LOCUS       sequence-230284-        9676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression in cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Hsu AY, Wang D, Liu S, Lu J, Syahirah R, Bennin DA, Huttenlocher A, 
            Umulis DM, Wan J, Deng Q
  TITLE     Phenotypical microRNA screen reveals a noncanonical role of CDK2 in 
            regulating neutrophil migration.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116.
  PUBMED    31451657
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 
            10.1073/pnas.1905221116."
COMMENT     Sequence Label: hsa-miR 19b plix 4.03
FEATURES             Location/Qualifiers
     source          1..9676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             8..25
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        35..545
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     464..482
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             555..1151
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(555..574)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1051..1071
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1152..1205
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1206..1823,1824..1829,1830..1865,1866..1868,1869..1904,
                     1905..1907,1908..1943,1944..1952)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(1992..2012)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2410..2421)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2626..2859
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2931..3052
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2968..2987)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3022..3041
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3092..3227
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3227..3298
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3299..3367,3368..4159)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3517..3536)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4330..4918
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4819..4838
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5072..5089
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5206..5227
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5242..5247,5248..5265,5266..5272)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5280..5296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5285..5307
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5304..5320
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5304..5320
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5339..5359
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5341..5359
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5387..5613
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5614..5794
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5841..5966
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6459..6692
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6877..6921
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7134..7251
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7305..7619
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    7313..7331
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7349..7367
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7384..7402
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7420..7438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7456..7474
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7491..7509
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7527..7545
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     7592..7616
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      7636..7645
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8985..9676,1)
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
ORIGIN
        1 accggtccac caccaccacc accactaagg atccggggtt ggggttgcgc cttttccaag
       61 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
      121 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
      181 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
      241 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc
      301 tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca
      361 atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg
      421 cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca
      481 agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc
      541 cccagcaatt caccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
      601 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
      661 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
      721 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
      781 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
      841 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
      901 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
      961 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     1021 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     1081 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     1141 agcccggtgc cgaaggtaga ggttctctcc tcacttgtgg tgatgttgaa gaaaaccctg
     1201 gtccaatgtc tagactggac aagagcaaag tcataaacgg agctctggaa ttactcaatg
     1261 gtgtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc
     1321 ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga
     1381 tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc
     1441 tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac ggggctaaag
     1501 tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt
     1561 tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc
     1621 actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag
     1681 agacacctac caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc
     1741 ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga
     1801 aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt gacttagaca
     1861 tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg
     1921 acgattttga ccttgacatg ctccccgggt aactaagtaa ggatcgatcc aagatatcgt
     1981 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2041 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     2101 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     2161 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     2221 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     2281 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     2341 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     2401 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     2461 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     2521 tccccgcctg tttcgcctcg gcgtccggac tagaggtacc tttaagacca atgacttaca
     2581 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     2641 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     2701 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     2761 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     2821 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     2881 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     2941 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3001 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3061 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3121 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3181 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggccttt caaatatgta
