LOCUS       sequence-230357-       14401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pan neuronal expression of soma-localized Voltron in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14401)
  AUTHORS   Abdelfattah AS, Kawashima T, Singh A, Novak O, Liu H, Shuai Y, Huang
            YC, Campagnola L, Seeman SC, Yu J, Zheng J, Grimm JB, Patel R, 
            Friedrich J, Mensh BD, Paninski L, Macklin JJ, Murphy GJ, Podgorski 
            K, Lin BJ, Chen TW, Turner GC, Liu Z, Koyama M, Svoboda K, Ahrens 
            MB, Lavis LD, Schreiter ER
  TITLE     Bright and photostable chemigenetic indicators for extended in vivo 
            voltage imaging.
  JOURNAL   Science. 2019 Aug 1. pii: science.aav6416. doi: 
            10.1126/science.aav6416.
  PUBMED    31371562
REFERENCE   2  (bases 1 to 14401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2019 Aug 1. pii: science.aav6416. doi: 10.1126/science.aav6416."
COMMENT     Sequence Label: pTol2-elavl3-Voltron-ST
FEATURES             Location/Qualifiers
     source          1..14401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    5224..5358
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5248..5267)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5302..5321
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(5797..5984,5985..5996)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6030..6046)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6030..6046)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6083..6103)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6083..6101)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6122..6138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6122..6138)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6135..6157)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6146..6162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6170..6176,6177..6194,6195..6200))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6215..6236
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6353..6370)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6524..7112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6604..6623)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7283..8074,8075..8143))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7906..7925
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8144..8248)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8411..8432)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(8781..8792,8793..8955)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 cccgcggcgc caaggatatt ttgaatgtag tcgttcatgg tgagccactc ccgaattcag
       61 cactctggag agcgaccaaa gaggaatagc ggaatagcaa ctacttgagc catttctctc
      121 cgtttgatgg cgttcatgat taaaaatata atcacgtgat tttttataca tatataaata
      181 tatacataaa gtaacgcttt ccttttgaga atacataaat atttattcgc gtgataaatc
      241 aatgatctta tatttatttg cacgaccgaa attagccaaa ttcaccaatt aaaaaaaaaa
      301 taagccaaca aaaaagaagg gtttatccgt tcaattttga cttgtgtcct gttgttttac
      361 agctgccact gtgatccctt aagctgcagt gagagtgtgg ctaatgcctt ttgtttaaga
      421 taatcctgta atctgttacc gaaacggcct attgacaagc cggcattcac atttcagtca
      481 ggaggcccgt ccagacatgt acatttatga attacgaatg ataaaattaa gatctgcatt
      541 aggcgtcaga aatgtcacga acacccattc atttcccaca gccaataatg gatcatgctg
