LOCUS       sequence-230343-        5813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UAS-driven expresion of soma-localized Voltron in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Abdelfattah AS, Kawashima T, Singh A, Novak O, Liu H, Shuai Y, Huang
            YC, Campagnola L, Seeman SC, Yu J, Zheng J, Grimm JB, Patel R, 
            Friedrich J, Mensh BD, Paninski L, Macklin JJ, Murphy GJ, Podgorski 
            K, Lin BJ, Chen TW, Turner GC, Liu Z, Koyama M, Svoboda K, Ahrens 
            MB, Lavis LD, Schreiter ER
  TITLE     Bright and photostable chemigenetic indicators for extended in vivo 
            voltage imaging.
  JOURNAL   Science. 2019 Aug 1. pii: science.aav6416. doi: 
            10.1126/science.aav6416.
  PUBMED    31371562
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2019 Aug 1. pii: science.aav6416. doi: 10.1126/science.aav6416."
COMMENT     Sequence Label: pTol2-10xUAS-Voltron-ST
FEATURES             Location/Qualifiers
     source          1..5813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1926..2060
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1950..1969)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2004..2023
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2468..2655,2656..2667)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2701..2717)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2701..2717)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(2754..2772)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2793..2809)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2793..2809)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2806..2828)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2817..2833
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2841..2847,2848..2865,2866..2871))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(3024..3041)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3195..3783)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3275..3294)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3954..4745,4746..4814))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4577..4596
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_feature    join(4845..4856,4857..5019)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        5463..5481
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcactttgag ctcctccaca cgaattcgcc atcatggctg acgtggaaac cgagaccggc
       61 atgattgcac agtggattgt ctttgctatt atggctgctg ctgctattgc ttttggagtg
      121 gctgtgcact ttcggccttc agagctgaag agcgcatact atatcaacat tgccatctgc
      181 actatcgccg ctaccgctta ctatgcaatg gccgtgaact accaggacct gacaatgaat
      241 ggtgaaaggc aggtggtcta cgcaagatat attaactggg tgctgaccac accactgctc
      301 ctgctcgatc tcatcgtcat gaccaagatg ggcggagtga tgatttcttg ggtcatcggc
      361 gcagacattt tcatgatcgt gtttggtatt ctgggcgcct tcgaggatga acacaagttc
      421 aaatgggtgt actttatcgc tggatgtgtg atgcaggcag tcctgacata cgggatgtat
      481 aacgccactt ggaaagacga tctgaagaaa agccccgagt accatagctc ctatgtcagt
      541 ctgctcgtct tcctgtcaat cctctgggtg ttttatcctg tcgtgtgggc tttcgggtct
      601 ggtagtggcg tgctgtccgt cgacaatgag gccattctca tgggaatcct ggatgtgctc
      661 gctaagccac tgtttggaat ggggtgcctc attgcccatg agactatctt caagatcggt
      721 actggctttc cattcgaccc ccattatgtg gaagtcctgg gcgagcgcat gcactacgtc
      781 gatgttggtc cgcgcgatgg cacccctgtg ctgttcctgc acggtaaccc gacctcctcc
      841 tacgtgtggc gcaacatcat cccgcatgtt gcaccgaccc atcgctgcat tgctccagac
      901 ctgatcggta tgggcaaatc cgacaaacca gacctgggtt atttcttcga cgaccacgtc
      961 cgcttcatgg atgccttcat cgaagccctg ggtctggaag aggtcgtcct ggtcattcac
     1021 gactggggct ccgctctggg tttccactgg gccaagcgca atccagagcg cgtcaaaggt
     1081 attgcattta tggagttcat ccaccctatc ccgacctggg acgaatggcc agaatttgcc
     1141 cgcgagacct tccaggcctt ccgcaccacc gacgtcggcc gcaagctgat catcgatcag
     1201 aacgttttta tcgagggtac gctgccgatg ggtgtcgtcc gcccgctgac tgaagtcgag
     1261 atggaccatt accgcgagcc gttcctgaat cctgttgacc gcgagccact gtggcgcttc
     1321 ccaaacgagc tgccaatcgc cggtgagcca gcgaacatcg tcgcgctggt cgaagaatac
     1381 atggactggc tgcaccagtc ccctgtcccg aagctgctgt tctggggcac cccaggcgtt
     1441 ctgatcccac cggccgaagc cgctcgcctg gccaaaagcc tgcctaactg caaggctgtg
     1501 gacatcggcc cgggtctgaa tctgctgcaa gaagacaacc cggacctgat cggcagcgag
     1561 atcgcgcgct ggctgtcgac gctcgagatt tccggcgagc caaccactaa gagcaggatc
     1621 accagcgagg gcgagtacat ccccctggac cagatcgaca tcaacgtgtt ctgctacgag
     1681 aacgaggtgc aaagtcagcc tatcctgaac acaaaggaaa tggctccaca gtctaagcct
