LOCUS       sequence-230347-       14212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pan neuronal expression of Voltron in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14212)
  AUTHORS   Abdelfattah AS, Kawashima T, Singh A, Novak O, Liu H, Shuai Y, Huang
            YC, Campagnola L, Seeman SC, Yu J, Zheng J, Grimm JB, Patel R, 
            Friedrich J, Mensh BD, Paninski L, Macklin JJ, Murphy GJ, Podgorski 
            K, Lin BJ, Chen TW, Turner GC, Liu Z, Koyama M, Svoboda K, Ahrens 
            MB, Lavis LD, Schreiter ER
  TITLE     Bright and photostable chemigenetic indicators for extended in vivo 
            voltage imaging.
  JOURNAL   Science. 2019 Aug 1. pii: science.aav6416. doi: 
            10.1126/science.aav6416.
  PUBMED    31371562
REFERENCE   2  (bases 1 to 14212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2019 Aug 1. pii: science.aav6416. doi: 10.1126/science.aav6416."
COMMENT     Sequence Label: pTol2-elavl3-Voltron
FEATURES             Location/Qualifiers
     source          1..14212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    8191..8325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8215..8234)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8269..8288
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(8764..8951,8952..8963)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8997..9013)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8997..9013)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9050..9070)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9050..9068)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9089..9105)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9089..9105)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9102..9124)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9113..9129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9137..9143,9144..9161,9162..9167))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9182..9203
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9320..9337)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9491..10079)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9571..9590)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10250..11041,11042..11110))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10873..10892
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11111..11215)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11378..11399)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(11748..11759,11760..11922)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 gtaatataga aacggttatg tcgaggttgt tttgtccaga gatgaccttc acacagttac
       61 agccgctctg catccacaca aatggaggac ttaatcgtgg actgcattct tagaaatgat
      121 ctacaaagac aaataatgtg aaatcaagaa aggacaaaat ttaagtaagg ggatgaggga
      181 gagagagaac gaggggcaag gagaaagcat ggctcctgtc tttttctgca cccatctgtt
      241 cggagtgcag gtggagctct attcactcag ctctgcatgt gtgtttgggg ggggcaggaa
      301 gaaagggagg gcaaaaggaa gagtggagag atggtggggg ctggagggat ggggggttct
      361 cggtgatctc tcctgaaggg gataatggga gagcagcgct ttgcaatggc tgccatgtag
      421 taccctccct gcacaattag ccaatcagca gcaagctctg ccagccagaa ggacacataa
      481 aagaagaaca ttgcagcaga ggcacagaag gagcctgcga ggagctggga aatacacaca
      541 caacagcaga accacaacac cctcccctgg acacacccta ctggggatca ctgcttttct