      601 ggaagcgcta tcctcgcagt ctcaaaatta atgttgacat gttgcgtaga gctacagatt
      661 agatcagcca tatgctttat gtgtttacct cagcggatct tcagcaagat gtgtttattt
      721 taagaaaatg gcttcctcgc tgcctgcaca ggggcattaa ggaagtgacg tgagcgtcct
      781 cacagtcaaa ttactttatc tcatgctgct ttcaggcctc tgcttatttt attattatta
      841 ttttaaatta gggggaatca cggtggcgtt gtgggtaggg tagcgcgatc accttacagc
      901 aagaaggtcg ctggttcgag ctctggctgg gtcagttggc atttctgtgt ggagtttaaa
      961 ttgaattgaa attgaataaa ctaaattggc cctagtgtat gtgtgtgaat gtaagtgtgc
     1021 atgggtgttt cccagtgttg ggtttgactg gaagagcatc cgcttcgtaa aacatatgct
     1081 ggataagttg gcggttcatt tcactgtggt gaccgctgat taataaaggg actaagctga
     1141 aaatgaaatg aattaataca ttttaaatga gcttttgctg acatgtttat gattacaaat
     1201 agtgaaattg tatgttaatt tacgataagt gcattgttct aaattcattt ttatgtagca
     1261 gctggttttt cttccatttg tgtcattcaa cagaacattt ttctaaaaaa gtgaacttaa
     1321 attttctcct ccctctgaca aggcttgatg gtaatttcag ccgactctaa ttacagactc
     1381 actaaaagga atcggtgcag tcatttatct ttatcagcgc gctggtggga aggtaatagg
     1441 ttttgcttat gagttgtttg cgtgcgagtc tgggtccttg tgtaggcgat gctgatgcac
     1501 tgcattaaat attgagggag aagtcgctca aaaatagcaa agtacaggct ggggcaggaa
     1561 cgtcaaagcc cgctcagtgg ccacgtttct tgtttcgtaa gtcctatttt taatagcgat
     1621 aattcaggct cagtagaagg aactccagca ctataagcag tctctcgcct gtctcggcga
     1681 gttatgtatt ttgggatgaa gtgctcttcc acctctacct ctgctgtgct ggcgccagta
     1741 tctgggctgt aagatagtgt acgtgtcagt ctgtgtggcc gaagagcctg tgtgttgtgt
     1801 aactcactac attcgtcgct gtggatggct gacttgccat ggggaggttt acttcagttc
     1861 acgaatcaca ttcgccacga tcttgtcact ttgtgtcaag actcgctctc tgtgtgtcaa
     1921 cagctctgct gtttgaggtt gaaattctga acttgagata tatcccagga tatattaggg
     1981 ctactgccaa cggaaaaagc aaatggtgcc atggccattt gattgatcaa acaaacaccc
     2041 ttttgcttgg gtgacagtga aatgtcagat ccatgtttgt actgctttaa taacttgcct
     2101 ctctgagctt tctccttgtc agtgtcaatt tgcctgagat ataaattccg gttgccccga
     2161 aagcttattt tattaaaaaa aagctaacat gcactcatac aacgtgcgca catacatgtg
     2221 aggcttgcta aaagtcaaga agcaagccaa tagcaagagt cgtcagactg ctgttacctg
     2281 ggcaacctgg ttagaggtaa ggctcaaggc aagaataaat cattccactg aagagacaca
     2341 gcgttacagc gagatgagag caagaggcga tagtgaaggg agaacaaaag aacagcctac
     2401 ccacactagt gcagatctac ctgtttgagc tactgattgt tcagtttgat tagatatcag
     2461 acaattctgc tatctccaat tgcattgctg catgaattcc tagaaatcat tttaatcacc
     2521 aaaaactagt agggacactt gtggcgtata tgattctgcc tgttgattgt ggggatccaa
     2581 cagaaagatc atgaatgcat tgttaattaa gtcggtgaga caccctgttc caccctaggg
     2641 gccgttctgg ggaaagagtg ctttcagtca gctaaagatg actaatatgt gaacatatat
     2701 tactacacat gccaatttgt acattctgga tagttaccag ccctgggaaa acatcgaaaa
     2761 aaataaatca acatagataa aattggcaaa tcccttctgg tacgatgcag agtgcttgct
     2821 ctgagaactt gtgcattgtg acagaaagcg accgttaaaa actgaaagta gcatctacaa
     2881 cctgcataca tctgttcttt gaaaatcccc tgtaggccaa attaagcatc acgctgtgct
     2941 ccacagcaac acggatgaac cgaaaaccca gacacaaacg cacacacttt tcacataaac
     3001 aaggtttagt tatgctagct atgctttttc gtcctccctt tgtcatcagc ggcgagtgac
     3061 gtaacacagt ttgaccctgg acagcgtaaa gggggggttg ggaatgagtg aaaggactgt
     3121 gacatctgca gggccagggg ctgatggaga gagctgaagt gagggaggtc agaggaggag
     3181 gaagggtggg atattgtcct cctcaggccc tatccctctc agacgcattg atttttaacc
     3241 tctcagcgag aatgccaagc attacacccc tcaaattatg gttcctcacc caacctgtta
