LOCUS       sequence-23036-e         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Samuels Y, Wang Z, Bardelli A, Silliman N, Ptak J, Szabo S, Yan H, 
            Gazdar A, Powell SM, Riggins GJ, Willson JK, Markowitz S, Kinzler 
            KW, Vogelstein B, Velculescu VE
  TITLE     High frequency of mutations of the PIK3CA gene in human cancers.
  JOURNAL   Science. 2004 Apr 23. 304(5670):554.
  PUBMED    15016963
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2004 Apr 23. 304(5670):554."
COMMENT     Sequence Label: PIK3CA - Exon20 H1047R (pFastBac)
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             89..106
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             128..148
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttgtataaaa aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg
       61 atctcggtcc gaaaccatgt cgtactacca tcaccatcac catcacgatt acgatatccc
      121 aacgaccgaa aacctgtatt ttcagggcgc catgggatcc atgcctccaa gaccatcatc
      181 aggtgaactg tggggcatcc acttgatgcc cccaagaatc ctagtagaat gtttactacc
      241 aaatggaatg atagtgactt tagaatgcct ccgtgaggct acattaataa ccataaagca
      301 tgaactattt aaagaagcaa gaaaataccc cctccatcaa cttcttcaag atgaatcttc
      361 ttacattttc gtaagtgtta ctcaagaagc agaaagggaa gaattttttg atgaaacaag
      421 acgactttgt gaccttcggc tttttcaacc ctttttaaaa gtaattgaac cagtaggcaa
      481 ccgtgaagaa aagatcctca atcgagaaat tggttttgct atcggcatgc cagtgtgtga
      541 atttgatatg gttaaagatc cagaagtaca ggacttccga agaaatattc tgaacgtttg
      601 taaagaagct gtggatctta gggacctcaa ttcacctcat agtagagcaa tgtatgtcta
      661 tcctcccaat gtagaatctt caccagaatt gccaaagcac atatataata aa
//