     1741 cccgaagagc ttgagatgtc cagtatgcca agtcccgtgg ctcccctccc tgccaggact
     1801 gaaggagtga ttgacatgag gagtatgtca tctattgata gcttcatctc ttgcgcaaca
     1861 gatttccccg aggctactcg attctaaact cgagcctcta gaactatagt gagtcgtatt
     1921 acgtagatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     1981 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     2041 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     2101 gggaggtgtg ggaggttttt taattcgcgg ccgcggatcc agatcgatct gcgaagatac
     2161 ggccacgggt gctcttgatc ctgtggctga ttttggactg tgctgctcgc agctgctgat
     2221 gaatcacata cttcctccat tttcttccac tgattgactg ttataatttc cctaatttcc
     2281 aggtcaaggt gctgtgcatt gtggtaatag atgtgacatg acgtcacttc caaaggacca
     2341 atgaacatgt ctgaccaatt tcatataatg tgaaaacgat tttcataggc agaataaata
     2401 acatttaaat taaactgggc atcagcgcaa ttcaattggt ttggtaatag caagggaaaa
     2461 tagaatgaag tgatctccaa aaaataagta ctttttgact gtaaataaaa ttgtaaggag
     2521 taaaaagtac ttttttttct aaaaaaatgt aattaagtaa aagtaaaagt attgattttt
     2581 aattgtactc aagtaaagta aaaatcccca aaaataatac ttaagtacag taatcaagta
     2641 aaattactca agtactttac acctctggtt cttgaccccc taccttcagc aagcccagca
     2701 gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag
     2761 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     2821 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     2881 gcctaatgag tgagccaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     2941 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3001 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3061 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3121 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3181 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3241 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3301 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3361 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3421 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3481 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3541 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3601 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3661 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3721 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3781 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3841 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3901 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3961 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4021 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4081 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4141 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4201 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     4261 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     4321 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     4381 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     4441 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     4501 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4561 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4621 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4681 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4741 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4801 tgttgaatac tcatactctt ccggtaccgg catatggttc ttgacagagg tgtaaaaagt
     4861 actcaaaaat tttactcaag tgaaagtaca agtacttagg gaaaatttta ctcaattaaa
     4921 agtaaaagta tctggctaga atcttacttg agtaaaagta aaaaagtact ccattaaaat
     4981 tgtacttgag tattaaggaa gtaaaagtaa aagcaagaaa gaaaactaga gattcttgtt
     5041 taagctttta atctcaaaaa acattaaatg aaatgcatac aaggttttat cctgctttag
     5101 aactgtttgt atttaattat caaactataa gacagacaat ctaatgccag tacacgctac
     5161 tcaaagttgt aaaacctcag atttaacttc agtagaagct gattctcaaa attgttagtg
     5221 tcaagcctag ctcttttggg gctgaaaagc aatcctgcag tgctgaaaag cctctcacag
     5281 gcagccgatg cgggaagagg tgtattagtc ttgatagaga ggctgcaaat agcaggaaac
     5341 gtgagcagag actccctggt gtctgaaaca caggccagat gggccctcga cggtatcgat
     5401 aagaaaacta gagattcttg tttaagcttg aggctgctgt cagggagctc atggacttcg
     5461 agaattaacc ctcactaaag ggaatacgaa gttatccgga gtactgtcct ccgacactag
     5521 aggtcggagt actgtcctcc gacgcaaggc ggagtactgt cctccgacgc aaggtcggag
     5581 tactgtcctc cgacactaga ggtcggagta ctgtcctccg ccatacttat ccggagtact
     5641 gtcctccgac actagaggtc ggagtactgt cctccgacgc aaggcggagt actgtcctcc
     5701 gacgcaaggt cggagtactg tcctccgaca ctagaggtcg gagtactgtc ctccgccata
     5761 cgtagcaggg tagactgtag agggtatata atggaaccca tcgcgtctca gcc
//