      601 ttttttctga accatcgccc acgccacacg gagagaaatc tctctctcat catcatcctg
      661 aagaaaaccc ccttatcctc attttcacac tgctgaggaa aacctacaat cgcacgggct
      721 gagatttcct ggcgaagact gtcctttttc ctttttcttt ttttttcttt tcctttggaa
      781 actgacattt gcatttctcc attccaagcc acggcgtaat aatatctgca atccagcctg
      841 aagacctgca aatcgaagga cctagatcac tatcatcttt gtacgtcaag gatcctggtt
      901 actgtacgta taacctttct ttcttttgct ctgaccaata tgaaacacta aaatcgattc
      961 gagcagcctc tcagcatcaa ttacagtgcg tgaaaaacat tcaaatagag gcagcaaata
     1021 ttacatgtga aaatacaggc tggctaaatc caagctaatt tagaaatgtg gtcaaaacgc
     1081 atactggcac gtctaatcgc ggattcagta aacaagatta acgattagcc cagtgtataa
     1141 gtcatatgat accggcatgc gcgagagcat cgctacaccc gagctggctt cattttcgga
     1201 ggaaaatcaa aacattgctt tctcctgccg tgcgaaccat tcgtcataaa ccgtaatacg
     1261 caacatacat ttattactac atccgttaat tagcgataat tagccgttat taacaaagag
     1321 cgctgaggaa ttcctctcaa atagcggagg tgcggcggag gcagaggggc gtaaaagggc
     1381 acatgcgtgc ctggctcaaa aaaggatgct ccagactgaa actcagagac aaaacacgat
     1441 gcctcggagg ctgagagccc atcgagagga aaggcaaaga aaagggtcga gtcactcaga
     1501 gggggagggg agatattgtg catccattgg tttgaattga agcaggcaga ataaaaatcc
     1561 ttgcgattat gcttcgtgag agatcgcgag agaggaaaag gctaaatgcc acgtatgcaa
     1621 atggataaat gtcatctatt tcttcgcgag tagacatttt gtggacaacg agagtgtaaa
     1681 taagcgatat gaggggaata caaaggcctt gagggaaagc gttttgggcg tgtcttgtcg
     1741 aaaagaagac agcgatggca cgcgctcaga accaccatct tcacgtagac aatccgcgtg
     1801 aaaacaataa caaaactgct cattttaaca cgagcaggtg tttgaaacca cccaaaaaag
     1861 attgtctaaa atattaagat taattaatga cgtttaaatg tccaggcctg atataaatga
     1921 agcgtgtagt aagagtaatc gcctttgttt cttttcagag atacgttttc agagattcag
     1981 ggaattatat cttcaataaa ataatgtttg atttcattat tttaaagctt tttaagaagt
     2041 aaaaatacac attattcatt tacattttat tttatttaaa ggcctatggt cttgaaaata
     2101 ataagtggaa tatataggct atatattcgt ctaaggacag ccggtgttat tttatagtga
     2161 cctcagacaa aagccgagac aaaacgaggc gcttgccttt cattttaatc cttaatagac
     2221 ggtgtgatga atgaatgaat gaatgaataa atgaatgaat gacgggccct tcggcatgtt
     2281 gttccgcagt cctccgtgag cacgagtctc taccgcagtg ccaagcacat aaaaccgttg
     2341 agtcagataa ccttctccag gcgcgtatac acaattcaag gacaagcaca tcacaactat
     2401 acacaacaca acacaaccgc ctaaacgcac gagctcatca atcgcaattt aaaggcatgt
     2461 cagtcaaaga aaaaggcggc gttatttaaa accaatgttc aacaatattt gtatgctgac
     2521 tagcccgggc gcgcacgcta taagatcaca aggccttgtt ttttcccttt attcaaaata
     2581 tcattaatta ttacaggtca taaaacaaaa caatgcgaat tacaatgatt taaagactat
     2641 tgatttaaca ataatcagtt aattatagaa taatattata atattttaca tacaaatgaa
     2701 agatgacatt atttgatgac attgtaatta aaatatatgc aatatatttt tacgttttta
     2761 ttaattaggc tattactatt attataatta tcattatttt tattattatt attattatta
     2821 ttaaatagtc ttgcgaaata agagtgaagg cagttgcaat gttcattgtt cccggagagt
     2881 cccgcagcct ctggcctcaa cacaaagcgc tactgtttac attaatattc atagtgctgc
     2941 catggtctct gtagaggagg aacgggtggc tcttttgttc gctagtgtca atacgtttat
     3001 tcagcaaaaa gcagctcgga tttgtgaatg cccaacaagc acacgcgcac tccaatcaaa
     3061 ggacgagagg ataacgattt taaacgattc gcaaacgtgt ttatttgtaa agacgacatc
     3121 aagagcttaa tgtccacttg aaaagaaata aatatgcccg cggcgccaag gatattttga
     3181 atgtagtcgt tcatggtgag ccactcccga attcagcact ctggagagcg accaaagagg