     3301 tattttccac ctgcagataa ttaccggtat ggctgacgtg gaaaccgaga ccggcatgat
     3361 tgcacagtgg attgtctttg ctattatggc tgctgctgct attgcttttg gagtggctgt
     3421 gcactttcgg ccttcagagc tgaagagcgc atactatatc aacattgcca tctgcactat
     3481 cgccgctacc gcttactatg caatggccgt gaactaccag gacctgacaa tgaatggtga
     3541 aaggcaggtg gtctacgcaa gatatattaa ctgggtgctg accacaccac tgctcctgct
     3601 cgatctcatc gtcatgacca agatgggcgg agtgatgatt tcttgggtca tcggcgcaga
     3661 cattttcatg atcgtgtttg gtattctggg cgccttcgag gatgaacaca agttcaaatg
     3721 ggtgtacttt atcgctggat gtgtgatgca ggcagtcctg acatacggga tgtataacgc
     3781 cacttggaaa gacgatctga agaaaagccc cgagtaccat agctcctatg tcagtctgct
     3841 cgtcttcctg tcaatcctct gggtgtttta tcctgtcgtg tgggctttcg ggtctggtag
     3901 tggcgtgctg tccgtcgaca atgaggccat tctcatggga atcctggatg tgctcgctaa
     3961 gccactgttt ggaatggggt gcctcattgc ccatgagact atcttcaaga tcggtactgg
     4021 ctttccattc gacccccatt atgtggaagt cctgggcgag cgcatgcact acgtcgatgt
     4081 tggtccgcgc gatggcaccc ctgtgctgtt cctgcacggt aacccgacct cctcctacgt
     4141 gtggcgcaac atcatcccgc atgttgcacc gacccatcgc tgcattgctc cagacctgat
     4201 cggtatgggc aaatccgaca aaccagacct gggttatttc ttcgacgacc acgtccgctt
     4261 catggatgcc ttcatcgaag ccctgggtct ggaagaggtc gtcctggtca ttcacgactg
     4321 gggctccgct ctgggtttcc actgggccaa gcgcaatcca gagcgcgtca aaggtattgc
     4381 atttatggag ttcatccacc ctatcccgac ctgggacgaa tggccagaat ttgcccgcga
     4441 gaccttccag gccttccgca ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt
     4501 ttttatcgag ggtacgctgc cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga
     4561 ccattaccgc gagccgttcc tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa
     4621 cgagctgcca atcgccggtg agccagcgaa catcgtcgcg ctggtcgaag aatacatgga
     4681 ctggctgcac cagtcccctg tcccgaagct gctgttctgg ggcaccccag gcgttctgat
     4741 cccaccggcc gaagccgctc gcctggccaa aagcctgcct aactgcaagg ctgtggacat
     4801 cggcccgggt ctgaatctgc tgcaagaaga caacccggac ctgatcggca gcgagatcgc
     4861 gcgctggctg tcgacgctcg agatttccgg cgagccaacc actaagagca ggatcaccag
     4921 cgagggcgag tacatccccc tggaccagat cgacatcaac gtgttctgct acgagaacga
     4981 ggtgcaaagt cagcctatcc tgaacacaaa ggaaatggct ccacagtcta agcctcccga
     5041 agagcttgag atgtccagta tgccaagtcc cgtggctccc ctccctgcca ggactgaagg
     5101 agtgattgac atgaggagta tgtcatctat tgatagcttc atctcttgcg caacagattt
     5161 ccccgaggct actcgattct aaccggtgga tcctggccgt cgttttactg taccatcgat
     5221 gatgatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     5281 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     5341 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     5401 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     5461 gatcctctag atcagatctg cgaagatacg gccacgggtg ctcttgatcc tgtggctgat
     5521 tttggactgt gctgctcgca gctgctgatg aatcacatac ttcctccatt ttcttccact
     5581 gattgactgt tataatttcc ctaatttcca ggtcaaggtg ctgtgcattg tggtaataga
     5641 tgtgacatga cgtcacttcc aaaggaccaa tgaacatgtc tgaccaattt catataatgt
     5701 gaaaacgatt ttcataggca gaataaataa catttaaatt aaactgggca tcagcgcaat
     5761 tcaattggtt tggtaatagc aagggaaaat agaatgaagt gatctccaaa aaataagtac
     5821 tttttgactg taaataaaat tgtaaggagt aaaaagtact tttttttcta aaaaaatgta
     5881 attaagtaaa agtaaaagta ttgattttta attgtactca agtaaagtaa aaatccccaa
