LOCUS       sequence-230352-        9668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LexAop2-driven expression of Voltron in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9668)
  AUTHORS   Abdelfattah AS, Kawashima T, Singh A, Novak O, Liu H, Shuai Y, Huang
            YC, Campagnola L, Seeman SC, Yu J, Zheng J, Grimm JB, Patel R, 
            Friedrich J, Mensh BD, Paninski L, Macklin JJ, Murphy GJ, Podgorski 
            K, Lin BJ, Chen TW, Turner GC, Liu Z, Koyama M, Svoboda K, Ahrens 
            MB, Lavis LD, Schreiter ER
  TITLE     Bright and photostable chemigenetic indicators for extended in vivo 
            voltage imaging.
  JOURNAL   Science. 2019 Aug 1. pii: science.aav6416. doi: 
            10.1126/science.aav6416.
  PUBMED    31371562
REFERENCE   2  (bases 1 to 9668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2019 Aug 1. pii: science.aav6416. doi: 10.1126/science.aav6416."
COMMENT     Sequence Label: p13xLexAOP2-IVS-Syn21-Voltron-p10
FEATURES             Location/Qualifiers
     source          1..9668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        123..361
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     308..329
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          376..442
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     primer_bind     complement(2847..2865)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2932..3036
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3037..3105,3106..3897)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3255..3274)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4068..4656
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4557..4576
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    4743..4812
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(5037..5055)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5507..5578,5980..6253,6328..6982,7044..7359,7563..7694,
                     7765..8379)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 taagtactgt acatccatac agtaagtact gtacatccat acagtaagta ctgtacatcc
       61 atacagtaag tactgtacat ccatacagta agcggagact ctagcactag tgacgtcgag
      121 cgccggagta taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa
      181 agtgaacacg tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa
      241 caatctgcag taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa
      301 atcaactgca actactgaaa tctgccaaga agtaattatt gaatacaaga agagaactct
      361 gaatagatct aaaaggtagg ttcaaccact gatgcctagg cacaccgaaa cgactaaccc
      421 taattcttat cctttacttc aggcggccgc ggctcgagaa cttaaaaaaa aaaatcaaaa
      481 tggctgacgt ggaaaccgag accggcatga ttgcacagtg gattgtcttt gctattatgg
      541 ctgctgctgc tattgctttt ggagtggctg tgcactttcg gccttcagag ctgaagagcg
      601 catactatat caacattgcc atctgcacta tcgccgctac cgcttactat gcaatggccg
      661 tgaactacca ggacctgaca atgaatggtg aaaggcaggt ggtctacgca agatatatta
      721 actgggtgct gaccacacca ctgctcctgc tcgatctcat cgtcatgacc aagatgggcg
      781 gagtgatgat ttcttgggtc atcggcgcag acattttcat gatcgtgttt ggtattctgg
      841 gcgccttcga ggatgaacac aagttcaaat gggtgtactt tatcgctgga tgtgtgatgc
      901 aggcagtcct gacatacggg atgtataacg ccacttggaa agacgatctg aagaaaagcc
      961 ccgagtacca tagctcctat gtcagtctgc tcgtcttcct gtcaatcctc tgggtgtttt
     1021 atcctgtcgt gtgggctttc gggtctggta gtggcgtgct gtccgtcgac aatgaggcca
     1081 ttctcatggg aatcctggat gtgctcgcta agccactgtt tggaatgggg tgcctcattg