     3241 aatagcggaa tagcaactac ttgagccatt tctctccgtt tgatggcgtt catgattaaa
     3301 aatataatca cgtgattttt tatacatata taaatatata cataaagtaa cgctttcctt
     3361 ttgagaatac ataaatattt attcgcgtga taaatcaatg atcttatatt tatttgcacg
     3421 accgaaatta gccaaattca ccaattaaaa aaaaaataag ccaacaaaaa agaagggttt
     3481 atccgttcaa ttttgacttg tgtcctgttg ttttacagct gccactgtga tcccttaagc
     3541 tgcagtgaga gtgtggctaa tgccttttgt ttaagataat cctgtaatct gttaccgaaa
     3601 cggcctattg acaagccggc attcacattt cagtcaggag gcccgtccag acatgtacat
     3661 ttatgaatta cgaatgataa aattaagatc tgcattaggc gtcagaaatg tcacgaacac
     3721 ccattcattt cccacagcca ataatggatc atgctgggaa gcgctatcct cgcagtctca
     3781 aaattaatgt tgacatgttg cgtagagcta cagattagat cagccatatg ctttatgtgt
     3841 ttacctcagc ggatcttcag caagatgtgt ttattttaag aaaatggctt cctcgctgcc
     3901 tgcacagggg cattaaggaa gtgacgtgag cgtcctcaca gtcaaattac tttatctcat
     3961 gctgctttca ggcctctgct tattttatta ttattatttt aaattagggg gaatcacggt
     4021 ggcgttgtgg gtagggtagc gcgatcacct tacagcaaga aggtcgctgg ttcgagctct
     4081 ggctgggtca gttggcattt ctgtgtggag tttaaattga attgaaattg aataaactaa
     4141 attggcccta gtgtatgtgt gtgaatgtaa gtgtgcatgg gtgtttccca gtgttgggtt
     4201 tgactggaag agcatccgct tcgtaaaaca tatgctggat aagttggcgg ttcatttcac
     4261 tgtggtgacc gctgattaat aaagggacta agctgaaaat gaaatgaatt aatacatttt
     4321 aaatgagctt ttgctgacat gtttatgatt acaaatagtg aaattgtatg ttaatttacg
     4381 ataagtgcat tgttctaaat tcatttttat gtagcagctg gtttttcttc catttgtgtc
     4441 attcaacaga acatttttct aaaaaagtga acttaaattt tctcctccct ctgacaaggc
     4501 ttgatggtaa tttcagccga ctctaattac agactcacta aaaggaatcg gtgcagtcat
     4561 ttatctttat cagcgcgctg gtgggaaggt aataggtttt gcttatgagt tgtttgcgtg
     4621 cgagtctggg tccttgtgta ggcgatgctg atgcactgca ttaaatattg agggagaagt
     4681 cgctcaaaaa tagcaaagta caggctgggg caggaacgtc aaagcccgct cagtggccac
     4741 gtttcttgtt tcgtaagtcc tatttttaat agcgataatt caggctcagt agaaggaact
     4801 ccagcactat aagcagtctc tcgcctgtct cggcgagtta tgtattttgg gatgaagtgc
     4861 tcttccacct ctacctctgc tgtgctggcg ccagtatctg ggctgtaaga tagtgtacgt
     4921 gtcagtctgt gtggccgaag agcctgtgtg ttgtgtaact cactacattc gtcgctgtgg
     4981 atggctgact tgccatgggg aggtttactt cagttcacga atcacattcg ccacgatctt
     5041 gtcactttgt gtcaagactc gctctctgtg tgtcaacagc tctgctgttt gaggttgaaa
     5101 ttctgaactt gagatatatc ccaggatata ttagggctac tgccaacgga aaaagcaaat
     5161 ggtgccatgg ccatttgatt gatcaaacaa acaccctttt gcttgggtga cagtgaaatg
     5221 tcagatccat gtttgtactg ctttaataac ttgcctctct gagctttctc cttgtcagtg
     5281 tcaatttgcc tgagatataa attccggttg ccccgaaagc ttattttatt aaaaaaaagc
     5341 taacatgcac tcatacaacg tgcgcacata catgtgaggc ttgctaaaag tcaagaagca
     5401 agccaatagc aagagtcgtc agactgctgt tacctgggca acctggttag aggtaaggct
     5461 caaggcaaga ataaatcatt ccactgaaga gacacagcgt tacagcgaga tgagagcaag
     5521 aggcgatagt gaagggagaa caaaagaaca gcctacccac actagtgcag atctacctgt
     5581 ttgagctact gattgttcag tttgattaga tatcagacaa ttctgctatc tccaattgca
     5641 ttgctgcatg aattcctaga aatcatttta atcaccaaaa actagtaggg acacttgtgg
     5701 cgtatatgat tctgcctgtt gattgtgggg atccaacaga aagatcatga atgcattgtt
     5761 aattaagtcg gtgagacacc ctgttccacc ctaggggccg ttctggggaa agagtgcttt
     5821 cagtcagcta aagatgacta atatgtgaac atatattact acacatgcca atttgtacat
