LOCUS       sequence-230400-        9108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression plasmid for Pm4CL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Pluskal T, Torrens-Spence MP, Fallon TR, De Abreu A, Shi CH, Weng JK
  TITLE     The biosynthetic origin of psychoactive kavalactones in kava.
  JOURNAL   Nat Plants. 2019 Jul 22. pii: 10.1038/s41477-019-0474-0. doi: 
            10.1038/s41477-019-0474-0.
  PUBMED    31332312
REFERENCE   2  (bases 1 to 9108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2019 Jul 22. pii: 10.1038/s41477-019-0474-0. doi: 
            10.1038/s41477-019-0474-0."
COMMENT     Sequence Label: pJKW1413
FEATURES             Location/Qualifiers
     source          1..9108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      69..1411
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    401..448
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1438..1542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1543..1611,1612..2403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1761..1780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2574..3162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3063..3082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3316..3333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3450..3471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3486..3491,3492..3509,3510..3516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3524..3540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3529..3551
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3548..3564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3548..3564
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3583..3603
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3585..3603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3621..4019
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     complement(5717..5733)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5718..5734)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      5736..5983
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5752..5770)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(6001..6020)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6002..6020)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6030..6047)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6030..6046)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6039..6061)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6187..6642
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6274..6293)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6484..6505
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6943..7350
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             7351..8445
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(8939..8958)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9058..9080
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
      121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
      181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
      241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
      301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
      361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
      421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
      481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
      541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
      601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
      661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
      721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
      781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
      841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
      901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
      961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
     1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3481 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     3601 agggaacaaa agctggagct catagcttca aaatgtttct actccttttt tactcttcca
     3661 gattttctcg gactccgcgc atcgccgtac cacttcaaaa cacccaagca cagcatacta
     3721 aatttcccct ctttcttcct ctagggtgtc gttaattacc cgtactaaag gtttggaaaa
     3781 gaaaaaagag accgcctcgt ttctttttct tcgtcgaaaa aggcaataaa aatttttatc
     3841 acgtttcttt ttcttgaaaa tttttttttt gatttttttc tctttcgatg acctcccatt
     3901 gatatttaag ttaataaacg gtcttcaatt tctcaagttt cagtttcatt tttcttgttc
     3961 tattacaact ttttttactt cttgctcatt agaaagaaag catagcaatc taatctaagt
     4021 tttctagaac tagtatgaag atggtagtag acactattgc tactgatcga tgtgtatacc
     4081 ggtctaagct gccggacatt gagatcaaga acgacatgtc gttgcacaat tattgtttcc
     4141 agaacattgg tgcttaccgg gacaatcctt gtctcatcaa tggcagcacc ggcgaggtgt
     4201 acacgtacgg cgaggtggag acgacggcga ggagggtggc cgccgggctg caccggatgg
     4261 gggtgcagca gcgggaggtg atcatgatcc tcctccccaa ctcgccggag ttcgtcttcg
     4321 ccttcctcgg cgcctccttc cgcggggcca tgtccaccac cgccaacccc ttctacacgc
     4381 cgcaggagat cgccaagcag gtcaaggcct ccggcgcgaa gctcatcgtc accatgtccg
     4441 cctacgtcga caaggtcagg gacctggccg aggagcgcgg cgtcaaagtg gtgtgcgtcg
     4501 acgcgccgcc cccggggtgc tcccacttct ccgagctgtc cggcgccgac gagtcggagc
     4561 tgcccgaggt ggatattgac cccgacgacg tggtggcgct gccatactcc tccggcacca
     4621 ccggcctccc taaaggagtg atgctcacac accgcagcca ggtgacgagc gttgcccagc
