LOCUS       sequence-230317-       10710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10710)
  AUTHORS   Raj B, O'Hanlon D, Vessey JP, Pan Q, Ray D, Buckley NJ, Miller FD, 
            Blencowe BJ
  TITLE     Cross-regulation between an alternative splicing activator and a 
            transcription repressor controls neurogenesis.
  JOURNAL   Mol Cell. 2011 Sep 2;43(5):843-50.
  PUBMED    21884984
REFERENCE   2  (bases 1 to 10710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-09-2"; volume: "43(5)"; pages: "843-50"
COMMENT     Sequence Label: pLDPuro-hsnSR100N
FEATURES             Location/Qualifiers
     source          1..10710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..63
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    180..201
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(216..221,222..239,240..246)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    254..270
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     259..281
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     278..294
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     278..294
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     313..333
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        315..333
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        361..587
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             588..768
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    815..940
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1433..1666
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1851..1895
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2193..2213
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2633..2651
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2668..2982
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2676..2694
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2712..2730
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2747..2765
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2783..2801
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2819..2837
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2854..2872
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2890..2908
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2955..2979
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3032..3056
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3105..3128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(4962..4986)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5066..5089
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5126..5149
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5162..5179
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5189..5209
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5222..5242
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5255..5320
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5368..5485
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5534..6044
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5963..5981
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(6056..6075)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6552..6572
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6692..6757
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             join(6764..7381,7382..7387,7388..7423,7424..7426,7427..7462,
                     7463..7465,7466..7501,7502..7510)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     LTR             7589..7822
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7894..8015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7931..7950)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7985..8004
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8055..8190
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8210..8229)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8211..8229)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8239..8256)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8239..8255)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8248..8270)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8397..8852
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8484..8503)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8694..8715
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8878..8982
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8983..9051,9052..9843)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9201..9220)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10014..10602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10503..10522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
       61 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      121 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
      181 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      241 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
      301 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
      361 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
      421 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      481 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      541 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      601 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      661 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      721 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      781 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      841 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      901 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      961 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     1021 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     1081 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     1141 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     1201 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1261 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1321 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1381 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1441 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1501 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1561 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1621 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1681 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1741 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1801 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1861 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1921 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1981 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     2041 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     2101 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     2161 gagactagcc tcgagcggcc gcccccttca ccgagggcct atttcccatg attccttcat
     2221 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
     2281 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     2341 ttttaaaatt atgttttaaa atggactatc atatgccaag tacgccccct attgacgtca
     2401 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     2461 cttggcagta catctacatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2521 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2581 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     2641 tatcacgagg ccctttcgtc ttcactcgag tttactccct atcagtgata gagaacgtat
     2701 gtcgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc tatcagtgat
     2761 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
     2821 cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga gaacgtatgt
     2881 cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt acggtgggag
     2941 gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagaat tcgagctcgg
     3001 tacccgggga tcctctagtc agctgacgcg tacaagtttg tacaaaaaag caggctacca
     3061 tggactacaa agaccatgac ggtgatacat atgatcatga catcgattac aaggatgacg
     3121 atgacaaggc gagcgttcag caaggcgaga agcagctttt tgagaagttc tggcgaggaa
     3181 ccttcaaagc ggtggccacc ccccgtcccg agagcatcat tgtcgccagt atcacggccc
     3241 gcaagccgct gccaaggaca gagccccaga ataaccccgt tgtcccagct caggatggac
     3301 cctcagaaaa gctgggtcag catctggcca ccgagccctt gggcaccaac agttgggaga
     3361 gagacaagac ctgtcgggaa ctgggtgcca ccagaggaca cagtgcctct catgacaaag
