LOCUS       sequence-230345-        9539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UAS-driven expresion of Voltron in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9539)
  AUTHORS   Abdelfattah AS, Kawashima T, Singh A, Novak O, Liu H, Shuai Y, Huang
            YC, Campagnola L, Seeman SC, Yu J, Zheng J, Grimm JB, Patel R, 
            Friedrich J, Mensh BD, Paninski L, Macklin JJ, Murphy GJ, Podgorski 
            K, Lin BJ, Chen TW, Turner GC, Liu Z, Koyama M, Svoboda K, Ahrens 
            MB, Lavis LD, Schreiter ER
  TITLE     Bright and photostable chemigenetic indicators for extended in vivo 
            voltage imaging.
  JOURNAL   Science. 2019 Aug 1. pii: science.aav6416. doi: 
            10.1126/science.aav6416.
  PUBMED    31371562
REFERENCE   2  (bases 1 to 9539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2019 Aug 1. pii: science.aav6416. doi: 10.1126/science.aav6416."
COMMENT     Sequence Label: p10xUAS-IVS- Syn21-Voltron-p10
FEATURES             Location/Qualifiers
     source          1..9539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..243
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     190..211
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          258..324
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     primer_bind     complement(2729..2747)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2814..2918
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2919..2987,2988..3779)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3137..3156)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3950..4538
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4439..4458
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    4625..4694
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(4919..4937)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5389..5460,5862..6135,6210..6864,6926..7241,7445..7576,
                     7647..8261)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    9291..9385
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    9419..9513
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 agcgccggag tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc
       61 aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta
      121 aacaatctgc agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt
      181 aaatcaactg caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact
      241 ctgaatagat ctaaaaggta ggttcaacca ctgatgccta ggcacaccga aacgactaac
      301 cctaattctt atcctttact tcaggcggcc gcggctcgag aacttaaaaa aaaaaatcaa
      361 aatggctgac gtggaaaccg agaccggcat gattgcacag tggattgtct ttgctattat
      421 ggctgctgct gctattgctt ttggagtggc tgtgcacttt cggccttcag agctgaagag
      481 cgcatactat atcaacattg ccatctgcac tatcgccgct accgcttact atgcaatggc
      541 cgtgaactac caggacctga caatgaatgg tgaaaggcag gtggtctacg caagatatat
      601 taactgggtg ctgaccacac cactgctcct gctcgatctc atcgtcatga ccaagatggg
      661 cggagtgatg atttcttggg tcatcggcgc agacattttc atgatcgtgt ttggtattct
      721 gggcgccttc gaggatgaac acaagttcaa atgggtgtac tttatcgctg gatgtgtgat
      781 gcaggcagtc ctgacatacg ggatgtataa cgccacttgg aaagacgatc tgaagaaaag
      841 ccccgagtac catagctcct atgtcagtct gctcgtcttc ctgtcaatcc tctgggtgtt
      901 ttatcctgtc gtgtgggctt tcgggtctgg tagtggcgtg ctgtccgtcg acaatgaggc
      961 cattctcatg ggaatcctgg atgtgctcgc taagccactg tttggaatgg ggtgcctcat
     1021 tgcccatgag actatcttca agatcggtac tggctttcca ttcgaccccc attatgtgga
