LOCUS       sequence-23038-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Samuels Y, Wang Z, Bardelli A, Silliman N, Ptak J, Szabo S, Yan H, 
            Gazdar A, Powell SM, Riggins GJ, Willson JK, Markowitz S, Kinzler 
            KW, Vogelstein B, Velculescu VE
  TITLE     High frequency of mutations of the PIK3CA gene in human cancers.
  JOURNAL   Science. 2004 Apr 23. 304(5670):554.
  PUBMED    15016963
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2004 Apr 23. 304(5670):554."
COMMENT     Sequence Label: PIK3CA - Exon9 E545K
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        51..69
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             113..136
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcgactggt tagtgaccgt cagatccgct agcgattacg ccaagctcga aattaaccct
       61 cactaaaggg aacaaaagct ggagctccac cgcggtggcg gccgccacca tggattacaa
      121 ggatgacgac gataaggccc gggcggatcc cccgggctgc aggaattcga tagttcgaac
      181 catgcctcca agaccatcat caggtgaact gtggggcatc cacttgatgc ccccaagaat
      241 cctagtagaa tgtttactac caaatggaat gatagtgact ttagaatgcc tccgcgaggc
      301 tacattaata accataaagc atgaactatt taaagaagca agaaaatacc ccctccatca
      361 acttcttcaa gatgaatctt cttacatttt cgtaagtgtt actcaagaag cagaaaggga
      421 agaatttttt gatgaaacaa gacgactttg tgaccttcgg ctttttcaac cctttttaaa
      481 agtaattgaa ccagtaggca accgtgaaga aaagatcctc gatcgagaaa ttggttttgc
      541 tatcggcatg ccagtgtgtg aatttgatat ggttaaagat ccagaagtac aggacttccg
      601 aagaaatatt ctgaacgttt gtaaagaagc tgtggatctt agggacctca attcacctca
      661 tagtagagca atgtatgtct atcctccaaa tgtagaatct tcaccagaat cgccaaagca
      721 catatacaat aaattagata aagggcaaat aatagtggtg atctgggtaa tagtttctcc
      781 aaaataatga caagcagaag tatactctga aaatcaacca tgactgtgta ccagaacaag
      841 taattgctga agcaatcagg aaaaaactcg aagtatgttg ctatcctctg aacactaaaa
      901 ctctgtg
//