     5941 aaataatact taagtacagt aatcaagtaa aattactcaa gtactttaca cctctggttc
     6001 ttgaccccct accttcagca agcccagcag atccactagt tctagagcgg ccgccaccgc
     6061 ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     6121 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6181 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6241 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6301 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6361 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6421 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6481 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6541 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6601 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6661 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6721 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6781 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6841 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6901 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6961 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7021 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7081 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7141 ctgacgctca gtggaacgaa aactcacgtt aaggcatttt ggtcatgaga ttatcaaaaa
     7201 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7261 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7321 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7381 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7441 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7501 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7561 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7621 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7681 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7741 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7801 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7861 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7921 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7981 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8041 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8101 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8161 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8221 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     8281 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     8341 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     8401 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     8461 agggcgaaaa accgtctatc agggcgatgg cccacgctgg ctaagaactc atcagcctcc
     8521 ccggtccatc tacccacgta ccaatgcacc aattggccac aatgacggct actacatggt
     8581 gccattcctt cctctttata ggaatggaga ctacctcctg tccaacaatg ctcttggata
     8641 cgagtacgcc tacctgttgg acccaggtca ttgcacaaca ccagaaatgc cctctgatct
     8701 gcaaaagacg tgaatatctg ttcagacacc catatccact ctgttccaca caggtcagag
     8761 gtttgtccag gagttcttga cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa
     8821 agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg gctagaatct
     8881 tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt aaggaagtaa
     8941 aagtaaaagc aagaaagaaa actagagatt cttgtttaag cttcccgggt gtcgacgaat
     9001 tctcgagctc tattcctaaa gaccttgggt gaccaaaatc ttattttaat aaataaaact
     9061 gtttattaaa acttttttgt ttcaaagaac catatgtata gtgaaattta taaaaatatc