     5881 tctggatagt taccagccct gggaaaacat cgaaaaaaat aaatcaacat agataaaatt
     5941 ggcaaatccc ttctggtacg atgcagagtg cttgctctga gaacttgtgc attgtgacag
     6001 aaagcgaccg ttaaaaactg aaagtagcat ctacaacctg catacatctg ttctttgaaa
     6061 atcccctgta ggccaaatta agcatcacgc tgtgctccac agcaacacgg atgaaccgaa
     6121 aacccagaca caaacgcaca cacttttcac ataaacaagg tttagttatg ctagctatgc
     6181 tttttcgtcc tccctttgtc atcagcggcg agtgacgtaa cacagtttga ccctggacag
     6241 cgtaaagggg gggttgggaa tgagtgaaag gactgtgaca tctgcagggc caggggctga
     6301 tggagagagc tgaagtgagg gaggtcagag gaggaggaag ggtgggatat tgtcctcctc
     6361 aggccctatc cctctcagac gcattgattt ttaacctctc agcgagaatg ccaagcatta
     6421 cacccctcaa attatggttc ctcacccaac ctgttatatt ttccacctgc agataattac
     6481 cggtgccatc atggctgacg tggaaaccga gaccggcatg attgcacagt ggattgtctt
     6541 tgctattatg gctgctgctg ctattgcttt tggagtggct gtgcactttc ggccttcaga
     6601 gctgaagagc gcatactata tcaacattgc catctgcact atcgccgcta ccgcttacta
     6661 tgcaatggcc gtgaactacc aggacctgac aatgaatggt gaaaggcagg tggtctacgc
     6721 aagatatatt aactgggtgc tgaccacacc actgctcctg ctcgatctca tcgtcatgac
     6781 caagatgggc ggagtgatga tttcttgggt catcggcgca gacattttca tgatcgtgtt
     6841 tggtattctg ggcgccttcg aggatgaaca caagttcaaa tgggtgtact ttatcgctgg
     6901 atgtgtgatg caggcagtcc tgacatacgg gatgtataac gccacttgga aagacgatct
     6961 gaagaaaagc cccgagtacc atagctccta tgtcagtctg ctcgtcttcc tgtcaatcct
     7021 ctgggtgttt tatcctgtcg tgtgggcttt cgggtctggt agtggcgtgc tgtccgtcga
     7081 caatgaggcc attctcatgg gaatcctgga tgtgctcgct aagccactgt ttggaatggg
     7141 gtgcctcatt gcccatgaga ctatcttcaa gatcggtact ggctttccat tcgaccccca
     7201 ttatgtggaa gtcctgggcg agcgcatgca ctacgtcgat gttggtccgc gcgatggcac
     7261 ccctgtgctg ttcctgcacg gtaacccgac ctcctcctac gtgtggcgca acatcatccc
     7321 gcatgttgca ccgacccatc gctgcattgc tccagacctg atcggtatgg gcaaatccga
     7381 caaaccagac ctgggttatt tcttcgacga ccacgtccgc ttcatggatg ccttcatcga
     7441 agccctgggt ctggaagagg tcgtcctggt cattcacgac tggggctccg ctctgggttt
     7501 ccactgggcc aagcgcaatc cagagcgcgt caaaggtatt gcatttatgg agttcatccg
     7561 ccctatcccg acctgggacg aatggccaga atttgcccgc gagaccttcc aggccttccg
     7621 caccaccgac gtcggccgca agctgatcat cgatcagaac gtttttatcg agggtacgct
     7681 gccgatgggt gtcgtccgcc cgctgactga agtcgagatg gaccattacc gcgagccgtt
     7741 cctgaatcct gttgaccgcg agccactgtg gcgcttccca aacgagctgc caatcgccgg
     7801 tgagccagcg aacatcgtcg cgctggtcga agaatacatg gactggctgc accagtcccc
     7861 tgtcccgaag ctgctgttct ggggcacccc aggcgttctg atcccaccgg ccgaagccgc
     7921 tcgcctggcc aaaagcctgc ctaactgcaa ggctgtggac atcggcccgg gtctgaatct
     7981 gctgcaagaa gacaacccgg acctgatcgg cagcgagatc gcgcgctggc tgtcgacgct
     8041 cgagatttcc ggcgagccaa ccactaagag caggatcacc agcgagggcg agtacatccc
     8101 cctggaccag atcgacatca acgtgttctg ctacgagaac gaggtgtaac cggtggatcc
     8161 tggccgtcgt tttactgtac catcgatgat gatccagaca tgataagata cattgatgag
     8221 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     8281 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     8341 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     8401 ctctacaaat gtggtatggc tgattatgat cctctagatc agatctgcga agatacggcc
     8461 acgggtgctc ttgatcctgt ggctgatttt ggactgtgct gctcgcagct gctgatgaat