     1081 agtcctgggc gagcgcatgc actacgtcga tgttggtccg cgcgatggca cccctgtgct
     1141 gttcctgcac ggtaacccga cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc
     1201 accgacccat cgctgcattg ctccagacct gatcggtatg ggcaaatccg acaaaccaga
     1261 cctgggttat ttcttcgacg accacgtccg cttcatggat gccttcatcg aagccctggg
     1321 tctggaagag gtcgtcctgg tcattcacga ctggggctcc gctctgggtt tccactgggc
     1381 caagcgcaat ccagagcgcg tcaaaggtat tgcatttatg gagttcatcc gccctatccc
     1441 gacctgggac gaatggccag aatttgcccg cgagaccttc caggccttcc gcaccaccga
     1501 cgtcggccgc aagctgatca tcgatcagaa cgtttttatc gagggtacgc tgccgatggg
     1561 tgtcgtccgc ccgctgactg aagtcgagat ggaccattac cgcgagccgt tcctgaatcc
     1621 tgttgaccgc gagccactgt ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc
     1681 gaacatcgtc gcgctggtcg aagaatacat ggactggctg caccagtccc ctgtcccgaa
     1741 gctgctgttc tggggcaccc caggcgttct gatcccaccg gccgaagccg ctcgcctggc
     1801 caaaagcctg cctaactgca aggctgtgga catcggcccg ggtctgaatc tgctgcaaga
     1861 agacaacccg gacctgatcg gcagcgagat cgcgcgctgg ctgtcgacgc tcgagatttc
     1921 cggcgagcca accactaaga gcaggatcac cagcgagggc gagtacatcc ccctggacca
     1981 gatcgacatc aacgtgttct gctacgagaa cgaggtgtaa tctagaatga atcgttttta
     2041 aaataacaaa tcaattgttt tataatattc gtacgattct ttgattatgt aataaaatgt
     2101 gatcattagg aagattacga aaaatataaa aaatatgagt tctgtgtgta taacaaatgc
     2161 tgtaaacgcc acaattgtgt ttgttgcaaa taaacccatg attatttgat taaaattgtt
     2221 gttttctttg ttcatagaca atagtgtgtt ttgcctaaac gtgtactgca taaactccat
     2281 gcgagtgtat agcgagctag tggctaacgc ttgccccacc aaagtagatt cgtcaaaatc
     2341 ctcaatttca tcaccctcct ccaagtttaa catttggccg tcggaattaa cttctaaaga
     2401 tgccacataa tctaataaat gaaatagaga ttcaaacgtg gcgtcatcgt ccgtttcgac
     2461 catttccgaa aagaactcgg gcataaactc tatgatttct ctggacgtgg tgttgtcgaa
     2521 actctcaaag tacgcagtca ggaacgtgcg cgacatgtcg tcgggaaact cgcgcggaaa
     2581 catgttgttg taaccgaacg ggtcccatag cgccaaaacc aaatctgcca gcgtcaatag
     2641 aatgagcacg atgccgacaa tggagctggc ttggatagcg attcgagtta acggccggcc
     2701 gtttaaacga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     2761 gtcatgataa taatggtttc ttagactcag gtggcacttt tcggggaaat gtgcgcggaa
     2821 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     2881 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     2941 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     3001 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     3061 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     3121 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     3181 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     3241 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     3301 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     3361 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     3421 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     3481 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     3541 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     3601 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     3661 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     3721 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     3781 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     3841 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     3901 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3961 