     9121 aatttttaaa aagctggtgt actcatttat gttatgaact ctaaaaccat atactgactg
     9181 caagtgatga tgtatagagt gatgtttacg agtaaacata tttagttgta tacatcctac
     9241 tgagcacatt ttgatgtatg aaataacatt acaagcttta tccaaattaa gccattttaa
     9301 aacactgcca attgaaaata caaatcctgg aaaaaatcgt ctttagcgca gtcatttgag
     9361 ccatcctaat ccgttacctc agaccataat aagaagggat aacactagct gtagcaatgg
     9421 aacacatctg tttcacacaa tcatatctcc tgcgccggtg ctaagcagat tcagcgtgat
     9481 cataacatgc tttccactca taaatgtaaa tttacaattt gcacatgtaa aacagacact
     9541 tttgagatat tggataaaaa aacaagagta tattgcttag tttcatccac cagtcatccc
     9601 cacagcgttt ggaaggccat aaaaagtgtc taaaatcaat gatcattgaa agagcacaag
     9661 agagactctt acgctgtaat gccactgggg acaaaagtga cagtctctta atgggctctt
     9721 ctggaggggc tcctgaacat taaaaattat cagcgaaatt accgaaagag cttcaagcaa
     9781 ctggcatgct tgatcctctg cgtcggggcg gtgaataggt gcttcagatg ccctcttacc
     9841 cacgggctgg attcagctgc cccgctacca gcggagaccc cctaatgagc ctctgcaatt
     9901 aagtttattc atgttaagtg tgaacggggt gcgtgcggaa ctgtgggcag ctaacagacc
     9961 tgggttcttt gtgccacaag tgctgccttt attcggctca caaagcagaa aacaacaccc
    10021 gcacctataa tggcgccctc ggctgggtct aagaaacgtg gcgagttgac agagcagagt
    10081 gggcggggtt aagacagact gacagcggga cccatctcca tcctcttatt aacgcttaac
    10141 gagtgccttc ctcatgcaat attcatcgcc actaatatca tccaagctct gagctgagct
    10201 ggccacttat gtaaggcaat tatgtaaaat atcagacagg gcccacactc agaatctgac
    10261 tggggtagag acgcgggacg agaaccgaga gcaagaactg aaagtgaaag tgaccactaa
    10321 agggaggaga ggacagaggg gcaggatgtg tcaagattac cagagaacac ttggccagaa
    10381 atgcgcaacc attggagctc tccggattac ccaaaggtta acgagtttga acgcctctgc
    10441 ccactcgccc atctctgatg gtttcccaag aactcctcaa gcaaaatata tataattgtg
    10501 tgtattatgc acagacacga gaaaatgctg tttttctgat ctgcattaca gcacatttgc
    10561 ccgccaacga caataccacc cactcggtac ctcgctgact cctgatgcct gatacctgcg
    10621 cggtgactgt ctacaatctg cataatcaag agaagttgtg ttgaagacga gcgccacaca
    10681 accgtttcca caaggtcacc caaggccggt gcagatgtag gtgaggtctc cataaacaga
    10741 ctgaaataaa cacatcctcc gctgggaaca acaaccccct cacgcctcat gcatttccat
    10801 aagcctacat gcatctcttc caacttatgg agactcgcac ctaccaacat ccgcacaaca
    10861 aagatataca gagcgcgctc cctcaggtca aggccctgtg ggggtctgtg cagaaatagg
    10921 tcatttgtca cacatcaagt cctggggcag gagatgcatt atagatgaga ccaaacagcc
    10981 tgtctcggtg agctctaccc actccctgag actagaaatg ggggaaggga gcttgagata
    11041 acaaccgctg caatcactgt gtcgatgttt aatatcagca ccaaccggga acaataagga
    11101 gatgggtgca ttcatgttca catcttacca gtcaagtatc atcgaaccgg cttgataacc
    11161 acacctcgtg taatagctga gcagatagtt gtcattttaa agcgttggcc tttgtcgatt
    11221 atgtaatgcg cacattcaac acatggtaat atagaaacgg ttatgtcgag gttgttttgt
    11281 ccagagatga ccttcacaca gttacagccg ctctgcatcc acacaaatgg aggacttaat
    11341 cgtggactgc attcttagaa atgatctaca aagacaaata atgtgaaatc aagaaaggac
    11401 aaaatttaag taaggggatg agggagagag agaacgaggg gcaaggagaa agcatggctc
    11461 ctgtcttttt ctgcacccat ctgttcggag tgcaggtgga gctctattca ctcagctctg
    11521 catgtgtgtt tggggggggc aggaagaaag ggagggcaaa aggaagagtg gagagatggt
    11581 gggggctgga gggatggggg gttctcggtg atctctcctg aaggggataa tgggagagca
    11641 gcgctttgca atggctgcca tgtagtaccc tccctgcaca attagccaat cagcagcaag
    11701 ctctgccagc cagaaggaca cataaaagaa gaacattgca gcagaggcac agaaggagcc