     1141 cccatgagac tatcttcaag atcggtactg gctttccatt cgacccccat tatgtggaag
     1201 tcctgggcga gcgcatgcac tacgtcgatg ttggtccgcg cgatggcacc cctgtgctgt
     1261 tcctgcacgg taacccgacc tcctcctacg tgtggcgcaa catcatcccg catgttgcac
     1321 cgacccatcg ctgcattgct ccagacctga tcggtatggg caaatccgac aaaccagacc
     1381 tgggttattt cttcgacgac cacgtccgct tcatggatgc cttcatcgaa gccctgggtc
     1441 tggaagaggt cgtcctggtc attcacgact ggggctccgc tctgggtttc cactgggcca
     1501 agcgcaatcc agagcgcgtc aaaggtattg catttatgga gttcatccgc cctatcccga
     1561 cctgggacga atggccagaa tttgcccgcg agaccttcca ggccttccgc accaccgacg
     1621 tcggccgcaa gctgatcatc gatcagaacg tttttatcga gggtacgctg ccgatgggtg
     1681 tcgtccgccc gctgactgaa gtcgagatgg accattaccg cgagccgttc ctgaatcctg
     1741 ttgaccgcga gccactgtgg cgcttcccaa acgagctgcc aatcgccggt gagccagcga
     1801 acatcgtcgc gctggtcgaa gaatacatgg actggctgca ccagtcccct gtcccgaagc
     1861 tgctgttctg gggcacccca ggcgttctga tcccaccggc cgaagccgct cgcctggcca
     1921 aaagcctgcc taactgcaag gctgtggaca tcggcccggg tctgaatctg ctgcaagaag
     1981 acaacccgga cctgatcggc agcgagatcg cgcgctggct gtcgacgctc gagatttccg
     2041 gcgagccaac cactaagagc aggatcacca gcgagggcga gtacatcccc ctggaccaga
     2101 tcgacatcaa cgtgttctgc tacgagaacg aggtgtaatc tagaatgaat cgtttttaaa
     2161 ataacaaatc aattgtttta taatattcgt acgattcttt gattatgtaa taaaatgtga
     2221 tcattaggaa gattacgaaa aatataaaaa atatgagttc tgtgtgtata acaaatgctg
     2281 taaacgccac aattgtgttt gttgcaaata aacccatgat tatttgatta aaattgttgt
     2341 tttctttgtt catagacaat agtgtgtttt gcctaaacgt gtactgcata aactccatgc
     2401 gagtgtatag cgagctagtg gctaacgctt gccccaccaa agtagattcg tcaaaatcct
     2461 caatttcatc accctcctcc aagtttaaca tttggccgtc ggaattaact tctaaagatg
     2521 ccacataatc taataaatga aatagagatt caaacgtggc gtcatcgtcc gtttcgacca
     2581 tttccgaaaa gaactcgggc ataaactcta tgatttctct ggacgtggtg ttgtcgaaac
     2641 tctcaaagta cgcagtcagg aacgtgcgcg acatgtcgtc gggaaactcg cgcggaaaca
     2701 tgttgttgta accgaacggg tcccatagcg ccaaaaccaa atctgccagc gtcaatagaa
     2761 tgagcacgat gccgacaatg gagctggctt ggatagcgat tcgagttaac ggccggccgt
     2821 ttaaacgaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     2881 catgataata atggtttctt agactcaggt ggcacttttc ggggaaatgt gcgcggaacc
     2941 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3001 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3061 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3121 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3181 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3241 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3301 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3361 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3421 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3481 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3541 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     3601 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     3661 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     3721 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     3781 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     3841 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     3901 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     3961 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4021 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4081 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4141 