LOCUS       sequence-230341-        5618 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UAS-driven expresion of Voltron in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Abdelfattah AS, Kawashima T, Singh A, Novak O, Liu H, Shuai Y, Huang
            YC, Campagnola L, Seeman SC, Yu J, Zheng J, Grimm JB, Patel R, 
            Friedrich J, Mensh BD, Paninski L, Macklin JJ, Murphy GJ, Podgorski 
            K, Lin BJ, Chen TW, Turner GC, Liu Z, Koyama M, Svoboda K, Ahrens 
            MB, Lavis LD, Schreiter ER
  TITLE     Bright and photostable chemigenetic indicators for extended in vivo 
            voltage imaging.
  JOURNAL   Science. 2019 Aug 1. pii: science.aav6416. doi: 
            10.1126/science.aav6416.
  PUBMED    31371562
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2019 Aug 1. pii: science.aav6416. doi: 10.1126/science.aav6416."
COMMENT     Sequence Label: pTol2-10xUAS-Voltron
FEATURES             Location/Qualifiers
     source          1..5618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1806..1940
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1830..1849)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1884..1903
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2348..2535,2536..2547)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2581..2597)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2581..2597)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(2634..2652)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2673..2689)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2673..2689)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2686..2708)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2697..2713
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2721..2727,2728..2745,2746..2751))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(2904..2921)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3075..3663)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3155..3174)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3834..4625,4626..4694))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4457..4476
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_feature    join(4725..4736,4737..4899)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        5343..5361
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cggagtactg tcctccgcca tacgtagcag ggtagactgt agagggtata taatggaacc
       61 catcgcgtct cagcctcact ttgagctcct ccacacgaat tcgccatcat ggctgacgtg
      121 gaaaccgaga ccggcatgat tgcacagtgg attgtctttg ctattatggc tgctgctgct
      181 attgcttttg gagtggctgt gcactttcgg ccttcagagc tgaagagcgc atactatatc
      241 aacattgcca tctgcactat cgccgctacc gcttactatg caatggccgt gaactaccag
      301 gacctgacaa tgaatggtga aaggcaggtg gtctacgcaa gatatattaa ctgggtgctg
      361 accacaccac tgctcctgct cgatctcatc gtcatgacca agatgggcgg agtgatgatt
      421 tcttgggtca tcggcgcaga cattttcatg atcgtgtttg gtattctggg cgccttcgag
      481 gatgaacaca agttcaaatg ggtgtacttt atcgctggat gtgtgatgca ggcagtcctg
      541 acatacggga tgtataacgc cacttggaaa gacgatctga agaaaagccc cgagtaccat
      601 agctcctatg tcagtctgct cgtcttcctg tcaatcctct gggtgtttta tcctgtcgtg
      661 tgggctttcg ggtctggtag tggcgtgctg tccgtcgaca atgaggccat tctcatggga
      721 atcctggatg tgctcgctaa gccactgttt ggaatggggt gcctcattgc ccatgagact
      781 atcttcaaga tcggtactgg ctttccattc gacccccatt atgtggaagt cctgggcgag
      841 cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc ctgtgctgtt cctgcacggt
      901 aacccgacct cctcctacgt gtggcgcaac atcatcccgc atgttgcacc gacccatcgc
      961 tgcattgctc cagacctgat cggtatgggc aaatccgaca aaccagacct gggttatttc
     1021 ttcgacgacc acgtccgctt catggatgcc ttcatcgaag ccctgggtct ggaagaggtc
     1081 gtcctggtca ttcacgactg gggctccgct ctgggtttcc actgggccaa gcgcaatcca
     1141 gagcgcgtca aaggtattgc atttatggag ttcatccgcc ctatcccgac ctgggacgaa
     1201 tggccagaat ttgcccgcga gaccttccag gccttccgca ccaccgacgt cggccgcaag
     1261 ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc cgatgggtgt cgtccgcccg
     1321 ctgactgaag tcgagatgga ccattaccgc gagccgttcc tgaatcctgt tgaccgcgag
     1381 ccactgtggc gcttcccaaa cgagctgcca atcgccggtg agccagcgaa catcgtcgcg
     1441 ctggtcgaag aatacatgga ctggctgcac cagtcccctg tcccgaagct gctgttctgg
     1501 ggcaccccag gcgttctgat cccaccggcc gaagccgctc gcctggccaa aagcctgcct
     1561 aactgcaagg ctgtggacat cggcccgggt ctgaatctgc tgcaagaaga caacccggac
     1621 ctgatcggca gcgagatcgc gcgctggctg tcgacgctcg agatttccgg cgagccaacc