    11761 tgcgaggagc tgggaaatac acacacaaca gcagaaccac aacaccctcc cctggacaca
    11821 ccctactggg gatcactgct tttctttttt tctgaaccat cgcccacgcc acacggagag
    11881 aaatctctct ctcatcatca tcctgaagaa aaccccctta tcctcatttt cacactgctg
    11941 aggaaaacct acaatcgcac gggctgagat ttcctggcga agactgtcct ttttcctttt
    12001 tctttttttt tcttttcctt tggaaactga catttgcatt tctccattcc aagccacggc
    12061 gtaataatat ctgcaatcca gcctgaagac ctgcaaatcg aaggacctag atcactatca
    12121 tctttgtacg tcaaggatcc tggttactgt acgtataacc tttctttctt ttgctctgac
    12181 caatatgaaa cactaaaatc gattcgagca gcctctcagc atcaattaca gtgcgtgaaa
    12241 aacattcaaa tagaggcagc aaatattaca tgtgaaaata caggctggct aaatccaagc
    12301 taatttagaa atgtggtcaa aacgcatact ggcacgtcta atcgcggatt cagtaaacaa
    12361 gattaacgat tagcccagtg tataagtcat atgataccgg catgcgcgag agcatcgcta
    12421 cacccgagct ggcttcattt tcggaggaaa atcaaaacat tgctttctcc tgccgtgcga
    12481 accattcgtc ataaaccgta atacgcaaca tacatttatt actacatccg ttaattagcg
    12541 ataattagcc gttattaaca aagagcgctg aggaattcct ctcaaatagc ggaggtgcgg
    12601 cggaggcaga ggggcgtaaa agggcacatg cgtgcctggc tcaaaaaagg atgctccaga
    12661 ctgaaactca gagacaaaac acgatgcctc ggaggctgag agcccatcga gaggaaaggc
    12721 aaagaaaagg gtcgagtcac tcagaggggg aggggagata ttgtgcatcc attggtttga
    12781 attgaagcag gcagaataaa aatccttgcg attatgcttc gtgagagatc gcgagagagg
    12841 aaaaggctaa atgccacgta tgcaaatgga taaatgtcat ctatttcttc gcgagtagac
    12901 attttgtgga caacgagagt gtaaataagc gatatgaggg gaatacaaag gccttgaggg
    12961 aaagcgtttt gggcgtgtct tgtcgaaaag aagacagcga tggcacgcgc tcagaaccac
    13021 catcttcacg tagacaatcc gcgtgaaaac aataacaaaa ctgctcattt taacacgagc
    13081 aggtgtttga aaccacccaa aaaagattgt ctaaaatatt aagattaatt aatgacgttt
    13141 aaatgtccag gcctgatata aatgaagcgt gtagtaagag taatcgcctt tgtttctttt
    13201 cagagatacg ttttcagaga ttcagggaat tatatcttca ataaaataat gtttgatttc
    13261 attattttaa agctttttaa gaagtaaaaa tacacattat tcatttacat tttattttat
    13321 ttaaaggcct atggtcttga aaataataag tggaatatat aggctatata ttcgtctaag
    13381 gacagccggt gttattttat agtgacctca gacaaaagcc gagacaaaac gaggcgcttg
    13441 cctttcattt taatccttaa tagacggtgt gatgaatgaa tgaatgaatg aataaatgaa
    13501 tgaatgacgg gcccttcggc atgttgttcc gcagtcctcc gtgagcacga gtctctaccg
    13561 cagtgccaag cacataaaac cgttgagtca gataaccttc tccaggcgcg tatacacaat
    13621 tcaaggacaa gcacatcaca actatacaca acacaacaca accgcctaaa cgcacgagct
    13681 catcaatcgc aatttaaagg catgtcagtc aaagaaaaag gcggcgttat ttaaaaccaa
    13741 tgttcaacaa tatttgtatg ctgactagcc cgggcgcgca cgctataaga tcacaaggcc
    13801 ttgttttttc cctttattca aaatatcatt aattattaca ggtcataaaa caaaacaatg
    13861 cgaattacaa tgatttaaag actattgatt taacaataat cagttaatta tagaataata
    13921 ttataatatt ttacatacaa atgaaagatg acattatttg atgacattgt aattaaaata
    13981 tatgcaatat atttttacgt ttttattaat taggctatta ctattattat aattatcatt
    14041 atttttatta ttattattat tattattaaa tagtcttgcg aaataagagt gaaggcagtt
    14101 gcaatgttca ttgttcccgg agagtcccgc agcctctggc ctcaacacaa agcgctactg
    14161 tttacattaa tattcatagt gctgccatgg tctctgtaga ggaggaacgg gtggctcttt
    14221 tgttcgctag tgtcaatacg tttattcagc aaaaagcagc tcggatttgt gaatgcccaa
    14281 caagcacacg cgcactccaa tcaaaggacg agaggataac gattttaaac gattcgcaaa
    14341 cgtgtttatt tgtaaagacg acatcaagag cttaatgtcc acttgaaaag aaataaatat
    14401 g
//