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4201 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4261 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4321 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4381 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4441 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4501 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4561 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4621 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4681 cggttcctgg ccttttgctg gccttttgct cacatgttac cgtcgacgat gtaggtcacg
     4741 gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg cgcgtactcc
     4801 acctcaccca tctggtccat catgatgaac gggtcgaggt ggcggtagtt gatcccggcg
     4861 aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc tgggcgcggt ggtcacggtg
     4921 agcacgggac gtgcgacggc gtcggcgggt gcggatacgc ggggcagcgt cagcgggttc
     4981 tcgacggtca cggcgggcat gtcgacaagc cgaacatatg ggcgcgccta gtatgtatgt
     5041 aagttaataa aacccatttt tgcggaaagt agataaaaaa aacatttttt ttttttactg
     5101 cactggatat cattgaactt atctgatcag ttttaaattt acttcgatcc aagggtattt
     5161 gatgtaccag gttctttcga ttacctctca ctcaaaatga cattccactc aaagtcagcg
     5221 ctgtttgcct ccttctctgt ccacagaaat atcgccgtct ctttcgccgc tgcgtccgct
     5281 atctctttcg ccaccgtttg tagcgttacg tagcgtcaat gtccgccttc agttgcattt
     5341 tgtcagcggt ttcgtgacga agctccaagc ggtttacgcc atcaattaaa cacaaagtgc
     5401 tgtgccaaaa ctcctctcgc ttcttatttt tgtttgtttt ttgagtgatt ggggtggtga
     5461 ttggttttgg gtgggtaagc aggggaaagt gtgaaaaatc ccggcaatgg gccaagagga
     5521 tcaggagcta ttaattcgcg gaggcagcaa acacccatct gccgagcatc tgaacaatgt
     5581 gagtagtaca tgtgcataca tcttaagttc acttgatcta taggaactgc gattgcaaca
     5641 tcaaattgtc tgcggcgtga gaactgcgac ccacaaaaat cccaaaccgc aattgcacaa
     5701 acaaatagtg acacgaaaca gattattctg gtagctgttc tcgctatata agacaatttt
     5761 tgagatcata tcatgatcaa gacatctaaa ggcattcatt ttcgactata ttctttttta
     5821 caaaaaatat aacaaccaga tattttaagc tgatcctaga tgcacaaaaa ataaataaaa
     5881 gtataaacct acttcgtagg atacttcggg gtactttttg ttcggggtta gatgagcata
     5941 acgcttgtag ttgatatttg agatccccta tcattgcagg gtgacagcgg agcggcttcg
     6001 cagagctgca ttaaccaggg cttcgggcag gccaaaaact acggcacgct ccggccaccc
     6061 agtccgccgg aggactccgg ttcagggagc ggccaactag ccgagaacct cacctatgcc
     6121 tggcacaata tggacatctt tggggcggtc aatcagccgg gctccggatg gcggcagctg
     6181 gtcaaccgga cacgcggact attctgcaac gagcgacaca taccggcgcc caggaaacat
     6241 ttgctcaaga acggtgagtt tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa
     6301 gggtccaatt accaatttga aactcagttt gcggcgtggc ctatccgggc gaacttttgg
     6361 ccgtgatggg cagttccggt gccggaaaga cgaccctgct gaatgccctt gcctttcgat
     6421 cgccgcaggg catccaagta tcgccatccg ggatgcgact gctcaatggc caacctgtgg
     6481 acgccaagga gatgcaggcc aggtgcgcct atgtccagca ggatgacctc tttatcggct
     6541 ccctaacggc cagggaacac ctgattttcc aagccatggt gcggatgcca cgacatctga
     6601 cctatcggca gcgagtggcc cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat
     6661 gtcagcacac gatcatcggt gtgcccggca gggtgaaagg tctgtccggc ggagaaagga
     6721 agcgtctggc attcgcctcc gaggctctaa ccgatccgcc gcttctgatc tgcgatgagc
     6781 ccacctccgg actggactcc tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt
     6841 cgcagaaggg caagaccgtc atcctgacca ttcatcagcc gtcttccgag ctgtttgagc
     6901 tctttgacaa gatccttctg atggccgagg gcagggtagc tttcttgggc actcccagcg