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     4021 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     4081 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     4141 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     4201 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4261 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4321 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4381 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4441 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4501 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4561 tacggttcct ggccttttgc tggccttttg ctcacatgtt accgtcgacg atgtaggtca
     4621 cggtctcgaa gccgcggtgc gggtgccagg gcgtgccctt gggctccccg ggcgcgtact
     4681 ccacctcacc catctggtcc atcatgatga acgggtcgag gtggcggtag ttgatcccgg
     4741 cgaacgcgcg gcgcaccggg aagccctcgc cctcgaaacc gctgggcgcg gtggtcacgg
     4801 tgagcacggg acgtgcgacg gcgtcggcgg gtgcggatac gcggggcagc gtcagcgggt
     4861 tctcgacggt cacggcgggc atgtcgacaa gccgaacata tgggcgcgcc tagtatgtat
     4921 gtaagttaat aaaacccatt tttgcggaaa gtagataaaa aaaacatttt ttttttttac
     4981 tgcactggat atcattgaac ttatctgatc agttttaaat ttacttcgat ccaagggtat
     5041 ttgatgtacc aggttctttc gattacctct cactcaaaat gacattccac tcaaagtcag
     5101 cgctgtttgc ctccttctct gtccacagaa atatcgccgt ctctttcgcc gctgcgtccg
     5161 ctatctcttt cgccaccgtt tgtagcgtta cgtagcgtca atgtccgcct tcagttgcat
     5221 tttgtcagcg gtttcgtgac gaagctccaa gcggtttacg ccatcaatta aacacaaagt
     5281 gctgtgccaa aactcctctc gcttcttatt tttgtttgtt ttttgagtga ttggggtggt
     5341 gattggtttt gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat gggccaagag
     5401 gatcaggagc tattaattcg cggaggcagc aaacacccat ctgccgagca tctgaacaat
     5461 gtgagtagta catgtgcata catcttaagt tcacttgatc tataggaact gcgattgcaa
     5521 catcaaattg tctgcggcgt gagaactgcg acccacaaaa atcccaaacc gcaattgcac
     5581 aaacaaatag tgacacgaaa cagattattc tggtagctgt tctcgctata taagacaatt
     5641 tttgagatca tatcatgatc aagacatcta aaggcattca ttttcgacta tattcttttt
     5701 tacaaaaaat ataacaacca gatattttaa gctgatccta gatgcacaaa aaataaataa
     5761 aagtataaac ctacttcgta ggatacttcg gggtactttt tgttcggggt tagatgagca
     5821 taacgcttgt agttgatatt tgagatcccc tatcattgca gggtgacagc ggagcggctt
     5881 cgcagagctg cattaaccag ggcttcgggc aggccaaaaa ctacggcacg ctccggccac
     5941 ccagtccgcc ggaggactcc ggttcaggga gcggccaact agccgagaac ctcacctatg
     6001 cctggcacaa tatggacatc tttggggcgg tcaatcagcc gggctccgga tggcggcagc
     6061 tggtcaaccg gacacgcgga ctattctgca acgagcgaca cataccggcg cccaggaaac
     6121 atttgctcaa gaacggtgag tttctattcg cagtcggctg atctgtgtga aatcttaata
     6181 aagggtccaa ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg gcgaactttt
     6241 ggccgtgatg ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc ttgcctttcg
     6301 atcgccgcag ggcatccaag tatcgccatc cgggatgcga ctgctcaatg gccaacctgt
     6361 ggacgccaag gagatgcagg ccaggtgcgc ctatgtccag caggatgacc tctttatcgg
     6421 ctccctaacg gccagggaac acctgatttt ccaagccatg gtgcggatgc cacgacatct
     6481 gacctatcgg cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt cgctcagcaa
     6541 atgtcagcac acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg gcggagaaag
     6601 gaagcgtctg gcattcgcct ccgaggctct aaccgatccg ccgcttctga tctgcgatga
     6661 gcccacctcc ggactggact cctttaccgc ccacagcgtc gtccaggtgc tgaagaagct
     6721 gtcgcagaag ggcaagaccg tcatcctgac cattcatcag ccgtcttccg agctgtttga
