LOCUS       sequence-230391-       10838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Step-function mutant of Guillardia theta anion channelrhodopsin 1 
            (GtACR1) with dramatically prolonged open state.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10838)
  AUTHORS   Govorunova EG, Sineshchekov OA, Hemmati R, Janz R, Morelle O, 
            Melkonian M, Wong GK, Spudich JL
  TITLE     Extending the Time Domain of Neuronal Silencing with Cryptophyte 
            Anion Channelrhodopsins.
  JOURNAL   eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-0174-18. doi: 
            10.1523/ENEURO.0174-18.2018. eCollection 2018 May-Jun.
  PUBMED    30027111
REFERENCE   2  (bases 1 to 10838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eNeuro."; 
            date: "2018-07-10"; volume: "5(3). pii: eN-MNT-0174-18. doi"; pages:
            " 10.1523/ENEURO.0174-18.2018. eCollection 2018 May-Jun"
COMMENT     Sequence Label: pFUGW-GtACR1_C102A-EYFP
FEATURES             Location/Qualifiers
     source          1..10838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2412..3623
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3460..3479
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(4552..4554,4555..5268)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4594..4615)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4855..4874)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5202..5223
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5293..5881
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5346..5366)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5764..5775)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5884..5900)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5885..5901)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6406..6586
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6612..6629)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6618..6842
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6888..7316
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6975..6994)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7185..7206
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7325..7345)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7330..7659
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7510..7645
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7572..7591
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7707..7754
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7773..8147
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8277..8398
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8314..8333)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8368..8387
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8447..8463)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8447..8463)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8460..8482)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8471..8487
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8495..8501,8502..8519,8520..8525))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8540..8561
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8678..8695)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8849..9437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8929..8948)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9608..10399,10400..10468))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10231..10250
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10469..10573)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10648..10667)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa tcgcatgcta gctagttcga accttaatta acccgtgtcg
     2401 gctccagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
     2461 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac
     2521 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
     2581 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
     2641 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
     2701 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
     2761 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg
     2821 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
     2881 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
     2941 tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag
     3001 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt
     3061 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat
     3121 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg
     3181 ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc
     3241 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc
     3301 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg
     3361 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt
     3421 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta
     3481 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat
     3541 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc
     3601 cgtttttggc ttttttgtta gacgaagctt gggctgcagg tcgactctag aggatccatg
     3661 agcagcatca cctgtgatcc cgccatctac ggcgaatgga gcagggagaa ccagttctgc
     3721 gtggagaaga gcctgatcac cctggacggc atcaagtacg tccagctggt gatggccgtc
     3781 gtgagcgcct gtcaggtgtt cttcatggtg accagagccc ccaaggtgcc ctgggaagcc
     3841 atctacctgc ccaccaccga gatgatcacc tattccctgg ccttcaccgg aaacggctac
     3901 atcagagtgg ccaatggcaa gtacctgccc tgggccagaa tggccagctg gctgtgcacc
     3961 gcccctatca tgctgggcct ggtgagcaat atggccctcg tgaagtacaa gagcatcccc
     4021 ctcaacccta tgatgatcgc cgcctccagc atctgcaccg tgttcggcat caccgcctcc
     4081 gtggtgctag acccgctgca cgtgtggctg tactgtttca tcagcagcat cttcttcatc
     4141 ttcgagatgg tggtggcctt cgccattttc gccattacca tccacgattt ccagaccatc
     4201 ggctccccca tgtccctgaa ggtggtggag aggctgaagc tgatgaggat cgtgttctac
     4261 gtgagctgga tggcctaccc tatcctgtgg agcttctcca gcaccggcgc ctgcatcatg
     4321 agcgagaaca ccagcagcgt gctgtacctg ctgggcgacg ctctgtgcaa gaacacctac
     4381 ggcatcctgc tgtgggctac aacctggggc ctgctgaacg gcaagtggga cagggattac
     4441 gtgaagggca ggaacgtgga cggcaccctg atgcctgagt acgagcagga cctggagaag
     4501 ggcaacaccg agaggtacga ggacgccaga gccggcgaga ccgcggccgc cgtgagcaag
     4561 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     4621 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     4681 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     4741 ttcggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     4801 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     4861 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     4921 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     4981 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     5041 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     5101 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac
     5161 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     5221 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaga attcgatatc
     5281 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     5341 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     5401 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     5461 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     5521 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     5581 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     5641 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     5701 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     5761 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     5821 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     5881 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
     5941 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     6001 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     6061 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     6121 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     6181 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     6241 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     6301 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     6361 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     6421 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6481 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6541 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     6601 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     6661 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     6721 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     6781 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     6841 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     6901 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6961 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     7021 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     7081 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     7141 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     7201 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     7261 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     7321 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     7381 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     7441 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     7501 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7561 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     7621 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     7681 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     7741 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     7801 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     7861 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     7921 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     7981 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     8041 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     8101 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     8161 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     8221 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     8281 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8341 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8401 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     8461 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8521 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8581 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8641 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8701 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8761 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8821 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8881 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8941 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9001 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9061 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9121 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9181 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9241 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     9301 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9361 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9421 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9481 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9541 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9601 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9661 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9721 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9781 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9841 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9901 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9961 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10021 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10081 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10141 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10201 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    10261 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10321 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10381 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10441 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10501 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10561 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
    10621 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
    10681 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
    10741 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
    10801 tgcgctgctt cgcgatgtac gggccagata tacgcgtt
//
