LOCUS       sequence-230397-        9093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 9093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pAC-GAL4
FEATURES             Location/Qualifiers
     source          1..9093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1867..4512
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     complement(5763..5780)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5763..5779)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5772..5794)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5988..6007)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6107..6129
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6167..6185)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6253..6357
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6358..6426,6427..7218)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6576..6595)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7389..7977
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7878..7897
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8131..8148
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8265..8286
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8301..8306,8307..8324,8325..8331)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8339..8355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8344..8366
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8363..8379
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8363..8379
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        join(8433..9093,1..1853)
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
ORIGIN
        1 gatgatattt taagatgatg ccagaccaaa aggcttgaat ttctgcgtct tttgccgaac
       61 gcagtgcatg tgcaattgtt gttttttgga atattcaatt ttcggactgt ccgctttgat
      121 ttcagtttct tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc
      181 aaatgcggca aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg
      241 cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg
      301 accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt
      361 tatgctgcgc tctctctagc gccatctcgc ttacgcatgc tcaacgcacc gcatgttgcc
      421 gtttcctttt atgcgtcatt ttggctcgaa ataggcaatt atttaaacaa agattagtca
      481 acgaaaacgc taaaataaat aagtctacaa tatggttact tattgccatg tgtgtgcagc
      541 caacgatagc aacaaaagca acaacacagg tggctttccc tctttcactt tttgtttgca
      601 agccgcgtgc gagcaagacg gcacgaccgg caaacgcaat tacgctgaca aagagcagac
      661 gaagttttgg cgaaaaacat caaggcgcct gatacgaatg catttgcaat aacaattgcg
      721 atatttaata ttgtttatga agctgtttga cttcaaaaca cacaaaaaaa aaaataaaac
      781 aaattatttg aaagagaatt aggaatcgga cgcttatcgt tagggtaaca acaagaaatg
      841 cttactgagt cacagcctct ggaaaactgc cgcaagccag agagagagag aaaaagaggg
      901 agagcagctt agaccgcatg tgcttgtgtg tgaggcgtct ctctcttcgt ctctgttgcg
      961 caaacgcata gactgcactg aaaaaatcga ttacctattt tttatgaatg aatatttgca
     1021 ctattactat tcaaaactat taagatagca atcacattca atagccaaat actataccac
     1081 ctgagcgatg caacgaaatg atcaatttga gcaaaaatgc tgcatattta ggacggcatc
     1141 attatagaaa tgcttcttgc tgtgtacttt tctctcgtct ggcagctgtt tcgccgttat
     1201 tgttaaaacc ggcttaagtt aggtgtgttt tctacgacta gctagtgaat gccctactag
     1261 aagatgtgtg ttgcacaaaa tgtccctgga ataaccaatt tgaagtgcag atagcagtaa
     1321 acgtaagcta atatgaatat tatttaactg taatgtttta atatcgctgg acattactaa
     1381 taaacccact ataaacacat gtacatatgt atgttttggc atacaatgag tagttgggga
     1441 aaaaatgtgt aaaagcaccg tgaccatcac agcataaaga taaccagctg aagtatcgaa
     1501 tatgagtaac ccccaaattg aatcacatgc cgcaactgat aggacccatg gaagtacact
     1561 cttcatggcg atatacaaga cacacacaag cacgaacacc cagttgcgga ggaaattctc
     1621 cgtaaatgaa aacccaatcg gcgaacaatt catacccata tatggtaaaa gttttgaacg
     1681 cgacttgaga gcggagagca ttgcggctga taaggtttta gcgctaagcg ggctttataa
     1741 aacgggctgc gggaccagtt ttcatatcac taccgtttga gttcttgtgc tgtgtggata
     1801 ctcctcccga cacaaagccg ctccatcagc cagcagtcgt ctaatccaga gaccccggat
     1861 cccaacatga agctactgtc ttctatcgaa caagcatgcg atatttgccg acttaaaaag
     1921 ctcaagtgct ccaaagaaaa accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt
     1981 cgctactctc ccaaaaccaa aaggtctccg ctgactaggg cacatctgac agaagtggaa
     2041 tcaaggctag aaagactgga acagctattt ctactgattt ttcctcgaga agaccttgac
     2101 atgattttga aaatggattc tttacaggat ataaaagcat tgttaacagg attatttgta
     2161 caagataatg tgaataaaga tgccgtcaca gatagattgg cttcagtgga gactgatatg
     2221 cctctaacat tgagacagca tagaataagt gcgacatcat catcggaaga gagtagtaac
     2281 aaaggtcaaa gacagttgac tgtatcgatt gactcggcag ctcatcatga taactccaca
     2341 attccgttgg attttatgcc cagggatgct cttcatggat ttgattggtc tgaagaggat
     2401 gacatgtcgg atggcttgcc cttcctgaaa acggacccca acaataatgg gttctttggc
     2461 gacggttctc tcttatgtat tcttcgatct attggcttta aaccggaaaa ttacacgaac
     2521 tctaacgtta acaggctccc gaccatgatt acggatagat acacgttggc ttctagatcc
     2581 acaacatccc gtttacttca aagttatctc aataattttc acccctactg ccctatcgtg
     2641 cactcaccga cgctaatgat gttgtataat aaccagattg aaatcgcgtc gaaggatcaa
     2701 tggcaaatcc tttttaactg catattagcc attggagcct ggtgtataga gggggaatct
     2761 actgatatag atgtttttta ctatcaaaat gctaaatctc atttgacgag caaggtcttc
     2821 gagtcaggtt ccataatttt ggtgacagcc ctacatcttc tgtcgcgata tacacagtgg
     2881 aggcagaaaa caaatactag ctataatttt cacagctttt ccataagaat ggccatatca
     2941 ttgggcttga atagggacct cccctcgtcc ttcagtgata gcagcattct ggaacaaaga
     3001 cgccgaattt ggtggtctgt ctactcttgg gagatccaat tgtccctgct ttatggtcga
     3061 tccatccagc tttctcagaa tacaatctcc ttcccttctt ctgtcgacga tgtgcagcgt
     3121 accacaacag gtcccaccat atatcatggc atcattgaaa cagcaaggct cttacaagtt
     3181 ttcacaaaaa tctatgaact agacaaaaca gtaactgcag aaaaaagtcc tatatgtgca
     3241 aaaaaatgct tgatgatttg taatgagatt gaggaggttt cgagacaggc accaaagttt
     3301 ttacaaatgg atatttccac caccgctcta accaatttgt tgaaggaaca cccttggcta
     3361 tcctttacaa gattcgaact gaagtggaaa cagttgtctc ttatcattta tgtattaaga
     3421 gattttttca ctaattttac ccagaaaaag tcacaactag aacaggatca aaatgatcat
     3481 caaagttatg aagttaaacg atgctccatc atgttaagcg atgcagcaca aagaactgtt
     3541 atgtctgtaa gtagctatat ggacaatcat aatgtcaccc catattttgc ctggaattgt
     3601 tcttattact tgttcaatgc agtcctagta cccataaaga ctctactctc aaactcaaaa
     3661 tcgaatgctg agaataacga gaccgcacaa ttattacaac aaattaacac tgttctgatg
     3721 ctattaaaaa aactggccac ttttaaaatc cagacttgtg aaaaatacat tcaagtactg
     3781 gaagaggtat gtgcgccgtt tctgttatca cagtgtgcaa tcccattacc gcatatcagt
     3841 tataacaata gtaatggtag cgccattaaa aatattgtcg gttctgcaac tatcgcccaa
     3901 taccctactc ttccggagga aaatgtcaac aatatcagtg ttaaatatgt ttctcctggc
     3961 tcagtagggc cttcacctgt gccattgaaa tcaggagcaa gtttcagtga tctagtcaag
     4021 ctgttatcta accgtccacc ctctcgtaac tctccagtga caataccaag aagcacacct
     4081 tcgcatcgct cagtcacgcc ttttctaggg caacagcaac agctgcaatc attagtgcca
     4141 ctgaccccgt ctgctttgtt tggtggcgcc aattttaatc aaagtgggaa tattgctgat
     4201 agctcattgt ccttcacttt cactaacagt agcaacggtc cgaacctcat aacaactcaa
     4261 acaaattctc aagcgctttc acaaccaatt gcctcctcta acgttcatga taacttcatg
     4321 aataatgaaa tcacggctag taaaattgat gatggtaata attcaaaacc actgtcacct
     4381 ggttggacgg accaaactgc gtataacgcg tttggaatca ctacagggat gtttaatacc
     4441 actacaatgg atgatgtata taactatcta ttcgatgatg aagatacccc accaaaccca
     4501 aaaaaagagt aagcggccgc gttaaccgat ccgtcgacca tgaagatcaa gatcattgcc
     4561 ccgccagagc gcaagtactc tgtctggatc ggtggctcca tcctggcttc gctgtccacc
     4621 ttccagcaga tgtggatctc caagcaggag tacgacgagt ccggcccctc cattgtgcac
     4681 cgcaagtgct tctaagaagg atcgcttgtc tgggcaagag gatcaggatc gggatggtct
     4741 tgattctgct ggaggaggag gaggagaagt cgaggaagca gcagcgaaag tgcaagtgcg
     4801 agtggtggaa gtttggagtg cagcacaaca aaatcaacaa caacaccaac tacaagatga
     4861 aaagagcgga accacctgca caccatcatc actatcatca tcgttttggg cgcatgttgt
     4921 gtggttccag cgtattaata taattaattt attccacatg agatatgata tgatatacta
     4981 tgtatttttt gttttttttt tatttgtaaa cctttaatat aacaagaact acaaaaaatg
     5041 aaaatgagcg aaaatgcata ttctgccatt ccacacacac accaacaaca cccaacacac
     5101 gcacacccac aagcttacac acacacattc gcggcatgac aaggacatca agataaagaa
     5161 gaacttaaag aagatatttc ccaaagcgca aaaagaacac acacacattg caaaacacaa
     5221 acaacacact agcgttttgt acaattcgtc agcaacctta tgtattattt tttaattatg
