LOCUS       sequence-230354-       10108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Raj B, O'Hanlon D, Vessey JP, Pan Q, Ray D, Buckley NJ, Miller FD, 
            Blencowe BJ
  TITLE     Cross-regulation between an alternative splicing activator and a 
            transcription repressor controls neurogenesis.
  JOURNAL   Mol Cell. 2011 Sep 2;43(5):843-50.
  PUBMED    21884984
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-09-2"; volume: "43(5)"; pages: "843-50"
COMMENT     Sequence Label: pLDPuro-MmnSR100N
FEATURES             Location/Qualifiers
     source          1..10108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    48..69
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(84..89,90..107,108..114)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    122..138
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     127..149
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     146..162
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     146..162
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     181..201
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        183..201
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        229..455
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             456..636
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    683..808
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1301..1534
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1719..1763
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2061..2081
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     intron          2393..3315
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3272..3292
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3338..3362
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3411..3434
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(5259..5283)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5294..5359
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5430..5547
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5596..6106
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6025..6043
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6128..6727
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6128..6147)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6624..6644
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6855..7088
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7160..7281
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7197..7216)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7251..7270
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7321..7456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7476..7495)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7477..7495)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7505..7522)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7505..7521)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7514..7536)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7663..8118
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7750..7769)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7960..7981
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8144..8248
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8249..8317,8318..9109)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8467..8486)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9280..9868
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9769..9788
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10022..10039
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
       61 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      121 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      181 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
      241 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      301 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      361 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      421 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      481 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      541 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      601 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      661 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      721 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
      781 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
      841 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
      901 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
      961 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1021 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1081 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1141 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1201 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1261 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1321 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1381 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1441 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1501 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1561 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1621 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1681 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1741 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     1801 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     1861 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     1921 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     1981 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc
     2041 gagcggccgc ccccttcacc gagggcctat ttcccatgat tccttcatat ttgcatatac
     2101 gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag
     2161 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
     2221 gttttaaaat ggactatcat atggtgccta gagaaggtgg cgcggggtaa actgggaaag
     2281 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     2341 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
     2401 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
     2461 tacttccacc tggctccagt acgtgattct tgatcccgag ctggagccag gggcgggcct
     2521 tgcgctttag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     2581 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     2641 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     2701 aatgcgggcc aggatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     2761 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     2821 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     2881 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     2941 agatggccgc ttcccggccc tgctccaggg ggctcaaaat ggaggacgcg gcgctcggga
     3001 gagcgggcgg gtgagtcacc cacacaaagg aaaggggcct ttccgtcctc agccgtcgct
     3061 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctggagcttt
     3121 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     3181 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     3241 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     3301 tttcttccat ttcaggtgtc gtgaaggtac cacgcgtaca agtttgtaca aaaaagcagg
     3361 ctaccatgga ctacaaagac catgacggtg atacatatga tcatgacatc gattacaagg
     3421 atgacgatga caaggctagc ctgcagcagg gcgagaaaca gctgtttgag aagttctgga