     3421 acttgacacc accaccttcc tccaggggaa agaagaaaaa gaagaaatcc actcggaaga
     3481 agagaaggag gtcctcatcc tatagcccat cgcctgtcaa gaaaaagaag aagaaaagtt
     3541 ccaagaaaca caagcgacgc aggtcattct ccaagaagag aaggcacagc tcctctagcc
     3601 caaaaagcaa aagaagggat gagaagaggc acaagaaaca atctcgaagc cggccccgaa
     3661 agtctcaccg ccaccgccat caccgctgcc cctcgcggtc ccagagctcg gagtcccgcc
     3721 cctcaagctg tgagagcagg caccgcggcc ggtcccctga ggaagggcag aagtcccgcc
     3781 gaaggcactc ccgccgctgc tccaagaccc tctgcaagga cagccctgag gcccagtcca
     3841 gtcgcccgcc cagtcaaccc ctccagatgc ttggctacct gtcagccagg ggtgtaatca
     3901 ctgggtcggg gtctgctgct gacctcttta ccaaaacagc cagcccgctc accacctcgc
     3961 gaggacgttc ccaggagtac gactcaggaa atgacacgtc ctcgccaccc tccacgcaaa
     4021 ccagctcagc caggtctcgg ggccaggaga aggggagccc cagtgggggc ttgagcaaga
     4081 gccgggagct caacagtggc aacacctctg attcagggaa ctccttcacc acctcctcac
     4141 cccagaacaa gggggccatg ttggagaatc tctcccccac cagcaggggc agagagtcaa
     4201 ggggatttca gtcaccgtgt ctggaatgtg ccgaagtgaa gaagtccagt ttggtcccat
     4261 ccacagcccg gagctcaccc atgaaagggt gttcccgcag ctcctcctat gccagcaccc
     4321 gatcctccag tcactcgtcc cgatccccaa atcccagggc ttcccccagg tacacccaaa
     4381 gccgatccac ctcttctgaa aaaaggtcct actcccgctc tcccagctat tcctccaagt
     4441 ctggcaagag gagcccgccc agcagaagct ctaggtcccg ccgcagccct agctactccc
     4501 gctacagccc cagcagggag cgggatccca aatacagtga gaaggactca cagcagcggg
     4561 agcgcgagcg agcgcgtcgg agacgtcggt cctactcgcc tatgagaaag cgccggagag
     4621 actccccgag ccacctggag gcccggagga taaccagtgc ccggaaacgc cccatcccct
     4681 actatcggcc cagcccctcc tcatccggca gcctcagcag cacctcctcc tggtacagca
     4741 gcagcagtag ccgctcggcc agccgcagtt actcccggag ccggagtcgg agccggagcc
     4801 ggagacggag ccggacccgc acgagcagca gctctagctc ccgcagccct agtccgggct
     4861 cccgcagccg gagccggagc aggagccgga gccggagccg gagcaggagc cagagccgga
     4921 gctacagctc agcagacagc tactccagca cgaggcgcta aacccagctt tcttgtacaa
     4981 agtggttgat tctagagcga tcgccagaga gctgttcaac ctgctgggcg agaaccagcc
     5041 ccccgtggtg atcaaggcga tcgcctggag ccaccctcag ttcgagaagg gaggaggaag
     5101 cggcggaggc agcggaggag gaagctggag ccacccgcag ttcgagaaag gcggaagcgg
     5161 ccatcaccat caccatcacc ctgcaggaga aaatctgtac ttccagggga gaggaggcag
     5221 cgagaacctg tactttcagg ggcctgcagg aagagactac aaagaccatg acggtgatta
     5281 taaagatcat gacatcgatt acaaggatga cgatgacaag tagttcgaat tctcgacctc
     5341 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     5401 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     5461 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     5521 gccgcggctc gagggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg
     5581 gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca
     5641 cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc
     5701 cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc
     5761 ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg
     5821 gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc
     5881 gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt
     5941 gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg
     6001 cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggat ccaccatgac
     6061 cgagtacaag cccaccgtgc gcctcgccac ccgcgacgac gtgcccaggg ccgtccgcac
     6121 cctcgccgcc gctttcgccg actaccccgc cacccgccac accgtcgatc ccgaccgcca
     6181 catcgagcgg gtcaccgagc tgcaagaact cttcctcacc cgcgtcgggc tcgacatcgg
     6241 caaggtgtgg gtcgctgacg acggcgccgc agtggctgtc tggaccacac ccgagagcgt
     6301 cgaagctggg gcggtgttcg ccgagatcgg ccctcgcatg gccgagttga gcggttcccg
     6361 gctggccgct cagcaacaga tggaaggcct cctggctcca caccggccca aggagcccgc
     6421 atggttcctg gccaccgtcg gcgtctctcc cgaccaccag ggcaagggtc tgggcagcgc
     6481 cgtcgtgctc cccggagtgg aggctgccga gcgcgccggg gtgcccgcct tcctggagac
     6541 ctccgcaccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     6601 ggaggtgccc gaaggaccac gcacctggtg catgacccgc aagcccggtg ccctgcaggg
     6661 atctggcgaa gccagacaca aacagaaaat tgtgaaacag actttgaatt ttgaccttct
     6721 caagttggcg ggagacgtgg agtccaaccc agggcccgct agcatgtcta gactggacaa
     6781 gagcaaagtc ataaacggag ctctggaatt actcaatggt gtcggtatcg aaggcctgac
     6841 gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa
     6901 gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca
     6961 cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata
     7021 ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac
     7081 agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc
     7141 cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt
     7201 ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat
     7261 gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt
     7321 ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg
     7381 cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga
     7441 cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct
     7501 ccccgggtaa atgcatcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7561 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     7621 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7681 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7741 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7801 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     7861 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     7921 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7981 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     8041 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     8101 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     8161 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     8221 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     8281 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     8341 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     8401 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8461 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8521 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8581 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8641 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8701 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     8761 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     8821 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     8881 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8941 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     9001 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     9061 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     9121 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     9181 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     9241 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     9301 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     9361 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     9421 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     9481 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     9541 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     9601 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     9661 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     9721 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     9781 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     9841 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9901 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9961 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    10021 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    10081 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    10141 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    10201 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    10261 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    10321 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    10381 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    10441 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    10501 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    10561 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    10621 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    10681 cctgattctg tggataaccg tattaccgcc
//