     5281 atgtaattat aaacaaagtg aaacaaaaat atgaaaacaa aaaggaaaat caaatctgtc
     5341 ttctctttct cccgctctcc tcgctctctg ctgctaacct cgccctctcc tctctcatct
     5401 ttttgtctgt ctctcttcca catttttgcc ggccggcaaa ataataaccc acacacactc
     5461 acacttggct gcagtttcgc gtgcgatatt cacacacatt caagcataca agcatacata
     5521 catatgtatt ttttttttat ttgtacactt ttctaattgc atgcgtatcg attgataagt
     5581 ttacgcctga aaatgttaat taaaatgtga aaatgcaact gaaaaactga tgaaatgaaa
     5641 caacaacaag cgaacaattt gctacatgtg tattgtctaa caaccgttac tgcaacggtt
     5701 gcttcggaaa agggtgaaga ggaagagggc acgaggtcga cctgcaggca tgcaagcttg
     5761 gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5821 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5881 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     5941 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     6001 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     6061 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     6121 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     6181 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     6241 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6301 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     6361 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     6421 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     6481 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     6541 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     6601 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     6661 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     6721 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     6781 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     6841 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6901 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6961 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7021 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     7081 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     7141 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     7201 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     7261 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7321 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7381 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7441 ccgctaccag cggtggtttg tttgtcggat caagagctac caactctttt tccgaaggta
     7501 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     7561 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7621 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7681 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7741 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7801 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7861 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7921 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7981 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8041 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8101 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     8161 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     8221 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     8281 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     8341 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa ttctatattc
     8401 taaaaacaca aatgatactt ctaaaaaaaa atcatgaatg gcatcaactc tgaatcaaat
     8461 ctttgcagat gcacctactt ctcatttcca ctgtcacatc atttttccag atctcgctgc
     8521 ctgttatgtg gcccacaaac caagacacgt tttatggcca ttaaagctgg ctgatcgtcg
     8581 ccaaacacca aatacataat gaatatgtac acattcgaga aagaagcgat caaagaagcg
     8641 tcttcgggcg gagtaggaga atgcggagga gaaggagaac gagctgatct agtatctctc
     8701 cacaatccaa tgccaactga ccaactggcc atattcggag caatttgaag ccaatttcca
     8761 tcgcctggcg atcgctccat tcttggctat atgtttttca ccgttacccg gggccatttt
     8821 caaagactcg tcggcaagat aagattgtgt cactcgctgt ctctcttcat ttgtcgaaga
     8881 atgctgagga atttcgcgat gacgtcggcg agtattttga agaatgagaa taatttgtat
     8941 ttatacgaaa atcagttagt ggaattttct acaaaaacat gttatctata gataattttg
     9001 ttgcaaaata tgttgactat gacaaagatt gtatgtatat acctttaatg tattctcatt
     9061 ttcttatgta tttataatgg caatgatgat act
//
