LOCUS       sequence-23033-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Samuels Y, Wang Z, Bardelli A, Silliman N, Ptak J, Szabo S, Yan H, 
            Gazdar A, Powell SM, Riggins GJ, Willson JK, Markowitz S, Kinzler 
            KW, Vogelstein B, Velculescu VE
  TITLE     High frequency of mutations of the PIK3CA gene in human cancers.
  JOURNAL   Science. 2004 Apr 23. 304(5670):554.
  PUBMED    15016963
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2004 Apr 23. 304(5670):554."
COMMENT     Sequence Label: PIK3CA - Exon20 H1047R
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        39..57
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      92..101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..124
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgaaccgtca gatccgctag cgattacgcc aagctcgaaa ttaaccctca ctaaagggaa
       61 caaaagctgg agctccaccg cggtggcggc cgccaccatg gattacaagg atgacgacga
      121 taaggcccgg gcggatcccc cgggctgcag gaattcgata gttcgaacca tgcctccaag
      181 accatcatca ggtgaactgt ggggcatcca cttgatgccc ccaagaatcc tagtagaatg
      241 tttactacca aatggaatga tagtgacttt agaatgcctc cgcgaggcta cattaataac
      301 cataaagcat gaactattta aagaagcaag aaaatacccc ctccatcaac ttcttcaaga
      361 tgaatcttct tacattttcg taagtgttac tcaagaagca gaaagggaag aattttttga
      421 tgaaacaaga cgactttgtg accttcggct ttttcaaccc tttttaaaag taattgaacc
      481 agtaggcaac cgtgaagaaa agatcctcga tcgagaaatt ggttttgcta tcggcatgcc
      541 agtgtgtgaa tttgatatgg ttaaagatcc agaagtacag gacttccgaa gaaatattct
      601 gaacgtttgt aaagaagctg tggatcttag ggacctcaat tcacctcata gtagagcaat
      661 gtatgtctat cctccaaatg tagaatcttc accagaatcg ccaaagcaca tatacnataa
      721 attagataaa gggcaaataa tagtggtgat ctgggtaata gtttctccaa ataatgacaa
      781 gcagaagtat actctgaaaa tcaaccatga ctgtgtacca gaacaagtaa ttgctgaagc
      841 aatcaggaaa aaaactcgaa gtag
//