     6781 gctctttgac aagatccttc tgatggccga gggcagggta gctttcttgg gcactcccag
     6841 cgaagccgtc gacttctttt cctagtgagt tcgatgtgtt tattaagggt atctagtatt
     6901 acataacatc tcaactccta tccagcgtgg gtgcccagtg tcctaccaac tacaatccgg
     6961 cggactttta cgtacaggtg ttggccgttg tgcccggacg ggagatcgag tcccgtgatc
     7021 ggatcgccaa gatatgcgac aattttgcca ttagcaaagt agcccgggat atggagcagt
     7081 tgttggccac caaaaatctg gagaagccac tggagcagcc ggagaatggg tacacctaca
     7141 aggccacctg gttcatgcag ttccgggcgg tcctgtggcg atcctggctg tcggtgctca
     7201 aggaaccact cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt tccagtggaa
     7261 acaaatgata taacgcttac aattcttgga aacaaattcg ctagatttta gatagaattg
     7321 cctgattcca cacccttctt agtttttttc aatgagatgt atagtttata gttttgcaga
     7381 agataaataa atttcattta actcgcgaat attaatgaga tgcgagtaac attttaattt
     7441 gcagatggtt gccatcttga ttggcctcat ctttttgggc caacaactca cgcaagtggg
     7501 tgtgatgaat atcaacggag ccatcttcct cttcctgacc aacatgacct ttcaaaacgt
     7561 ctttgccacg ataaatgtaa gtcatgttta gaatacattt gcatttcaat aatttactaa
     7621 ctttctaatg aatcgattcg atttaggtgt tcacctcaga gctgccagtt tttatgaggg
     7681 aggcccgaag tcgactttat cgctgtgaca catactttct gggcaaaacg attgccgaat
     7741 tgccgctttt tctcacagtg ccactggtct tcacggcgat tgcctatccg atgatcggac
     7801 tgcgggccgg agtgctgcac ttcttcaact gcctggcgct ggtcactctg gtggccaatg
     7861 tgtcaacgtc cttcggatat ctaatatcct gcgccagctc ctcgacctcg atggcgctgt
     7921 ctgtgggtcc gccggttatc ataccattcc tgctctttgg cggcttcttc ttgaactcgg
     7981 gctcggtgcc agtatacctc aaatggttgt cgtacctctc atggttccgt tacgccaacg
     8041 agggtctgct gattaaccaa tgggcggacg tggagccggg cgaaattagc tgcacatcgt
     8101 cgaacaccac gtgccccagt tcgggcaagg tcatcctgga gacgcttaac ttctccgccg
     8161 ccgatctgcc gctggactac gtgggtctgg ccattctcat cgtgagcttc cgggtgctcg
     8221 catatctggc tctaagactt cgggcccgac gcaaggagta gccgacatat atccgaaata
     8281 actgcttgtt tttttttttt accattatta ccatcgtgtt tactgtttat tgccccctca
     8341 aaaagctaat gtaattatat ttgtgccaat aaaaacaaga tatgacctat agaatacaag
     8401 tatttcccct tcgaacatcc ccacaagtag actttggatt tgtcttctaa ccaaaagact
     8461 tacacacctg cataccttac atcaaaaact cgtttatcgc tacataaaac accgggatat
     8521 attttttata tacatacttt tcaaatcgcg cgccctcttc ataattcacc tccaccacac
     8581 cacgtttcgt agttgctctt tcgctgtctc ccacccgctc tccgcaacac attcaccttt
     8641 tgttcgacga ccttggagcg actgtcgtta gttccgcgcg attcggttcg ctcaaatggt
     8701 tccgagtggt tcatttcgtc tcaatagaaa ttagtaataa atatttgtat gtacaattta
     8761 tttgctccaa tatatttgta tatatttccc tcacagctat atttattcta atttaatatt
     8821 atgacttttt aaggtaattt tttgtgacct gttcggagtg attagcgtta caatttgaac
     8881 tgaaagtgac atccagtgtt tgttccttgt gtagatgcat ctcaaaaaaa tggtgggcat
     8941 aatagtgttg tttatatata tcaaaaataa caactataat aataagaata catttaattt
     9001 agaaaatgct tggatttcac tggaactagg gcgcgcctcc ggaacataat ggtgcagggc
     9061 gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt
     9121 gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat
     9181 tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc
     9241 acgatcatgc gcacccgtgg ccagggccgc aagcttgcat gcctgcaggt cggagtactg
     9301 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
     9361 tccgagcgga gtactgtcct ccgagcggag actctagccc tagggcatgc ctgcaggtcg
     9421 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
     9481 tactgtcctc cgagcggagt actgtcctcc gagcggagac tctagcgcta gcgacgtcg
//
