LOCUS       sequence-23025-e         523 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Torrance CJ, Agrawal V, Vogelstein B, Kinzler KW
  TITLE     Use of isogenic human cancer cells for high-throughput screening and
            drug discovery.
  JOURNAL   Nat Biotechnol. 2001 Oct . 19(10):940-5.
  PUBMED    11581659
REFERENCE   2  (bases 1 to 523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2001 Oct . 19(10):940-5."
COMMENT     Sequence Label: IRES-puro-YFP
FEATURES             Location/Qualifiers
     source          1..523
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(144..163)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          146..375
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     complement(453..474)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tatagacaac gcacaccggc cttattccaa gcggcttcgg ccagtaacgt tagggggggg
       61 ggagggagag gggggggggg gagaggggcg gaattggccg ccctagatgc atgctcgacc
      121 tgcagttgga cctgggagtg gacacctgtg gagagaaagg caaagtggat gtcattgtca
      181 ctcaagtgta tggccagatc tcaagcctgc cacacctcaa gcttgacaac aaaaagattg
      241 tcttttctga ccagatggac gcggccaccc tcaaaggcat caccgcgggc caggtgaata
      301 tcaaatcctc ctcgtttttg gaaactgaca atcttagcgc agaagtcatg cccgcttttg
      361 agagggagta ctcaccccaa cagctggccc tcgcagacag cgaattctta cttgtacagc
      421 tggtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
      481 cgcttctcgt tggggtcttt gctcagggcg gactggtagc tca
//