     4681 aagtcgacgg cgagaacccg aatctatact tccggccaga cgacgtcctg ctctgcgttc
     4741 ttcccctctt ccacatctac tccctcaact cggtgctctt ctgcggcctg cgcgtcgggg
     4801 cggcgatcct catcatgcag aagttcgaga tcacggcgct gatggagctg gtgcagaagt
     4861 acaaggtgac cattgcgccc atcgttccgc ccatcgttct tgccatcgcc aagagcccgc
     4921 tcgtcgacaa gtacgacttg tcgtccattc ggacggtgat gtccggcgcc gccccgatgg
     4981 ggaaggagct cgaagacgcc gtccgggcca agcttcccaa cgccaagctc ggccagggct
     5041 atgggatgac ggaggcaggg ccagtgctgt ccatgtgttt ggccttcgcc aaggagccct
     5101 tcgagatcaa gtctggttct tgcggcaccg tggtcaggaa cgcccagctc aagatcgtcg
     5161 acccagaaac cggtgcctac ctgcccagaa accaacccgg cgaaatttgc atccgaggct
     5221 cccaaatcat gaaagggtat cttaatgacg cggcggctac gcagaggacg atcgacaagg
     5281 aagggtggct gcacaccggc gacattggct atgtcgacga cgacgaggag ctcttcattg
     5341 tcgataggtt gaaggagatc attaagtaca agggcttcca agtcgcccct gccgagctcg
     5401 aagccattct cattactcac cctaacattg ctgatgccgc tgttgtcccg atgaaagatg
     5461 aggcagcagg ggaagtgcca gtggcatttg tggtgacctc caatggatca gtcatcagtg
     5521 aggatgagat caagcagttc attagcaagc aggtggtgtt ctacaagcga atcaatcgag
     5581 tctttttcgt tgattcaatt cctaaagcac cctctgggaa gattttgagg aaggatttga
     5641 ggggaagatt ggcagctggt atacccaagt agactagtgg atcccccggg ctgcaggaat
     5701 tcgatatcaa gcttatcgat accgtcgacc tcgagtcatg taattagtta tgtcacgctt
     5761 acattcacgc cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga
     5821 agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt
     5881 tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac
     5941 cttgcttgag aaggttttgg gacgctcgaa ggctttaatt tgcggccggt acccaattcg
     6001 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     6061 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     6121 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     6181 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6241 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     6301 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6361 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6421 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6481 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6541 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6601 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
     6661 ccttacgcat ctgtgcggta tttcacaccg catatcgacc ggtcgaggag aacttctagt
     6721 atatctacat acctaatatt attgccttat taaaaatgga atcccaacaa ttacatcaaa
     6781 atccacattc tcttcaaaat caattgtcct gtacttcctt gttcatgtgt gttcaaaaac
     6841 gttatattta taggataatt atactctatt tctcaacaag taattggttg tttggccgag
     6901 cggtctaagg cgcctgattc aagaaatatc ttgaccgcag ttaactgtgg gaatactcag
     6961 gtatcgtaag atgcaagagt tcgaatctct tagcaaccat tatttttttc ctcaacataa
     7021 cgagaacaca caggggcgct atcgcacaga atcaaattcg atgactggaa attttttgtt
     7081 aatttcagag gtcgcctgac gcatatacct ttttcaactg aaaaattggg agaaaaagga
     7141 aaggtgagag cgccggaacc ggcttttcat atagaataga gaagcgttca tgactaaatg
     7201 cttgcatcac aatacttgaa gttgacaata ttatttaagg acctattgtt ttttccaata
     7261 ggtggttagc aatcgtctta ctttctaact tttcttacct tttacatttc agcaatatat
     7321 atatatatat ttcaaggata taccattcta atgtctgccc ctaagaagat cgtcgttttg
     7381 ccaggtgacc acgttggtca agaaatcaca gccgaagcca ttaaggttct taaagctatt
     7441 tctgatgttc gttccaatgt caagttcgat ttcgaaaatc atttaattgg tggtgctgct
     7501 atcgatgcta caggtgttcc acttccagat gaggcgctgg aagcctccaa gaaggctgat
     7561 gccgttttgt taggtgctgt gggtggtcct aaatggggta ccggtagtgt tagacctgaa
     7621 caaggtttac taaaaatccg taaagaactt caattgtacg ccaacttaag accatgtaac
     7681 tttgcatccg actctctttt agacttatct ccaatcaagc cacaatttgc taaaggtact
     7741 gacttcgttg ttgtcagaga attagtggga ggtatttact ttggtaagag aaaggaagac
     7801 gatggtgatg gtgtcgcttg ggatagtgaa caatacaccg ttccagaagt gcaaagaatc
     7861 acaagaatgg ccgctttcat ggccctacaa catgagccac cattgcctat ttggtccttg
     7921 gataaagcta atgttttggc ctcttcaaga ttatggagaa aaactgtgga ggaaaccatc
     7981 aagaacgaat tccctacatt gaaggttcaa catcaattga ttgattctgc cgccatgatc
     8041 ctagttaaga acccaaccca cctaaatggt attataatca ccagcaacat gtttggtgat
     8101 atcatctccg atgaagcctc cgttatccca ggttccttgg gtttgttgcc atctgcgtcc
     8161 ttggcctctt tgccagacaa gaacaccgca tttggtttgt acgaaccatg ccacggttct
     8221 gctccagatt tgccaaagaa taaggtcaac cctatcgcca ctatcttgtc tgctgcaatg
     8281 atgttgaaat tgtcattgaa cttgcctgaa gaaggtaagg ccattgaaga tgcagttaaa
     8341 aaggttttgg atgcaggtat cagaactggt gatttaggtg gttccaacag taccaccgaa
     8401 gtcggtgatg ctgtcgccga agaagttaag aaaatccttg cttaaaaaga ttctcttttt
     8461 ttatgatatt tgtacataaa ctttataaat gaaattcata atagaaacga cacgaaatta
     8521 caaaatggaa tatgttcata gggtagacga aactatatac gcaatctaca tacatttatc
     8581 aagaaggaga aaaaggagga tgtaaaggaa tacaggtaag caaattgata ctaatggctc
     8641 aacgtgataa ggaaaaagaa ttgcacttta acattaatat tgacaaggag gagggcacca
     8701 cacaaaaagt taggtgtaac agaaaatcat gaaactatga ttcctaattt atatattgga
     8761 ggattttctc taaaaaaaaa aaaatacaac aaataaaaaa cactcaatga cctgaccatt
     8821 tgatggagtt taagtcaata ccttcttgaa ccatttccca taatggtgaa agttccctca
     8881 agaattttac tctgtcagaa acggccttaa cgacgtagtc gatatggtgc actctcagta
     8941 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     9001 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     9061 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcga
//