     1681 actaagagca ggatcaccag cgagggcgag tacatccccc tggaccagat cgacatcaac
     1741 gtgttctgct acgagaacga ggtgtaaact cgagcctcta gaactatagt gagtcgtatt
     1801 acgtagatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     1861 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     1921 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     1981 gggaggtgtg ggaggttttt taattcgcgg ccgcggatcc agatcgatct gcgaagatac
     2041 ggccacgggt gctcttgatc ctgtggctga ttttggactg tgctgctcgc agctgctgat
     2101 gaatcacata cttcctccat tttcttccac tgattgactg ttataatttc cctaatttcc
     2161 aggtcaaggt gctgtgcatt gtggtaatag atgtgacatg acgtcacttc caaaggacca
     2221 atgaacatgt ctgaccaatt tcatataatg tgaaaacgat tttcataggc agaataaata
     2281 acatttaaat taaactgggc atcagcgcaa ttcaattggt ttggtaatag caagggaaaa
     2341 tagaatgaag tgatctccaa aaaataagta ctttttgact gtaaataaaa ttgtaaggag
     2401 taaaaagtac ttttttttct aaaaaaatgt aattaagtaa aagtaaaagt attgattttt
     2461 aattgtactc aagtaaagta aaaatcccca aaaataatac ttaagtacag taatcaagta
     2521 aaattactca agtactttac acctctggtt cttgaccccc taccttcagc aagcccagca
     2581 gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag
     2641 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     2701 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     2761 gcctaatgag tgagccaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     2821 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     2881 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     2941 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3001 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3061 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3121 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3181 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3241 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3301 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3361 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3421 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3481 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3541 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3601 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3661 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3721 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3781 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3841 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3901 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3961 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4021 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4081 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     4141 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     4201 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     4261 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     4321 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     4381 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4441 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4501 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4561 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4621 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4681 tgttgaatac tcatactctt ccggtaccgg catatggttc ttgacagagg tgtaaaaagt
     4741 actcaaaaat tttactcaag tgaaagtaca agtacttagg gaaaatttta ctcaattaaa
     4801 agtaaaagta tctggctaga atcttacttg agtaaaagta aaaaagtact ccattaaaat
     4861 tgtacttgag tattaaggaa gtaaaagtaa aagcaagaaa gaaaactaga gattcttgtt
     4921 taagctttta atctcaaaaa acattaaatg aaatgcatac aaggttttat cctgctttag
     4981 aactgtttgt atttaattat caaactataa gacagacaat ctaatgccag tacacgctac
     5041 tcaaagttgt aaaacctcag atttaacttc agtagaagct gattctcaaa attgttagtg
     5101 tcaagcctag ctcttttggg gctgaaaagc aatcctgcag tgctgaaaag cctctcacag
     5161 gcagccgatg cgggaagagg tgtattagtc ttgatagaga ggctgcaaat agcaggaaac
     5221 gtgagcagag actccctggt gtctgaaaca caggccagat gggccctcga cggtatcgat
     5281 aagaaaacta gagattcttg tttaagcttg aggctgctgt cagggagctc atggacttcg
     5341 agaattaacc ctcactaaag ggaatacgaa gttatccgga gtactgtcct ccgacactag
     5401 aggtcggagt actgtcctcc gacgcaaggc ggagtactgt cctccgacgc aaggtcggag
     5461 tactgtcctc cgacactaga ggtcggagta ctgtcctccg ccatacttat ccggagtact
     5521 gtcctccgac actagaggtc ggagtactgt cctccgacgc aaggcggagt actgtcctcc
     5581 gacgcaaggt cggagtactg tcctccgaca ctagaggt
//