     8521 cacatacttc ctccattttc ttccactgat tgactgttat aatttcccta atttccaggt
     8581 caaggtgctg tgcattgtgg taatagatgt gacatgacgt cacttccaaa ggaccaatga
     8641 acatgtctga ccaatttcat ataatgtgaa aacgattttc ataggcagaa taaataacat
     8701 ttaaattaaa ctgggcatca gcgcaattca attggtttgg taatagcaag ggaaaataga
     8761 atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt aaggagtaaa
     8821 aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg atttttaatt
     8881 gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat caagtaaaat
     8941 tactcaagta ctttacacct ctggttcttg accccctacc ttcagcaagc ccagcagatc
     9001 cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag
     9061 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     9121 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     9181 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     9241 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     9301 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     9361 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     9421 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     9481 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     9541 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     9601 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     9661 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     9721 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     9781 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     9841 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     9901 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     9961 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    10021 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    10081 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    10141 gcattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    10201 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    10261 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    10321 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    10381 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    10441 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    10501 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    10561 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    10621 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    10681 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    10741 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    10801 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    10861 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    10921 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10981 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    11041 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    11101 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    11161 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    11221 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
    11281 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
    11341 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
    11401 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
    11461 acgctggcta agaactcatc agcctccccg gtccatctac ccacgtacca atgcaccaat
    11521 tggccacaat gacggctact acatggtgcc attccttcct ctttatagga atggagacta