     3481 aagggacctt caaagcagtg gccactccgc gtcccgagag catcatcgtc gccagtatca
     3541 cagcccgcaa gccgatgcca aggacagagc cccagagcag tctcttgctc ccagatcagg
     3601 atggaccttc agaaaagctg ggccaacatc tggcccctga ggccctgggt accaacagct
     3661 ggggaagaga gaaggcctgc agggaactgg atcctgccag agcacacagt gcctctcaag
     3721 acagagaccc gacaccacca ccttcctcca gggggaaaaa gaaaaagaag aaatcaacca
     3781 ggaaaaagag gaggaggtcc ccgtcctaca gcccatcacc agtcaagaag aagaagaaga
     3841 aaagctccaa gaaacataag cgacacaggt ctttctccaa gaaaaggaga cacagctcct
     3901 gtagccctaa aagcaaaaga cgggaagaga agaggcacaa aaaacaatct cgaagccgga
     3961 agtctcaccg acatagacat caccgatgcc cctcaaggtc ccagagttcg gaactgcgct
     4021 cccccagctg cgagagcagg catcgaggtc ggtcccctga ggaagggcgg aaatcccgcc
     4081 gaacacactc ccgccgctgc tccaagaacc actgcaaggt cagcccagac gcccggtcca
     4141 gccacctgcc cagccagccc ctcccgaggc tcggcttcct gtcagccagg ggtgtaatca
     4201 ctgggtcggg gtccgcggct gacctcttta gcaaatcggc cagccctctc gccgccaccc
     4261 gagggcgctc gcaggagtac gactcgggca atgacacgtc ctcgccgccc tccacgcaaa
     4321 ccagctcggc caggtctcgg ggccaggaga aggggagtcc cggtggggat ctaagcaaga
     4381 gtcgagacct caactgtggc aacacctccg attcagggaa ctccttcacc acctcctccc
     4441 cccagaacaa gggggctgtc ttggagactg tctctcccgc ctgcaggagc agagagtcaa
     4501 gagggtttca gtccccatgt ctccagtgtg cagaggtgaa gaagtctagt ttggtcccat
     4561 ccacagcccg gagctccccc atcaaagagt gctcccgtag ctcctcctac accagcactc
     4621 gatcctccag tccctcatcc aggtccccaa accccagagc ttcccccagg tacacccgga
     4681 gtcggtccac ctcctctgaa aaaaggtcct actctcgctc tcccagctat tcgtccaagt
     4741 ctggcaagag gagcccacct agcagaagct ccaggtctcg tcgaagtccc agctactctc
     4801 gctacagccc cagcagggaa cgggacctaa agtatggtga gaaagaacca cagccccgag
     4861 aacgtgcgcg caggaggcga cggtcctact cacccatgag gaagcgcagg agagactccc
     4921 caagccacct ggaggctcgg aggataacca gtgcccgcaa acgccccatc ccctactacc
     4981 gacccagccc ctcctcgtcc agcagcctca gcagcgcctc ctcctggtac agcagcagca
     5041 gcagcagcag cagcagtagc agccgctccc ctagccgcag ttactcccgg agccggagtc
     5101 ccagtcggag ccacagcagc cggagccaga cccggagcag gacgcggacc agccgcagct
     5161 ccagttctcg cagcctcagc ctgggctccc ggagccgcag tcggaaccgg agcctgagct
     5221 acagctcggc agaaagctat gccagcacga ggcgctaaac ccagctttct tgtacaaagt
     5281 ggttgattct agagactaca aagaccatga cggtgattat aaagatcatg acatcgatta
     5341 caaggatgac gatgacaagt aaggatcccg ggtggcatcc ctgtgttcga attctcgacc
     5401 tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     5461 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     5521 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
     5581 tggccgcggc tcgagggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca
     5641 gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg
     5701 cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc
     5761 cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg
     5821 ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca
     5881 gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg
     5941 gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt
     6001 gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc
     6061 ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg atccaccgga
     6121 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     6181 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     6241 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     6301 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     6361 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     6421 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     6481 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     6541 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     6601 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6661 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     6721 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc
     6781 atcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6841 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     6901 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6961 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7021 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7081 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7141 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     7201 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7261 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7321 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7381 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7441 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     7501 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     7561 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     7621 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     7681 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     7741 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     7801 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     7861 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     7921 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     7981 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     8041 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     8101 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     8161 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     8221 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     8281 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     8341 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     8401 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     8461 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     8521 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     8581 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     8641 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     8701 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     8761 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     8821 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     8881 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     8941 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     9001 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     9061 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     9121 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     9181 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     9241 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     9301 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     9361 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     9421 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     9481 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9541 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9601 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9661 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9721 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9781 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9841 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9901 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     9961 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    10021 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
    10081 gcgttggccg attcattaat gcagctgg
//
