LOCUS       sequence-230390-       10874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fast anion channelrhodopsin mutant RapACR_T111C for time-resolved 
            inhibition of neuronal spiking.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10874)
  AUTHORS   Govorunova EG, Sineshchekov OA, Hemmati R, Janz R, Morelle O, 
            Melkonian M, Wong GK, Spudich JL
  TITLE     Extending the Time Domain of Neuronal Silencing with Cryptophyte 
            Anion Channelrhodopsins.
  JOURNAL   eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-0174-18. doi: 
            10.1523/ENEURO.0174-18.2018. eCollection 2018 May-Jun.
  PUBMED    30027111
REFERENCE   2  (bases 1 to 10874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eNeuro."; 
            date: "2018-07-10"; volume: "5(3). pii: eN-MNT-0174-18. doi"; pages:
            " 10.1523/ENEURO.0174-18.2018. eCollection 2018 May-Jun"
COMMENT     Sequence Label: pFUGW-RapACR_T111C-EYFP
FEATURES             Location/Qualifiers
     source          1..10874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2412..3623
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3460..3479
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(4588..4590,4591..5304)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4630..4651)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4891..4910)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5238..5259
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5329..5917
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5382..5402)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5800..5811)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5920..5936)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5921..5937)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6442..6622
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6648..6665)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6654..6878
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6924..7352
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7011..7030)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7221..7242
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7361..7381)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7366..7695
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7546..7681
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7608..7627
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7743..7790
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7809..8183
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8313..8434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8350..8369)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8404..8423
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8483..8499)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8483..8499)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8496..8518)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8507..8523
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8531..8537,8538..8555,8556..8561))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8576..8597
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8714..8731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8885..9473)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8965..8984)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9644..10435,10436..10504))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10267..10286
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10505..10609)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10684..10703)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa tcgcatgcta gctagttcga accttaatta acccgtgtcg
     2401 gctccagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
     2461 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac
     2521 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
     2581 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
     2641 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
     2701 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
     2761 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg
     2821 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
     2881 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
     2941 tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag
     3001 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt
     3061 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat
     3121 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg
     3181 ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc
     3241 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc
     3301 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg
     3361 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt
     3421 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta
     3481 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat
     3541 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc
     3601 cgtttttggc ttttttgtta gacgaagctt gggctgcagg tcgactctag aggatccatg
     3661 gactacacca gcctggacga ttttccactg gagacactgt acttccggaa cggcacagag
     3721 gtggacccct atgatcacag atgcaagctg ggcatccctg gaggctccca gggcatccac
     3781 acatttcagc tggtgtgggc actgctgtgc gcagcacagg ccctgtttct gttctccaag
     3841 tggccaaaag tgggctggga ggccatgtac atgccctgct gtgaggccct gtcttatggc
     3901 ctgctgagct ccggctacgg ctatatgaag ctgcacggcg agttccacta cctgccttgg
     3961 atcaggtacg ccgcctggct ggtgacctgc ccagtgctgc tgaaccaggt gcagggcatg
     4021 atcgacatca agatcggctc tctgaggctg gcaccaatcc agagcctggc aaacctgatc
     4081 atgatcatca tgggcgtgag cgcccacctg caggagaatc acgcctggaa gtggctgttc
     4141 tttttcgtgg gctgcgtgat gtgcgccgtg atctactata tcgcctggct gcagtttgcc
     4201 aaagtgaatg agctgtacgc cgccaccaag acagagatcg gcgataaggt gtgctttcag
     4261 ctgaagctga tcatgttttg tttctatgtg ggctggtcca tgttctctgt gatctggctg
     4321 ctgtctagcg agggcctgtg cctgatcgag gagcacatca gcgccgccgc ccacatccca
     4381 ggcgacatcc tgagcaagaa ctcctttggc ctgctgatgt ggtataccag gtggatcaca
     4441 ctgggaggag catgggatat caataagttc ctggagaagc aggccaagga gaaggcagag
     4501 gccgtgctga cccctgccga gaagaggaag gccatcatga agcaggccga ggcctgcttc
     4561 tccatggccg atgcctgtgc ggccgccgtg agcaagggcg aggagctgtt caccggggtg
     4621 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     4681 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     4741 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc
     4801 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     4861 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     4921 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     4981 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     5041 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     5101 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     5161 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
     5221 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     5281 ggcatggacg agctgtacaa gtaagaattc gatatcaagc ttatcgataa tcaacctctg
     5341 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5401 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5461 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5521 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     5581 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     5641 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     5701 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     5761 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     5821 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     5881 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac
     5941 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     6001 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     6061 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6121 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     6181 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
     6241 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
     6301 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     6361 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
     6421 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6481 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6541 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6601 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     6661 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     6721 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     6781 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     6841 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     6901 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6961 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7021 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7081 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     7141 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7201 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7261 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     7321 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     7381 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7441 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7501 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     7561 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7621 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7681 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     7741 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     7801 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     7861 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     7921 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     7981 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     8041 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     8101 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     8161 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     8221 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     8281 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     8341 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8401 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     8461 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     8521 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     8581 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     8641 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     8701 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8761 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8821 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8881 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8941 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     9001 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     9061 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     9121 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     9181 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     9241 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     9301 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     9361 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     9421 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     9481 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     9541 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9601 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9661 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9721 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9781 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9841 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9901 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9961 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    10021 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    10081 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    10141 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    10201 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    10261 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    10321 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    10381 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    10441 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    10501 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10561 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10621 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
    10681 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
    10741 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
    10801 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
    10861 cagatatacg cgtt
//