    11581 cctcctgtcc aacaatgctc ttggatacga gtacgcctac ctgttggacc caggtcattg
    11641 cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc agacacccat
    11701 atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag aggtgtaaaa
    11761 agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt ttactcaatt
    11821 aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt actccattaa
    11881 aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact agagattctt
    11941 gtttaagctt cccgggtgtc gacgaattct cgagctctat tcctaaagac cttgggtgac
    12001 caaaatctta ttttaataaa taaaactgtt tattaaaact tttttgtttc aaagaaccat
    12061 atgtatagtg aaatttataa aaatatcaat ttttaaaaag ctggtgtact catttatgtt
    12121 atgaactcta aaaccatata ctgactgcaa gtgatgatgt atagagtgat gtttacgagt
    12181 aaacatattt agttgtatac atcctactga gcacattttg atgtatgaaa taacattaca
    12241 agctttatcc aaattaagcc attttaaaac actgccaatt gaaaatacaa atcctggaaa
    12301 aaatcgtctt tagcgcagtc atttgagcca tcctaatccg ttacctcaga ccataataag
    12361 aagggataac actagctgta gcaatggaac acatctgttt cacacaatca tatctcctgc
    12421 gccggtgcta agcagattca gcgtgatcat aacatgcttt ccactcataa atgtaaattt
    12481 acaatttgca catgtaaaac agacactttt gagatattgg ataaaaaaac aagagtatat
    12541 tgcttagttt catccaccag tcatccccac agcgtttgga aggccataaa aagtgtctaa
    12601 aatcaatgat cattgaaaga gcacaagaga gactcttacg ctgtaatgcc actggggaca
    12661 aaagtgacag tctcttaatg ggctcttctg gaggggctcc tgaacattaa aaattatcag
    12721 cgaaattacc gaaagagctt caagcaactg gcatgcttga tcctctgcgt cggggcggtg
    12781 aataggtgct tcagatgccc tcttacccac gggctggatt cagctgcccc gctaccagcg
    12841 gagaccccct aatgagcctc tgcaattaag tttattcatg ttaagtgtga acggggtgcg
    12901 tgcggaactg tgggcagcta acagacctgg gttctttgtg ccacaagtgc tgcctttatt
    12961 cggctcacaa agcagaaaac aacacccgca cctataatgg cgccctcggc tgggtctaag
    13021 aaacgtggcg agttgacaga gcagagtggg cggggttaag acagactgac agcgggaccc
    13081 atctccatcc tcttattaac gcttaacgag tgccttcctc atgcaatatt catcgccact
    13141 aatatcatcc aagctctgag ctgagctggc cacttatgta aggcaattat gtaaaatatc
    13201 agacagggcc cacactcaga atctgactgg ggtagagacg cgggacgaga accgagagca
    13261 agaactgaaa gtgaaagtga ccactaaagg gaggagagga cagaggggca ggatgtgtca
    13321 agattaccag agaacacttg gccagaaatg cgcaaccatt ggagctctcc ggattaccca
    13381 aaggttaacg agtttgaacg cctctgccca ctcgcccatc tctgatggtt tcccaagaac
    13441 tcctcaagca aaatatatat aattgtgtgt attatgcaca gacacgagaa aatgctgttt
    13501 ttctgatctg cattacagca catttgcccg ccaacgacaa taccacccac tcggtacctc
    13561 gctgactcct gatgcctgat acctgcgcgg tgactgtcta caatctgcat aatcaagaga
    13621 agttgtgttg aagacgagcg ccacacaacc gtttccacaa ggtcacccaa ggccggtgca
    13681 gatgtaggtg aggtctccat aaacagactg aaataaacac atcctccgct gggaacaaca
    13741 accccctcac gcctcatgca tttccataag cctacatgca tctcttccaa cttatggaga
    13801 ctcgcaccta ccaacatccg cacaacaaag atatacagag cgcgctccct caggtcaagg
    13861 ccctgtgggg gtctgtgcag aaataggtca tttgtcacac atcaagtcct ggggcaggag
    13921 atgcattata gatgagacca aacagcctgt ctcggtgagc tctacccact ccctgagact
    13981 agaaatgggg gaagggagct tgagataaca accgctgcaa tcactgtgtc gatgtttaat
    14041 atcagcacca accgggaaca ataaggagat gggtgcattc atgttcacat cttaccagtc
    14101 aagtatcatc gaaccggctt gataaccaca cctcgtgtaa tagctgagca gatagttgtc
    14161 attttaaagc gttggccttt gtcgattatg taatgcgcac attcaacaca tg
//