     6961 aagccgtcga cttcttttcc tagtgagttc gatgtgttta ttaagggtat ctagtattac
     7021 ataacatctc aactcctatc cagcgtgggt gcccagtgtc ctaccaacta caatccggcg
     7081 gacttttacg tacaggtgtt ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg
     7141 atcgccaaga tatgcgacaa ttttgccatt agcaaagtag cccgggatat ggagcagttg
     7201 ttggccacca aaaatctgga gaagccactg gagcagccgg agaatgggta cacctacaag
     7261 gccacctggt tcatgcagtt ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag
     7321 gaaccactcc tcgtaaaagt gcgacttatt cagacaacgg tgagtggttc cagtggaaac
     7381 aaatgatata acgcttacaa ttcttggaaa caaattcgct agattttaga tagaattgcc
     7441 tgattccaca cccttcttag tttttttcaa tgagatgtat agtttatagt tttgcagaag
     7501 ataaataaat ttcatttaac tcgcgaatat taatgagatg cgagtaacat tttaatttgc
     7561 agatggttgc catcttgatt ggcctcatct ttttgggcca acaactcacg caagtgggtg
     7621 tgatgaatat caacggagcc atcttcctct tcctgaccaa catgaccttt caaaacgtct
     7681 ttgccacgat aaatgtaagt catgtttaga atacatttgc atttcaataa tttactaact
     7741 ttctaatgaa tcgattcgat ttaggtgttc acctcagagc tgccagtttt tatgagggag
     7801 gcccgaagtc gactttatcg ctgtgacaca tactttctgg gcaaaacgat tgccgaattg
     7861 ccgctttttc tcacagtgcc actggtcttc acggcgattg cctatccgat gatcggactg
     7921 cgggccggag tgctgcactt cttcaactgc ctggcgctgg tcactctggt ggccaatgtg
     7981 tcaacgtcct tcggatatct aatatcctgc gccagctcct cgacctcgat ggcgctgtct
     8041 gtgggtccgc cggttatcat accattcctg ctctttggcg gcttcttctt gaactcgggc
     8101 tcggtgccag tatacctcaa atggttgtcg tacctctcat ggttccgtta cgccaacgag
     8161 ggtctgctga ttaaccaatg ggcggacgtg gagccgggcg aaattagctg cacatcgtcg
     8221 aacaccacgt gccccagttc gggcaaggtc atcctggaga cgcttaactt ctccgccgcc
     8281 gatctgccgc tggactacgt gggtctggcc attctcatcg tgagcttccg ggtgctcgca
     8341 tatctggctc taagacttcg ggcccgacgc aaggagtagc cgacatatat ccgaaataac
     8401 tgcttgtttt ttttttttac cattattacc atcgtgttta ctgtttattg ccccctcaaa
     8461 aagctaatgt aattatattt gtgccaataa aaacaagata tgacctatag aatacaagta
     8521 tttccccttc gaacatcccc acaagtagac tttggatttg tcttctaacc aaaagactta
     8581 cacacctgca taccttacat caaaaactcg tttatcgcta cataaaacac cgggatatat
     8641 tttttatata catacttttc aaatcgcgcg ccctcttcat aattcacctc caccacacca
     8701 cgtttcgtag ttgctctttc gctgtctccc acccgctctc cgcaacacat tcaccttttg
     8761 ttcgacgacc ttggagcgac tgtcgttagt tccgcgcgat tcggttcgct caaatggttc
     8821 cgagtggttc atttcgtctc aatagaaatt agtaataaat atttgtatgt acaatttatt
     8881 tgctccaata tatttgtata tatttccctc acagctatat ttattctaat ttaatattat
     8941 gactttttaa ggtaattttt tgtgacctgt tcggagtgat tagcgttaca atttgaactg
     9001 aaagtgacat ccagtgtttg ttccttgtgt agatgcatct caaaaaaatg gtgggcataa
     9061 tagtgttgtt tatatatatc aaaaataaca actataataa taagaataca tttaatttag
     9121 aaaatgcttg gatttcactg gaactagggc gcgcctccgg aacataatgg tgcagggcgc
     9181 tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
     9241 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
     9301 attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
     9361 gatcatgcgc acccgtggcc agggccgcaa gcttgcatgc ctgcaggtta ctgtacatcc
     9421 atacagtaag tactgtacat ccatacagta agtactgtac atccatacag taagtactgt
     9481 acatccatac agtaagtact gtacatccat acagtaagcg gagactctag ccctagggca
     9541 tgcctgcagg ttactgtaca tccatacagt aagtactgta catccataca gtaagtactg
     9601 tacatccata cagtaagcgg agactctagc gctagcgcat gcctgcaggt tactgtacat
     9661 ccatacag
//
