LOCUS       sequence-23028-e         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Saha S, Bardelli A, Buckhaults P, Velculescu VE, Rago C, St Croix B,
            Romans KE, Choti MA, Lengauer C, Kinzler KW, Vogelstein B
  TITLE     A phosphatase associated with metastasis of colorectal cancer.
  JOURNAL   Science. 2001 Nov 9. 294(5545):1343-6.
  PUBMED    11598267
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2001 Nov 9. 294(5545):1343-6."
COMMENT     Sequence Label: pCEP4-PRL-3
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             72..98
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     primer_bind     complement(677..696)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     731..750
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tcgtttagtg accgtcagat ctctagaagc tgggtaccga aatgggatac gatgttccag
       61 attacgcttc gtacgatgtt ccagattacg caagcttggc tcggatgaac cgcccggccc
      121 cggtggaggt gagctacaaa cacatgcgct tcctcatcac ccacaacccc accaacgcca
      181 cgctcagcac cttcattgag gacctgaaga agtacggggc taccactgtg gtgcgtgtgt
      241 gtgaagtgac ctatgacaaa acgccgctgg agaaggatgg catcaccgtt gtggactggc
      301 cgtttgacga tggggcgccc ccgcccggca aggtagtgga agactggctg agcctggtga
      361 aggccgagtt ctgtgaggcc cccggcagct gcgtggctgt gcactgcgtg gcgggcctgg
      421 gccgggctcc agtccttgtg gcgctggccc ttattgagag cgggatgaag tacgaggacg
      481 ccatccagtt catccgccag aagcgccgcg gagccatcaa cagcaagcag ctcacctacc
      541 tggagaaata ccggcccaaa cagaggctgc ggttcaaaga cccacacacg cacaagaccc
      601 ggtgctgcgt tatgtagctc aggaccgcgg ccgctcgagg ccggcaaggc cggatccaga
      661 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      721 ctttatttgt gaaatttgtg atgctattgc tt
//
