LOCUS       sequence-23024-e         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Torrance CJ, Agrawal V, Vogelstein B, Kinzler KW
  TITLE     Use of isogenic human cancer cells for high-throughput screening and
            drug discovery.
  JOURNAL   Nat Biotechnol. 2001 Oct . 19(10):940-5.
  PUBMED    11581659
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2001 Oct . 19(10):940-5."
COMMENT     Sequence Label: IRES-puro-GFP
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        44..62
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(114..116,117..119,120..578)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMA"
     primer_bind     complement(159..180)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(420..439)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tctctggcta ctagagaccc actgcttact ggcttatcga aattaatacg actcactata
       61 gggagaccca agcttggtac cgagctcgga tcgatatctg cggcctagct agcatggtga
      121 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      181 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      241 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      301 ccaccttctc ctacggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
      361 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      421 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      481 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
      541 agtacaactt caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
      601 aggtgaactt
//
