcaag accttctcgc
      901 agagctcgca cctggtgcag caccggcgca tccacacggg cgagaagccc tacgcctgct
      961 tggagtgcgg caagcgcttc agctggagct ccaacctcat gcagcaccag cgcatccaca
     1021 cgggcgagaa gccctacacc tgccccgact gcggccgcag cttcactcag agcaagagcc
     1081 tggccaagca ccggcgctcg cacagcggcc tcaagccctt cgtgtgcccg cgctgcggcc
     1141 gcggcttcag ccagcccaag agcctcgcgc gtcacctgcg gctgcacccg gagctgtcgg
     1201 ggcctggcgt ggcggccaag gtgctggcgg ctagcgtccg gcgggccaag gggcccgaag
     1261 aggcggtggc ggcggacggc gagatcgcca tccccgtggg cgatggcgag ggcatcatcg
     1321 tggtgggcgc gccgggcgag ggggccgcgg ccgcggcagc catggcgggc gcgggggcaa
     1381 aggccgcggg gccccggtcg cggcgcgccc cggcccccaa gccgtacgtg tgtctggagt
     1441 gcgggaaggg cttcgggcac ggggccgggc tcctggcgca ccagcgggcc cagcacgggg
     1501 acgggctcgg ggcggcgggg ggcgaggagc cggcccacat ctgcgtggag tgcggggagg
     1561 gcttcgtgca gggcgccgcg ctccggagac acaagaagat ccacgcggtg ggcgcgccct
     1621 cggtctgcag cagctgcgga cagagctact accgcgcggg cggggaggag gaggacgacg
     1681 acgacgaggc cgcgggcggg cggtgccccg agtgccgcgc tggggagggc cggtaggggt
     1741 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac cggtccacca
     1801 ccaccaccac cactaaggat ccggggttgg ggttgcgcct tttccaagca cctgctacat
     1861 agcggtaccc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     1921 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     1981 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     2041 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctcttcga
     2101 atttaaatcg gatccagctg aatctaagtg gatctgcgat cgctccggtg cccgtcagtg
     2161 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     2221 gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     2281 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     2341 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc
     2401 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
     2461 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
     2521 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
     2581 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
     2641 ccgttacaga tctaagctgt gaccggcgcc tactctagag ccgccatgtc tagactggac
     2701 aagagcaaag tcataaactc tgctctggaa ttactcaatg gagtcggtat cgaaggcctg
     2761 acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg
     2821 aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc
     2881 cactcctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca
     2941 taccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca
     3001 acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc
     3061 tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta
     3121 ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct
     3181 atgcccccac ttctgaaaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc
     3241 ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc
     3301 ggcgggccga ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt
     3361 gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg
     3421 ctccccgggg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     3481 cctgaattca tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg
     3541 gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct
     3601 agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca
     3661 gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc
     3721 gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt
     3781 ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca
     3841 gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaatg agtcgacaat
     3901 caacctctgg attacaaaat ttgtgggtta acttgtttat tgcagcttat aatggttaca
     3961 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4021 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gaattgactc aaatgatgtc
     4081 aattagtcta tcagaagcta tctggtctcc cttccggggg acaagacatc cctgtttaat
     4141 atttaaacag cagtgttccc aaactgggtt cttatatccc ttgctctggt caaccaggtt
     4201 gcagggtttc ctgtcctcac aggaacgaag tccctaaaga aacagtggca gccaggttta
     4261 gccccggaat tgactggatt ccttttttag ggcccattgg tatggctttt tccccgtatc
     4321 cccccaggtg tctgcaggct caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt
     4381 gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg gctcggggat gcggggggag
     4441 cgccggaccg gagcggagcc ccgggcggct cgctgctgcc ccctagcggg ggagggacgt
     4501 aattacatcc ctgggggctt tggggggggg ctgtccctga tatctataac aagaaaatat
     4561 atatataata agttatcacg taagtagaac atgaaataac aatataatta tcgtatgagt
     4621 taaatcttaa aagtcacgta aaagataatc atgcgtcatt ttgactcacg cggtcgttat
     4681 agttcaaaat cagtgacact taccgcattg acaagcacgc ctcacgggag ctccaagcgg
     4741 cgactgagat gtcctaaatg cacagcgacg gattcgcgct atttagaaag agagagcaat
     4801 atttcaagaa tgcatgcgtc aattttacgc agactatctt tctagggtta atctagctgc
     4861 atcaggatca tatcgtcggg tcttttttcc ggctcagtca tcgcccaagc tggcgctatc
     4921 tgggcatcgg ggaggaagaa gcccgtgcct tttcccgcga ggttgaagcg gcatggaaag
     4981 agtttgccga ggatgactgc tgctgcattg acgttgagcg aaaacgcacg tttaccatga
     5041 tgattcggga aggtgtggcc atgcacgcct ttaacggtga actgttcgtt caggccacct
     5101 gggataccag ttcgtcgcgg cttttccgga cacagttccg gatggtcagc ccgaagcgca
     5161 tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg tgccggtgtg cagattaatg
     5221 acagcggtgc ggcgctggga tattacgtca gcgaggacgg gtatcctggc tggatgccgc
     5281 agaaatggac atggataccc cgtgagttac ccggcgggcg cgcttggcgt aatcatggtc
     5341 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     5401 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     5461 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5521 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     5581 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5641 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5701 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5761 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5821 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5881 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     5941 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6001 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6061 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6121 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6181 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6241 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6301 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6361 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6421 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6481 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6541 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6601 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6661 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6721 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6781 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6841 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6901 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6961 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7021 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7081 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7141 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7201 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7261 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7321 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7381 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7441 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     7501 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     7561 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     7621 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     7681 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     7741 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     7801 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     7861 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     7921 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
     7981 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     8041 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     8101 agtgagcgcg cctcgttcat tcacgttttt gaacccgtgg aggacgggca gactcgcggt
     8161 gcaaatgtgt tttacagcgt gatggagcag atgaagatgc tcgacacgct gcagaacacg
     8221 cagctagatt aaccctagaa agataatcat attgtgacgt acgttaaaga taatcatgtg
     8281 taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt aatttgaata
     8341 gatattaagt tttattatat ttacacttac atactaataa taaattcaac aaacaattta
     8401 tttatgttta tttatttatt aaaaaaaaca aaaactcaaa atttcttcta taaagtaaca
     8461 aaacttttat gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc
     8521 ccgctagggg gcagcagcga gccgcccggg
//