     3241 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     3301 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     3361 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     3421 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     3481 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3541 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3601 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3661 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3721 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3781 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3841 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3901 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3961 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     4021 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     4081 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     4141 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     4201 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     4261 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4321 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4381 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4441 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4501 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4561 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4621 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4681 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4741 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4801 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4861 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4921 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4981 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     5041 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     5101 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     5161 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     5221 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     5281 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
     5341 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
     5401 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
     5461 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
     5521 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
     5581 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
     5641 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     5701 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     5761 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     5821 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     5881 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     5941 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     6001 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     6061 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     6121 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     6181 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     6241 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     6301 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     6361 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     6421 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     6481 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     6541 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     6601 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     6661 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     6721 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     6781 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     6841 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     6901 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     6961 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     7021 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     7081 accccgaggg gacaattctc gacctcgaga caaatggcag tattcatcca caattttaaa
     7141 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     7201 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     7261 tacagggaca gcagagatcc actttggccg cgaatcgata tgtcgagttt actccctatc
     7321 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg atgtcgagtt
     7381 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     7441 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag
     7501 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta
     7561 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     7621 ggagaattgg ctagcgccac catggatgag gaaatcgctg ccctggtcgt tgacaacggc
     7681 tccggtatgt gcaaagccgg ttttgctgga gatgatgccc ctcgtgctgt tttcccctcc
     7741 attgttggac gacccagaca tcaggtacat ttaataaacc atcctaataa catgttaatc
     7801 atattgaacc acattaaact ctcatttgtg cattttacat cttttaaaac cgatttatat
     7861 tcgtttttag ataatgaagt ttattaaagt gttaattcac agctttattg cctgttttga
     7921 gaatctgctt gcataagtga tttttaaact tgatctgatt ctcgtttgtt tcgaagttct
     7981 tcagctttga aagtcagatc ttcattcaca gccatgccat agttttagga tacagtataa
     8041 gtagacgaat tgggatgcat cgtagatttt atgctgtgtt tgttatggtt tttcagtggt
     8101 agagattttc agagtattat tagggttata gtcagtgtca ggtttattta tcgtcagcgt
     8161 gatgggcaaa aaacagctgg cagtacgggc tgcctgtcgc ccaatcaaac tgtcctgtta
     8221 ctgaacactg ttctatggtt agttttgcag gtttgcatcc agctgtgtga tattctgctg
     8281 tgcaaatcca tgcaaaactg actgtggtag tgaaaagtct gtagaaaagt aagggaaact
     8341 caaacccctt tctacacagg ttgggatcgg ttgcaatgct gtgtttctgt atggtattgc
     8401 acttgtcccg gcctgttgag tttggtgggg attgtgacca gaagattttg aaaattaaat
     8461 attactgaag atttcgactt ccactgttaa atgtacaaga tacatgaaat attaaagaaa
     8521 atgtgtaact ttttgtgtaa atacatcttg tcttgttttc attcaaaaac atttcacttt
     8581 tggggttgcg tgagccaact gctgttactc tcgcccagaa atctgcgcca acatggacac
     8641 acccagtgga gcaagccatc tgattttcat ggtcagattg taaagaagca attactcttg
     8701 gacagtttaa gcagaaaagc caccaagtca agtcactggt catatgatga actcgaagat
     8761 ctaataaaag ttaatttaag cgttcattac aataaattca agtgaatctg ttttaagcta
     8821 gcctttaacg tttttaaatt ccttcaaaca aattggatcc tctttgcagt tcctcatttc
     8881 tcggtgagct ggtgctttaa tccctttact ttttcttcct ctagggagtg atggttggca
     8941 tgggacgaat taattcctgc agcccggggg taccggtcgc caccatgaac accccgggaa
     9001 ttaacctgat caaggaggac atgcgcgtga aggtgcacat ggagggcaac gtgaacggcc
     9061 acgccttcgt gatcgagggc gagggcaagg gcaagcccta cgagggcacc cagaccgcca
     9121 acctgaccgt gaaggagggc gcccccctgc ccttcagcta cgacatcctg accaccgccg
     9181 tgcactacgg caaccgggtg ttcaccaagt accccgagga catccccgac tacttcaagc
     9241 agagcttccc cgagggctac agctgggagc gcaccatgac cttcgaggac aagggcatct
     9301 gcaccatccg cagcgacatc agcctggagg gcgactgctt cttccagaac gtgcgcttca
     9361 agggcaccaa cttccccccc aacggccccg tgatgcagaa gaaaaccctg aagtgggagc
     9421 ccagcaccga gaagctgcac gtgcgcgacg gcctgctggt gggcaacatc aacatggccc
     9481 tgctgctgga gggcggcggc cactacctgt gcgacttcaa gaccacctac aaggccaaga
     9541 aggtggtgca gctgcccgac gcccacttcg tggaccaccg catcgagatc ctgggcaacg
     9601 acagcgacta caacaaggtg aagctgtacg agcacgccgt ggcccgctac agccccctgc
     9661 ccagccaggt gtggta
//
