LOCUS       sequence-230405-       11290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express MS2-P65-HSF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11290)
  AUTHORS   Lu B, Klingbeil O, Tarumoto Y, Somerville TDD, Huang YH, Wei Y, Wai 
            DC, Low JKK, Milazzo JP, Wu XS, Cao Z, Yan X, Demerdash OE, Huang G,
            Mackay JP, Kinney JB, Shi J, Vakoc CR
  TITLE     A Transcription Factor Addiction in Leukemia Imposed by the MLL 
            Promoter Sequence.
  JOURNAL   Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 
            10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29.
  PUBMED    30503706
REFERENCE   2  (bases 1 to 11290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.10.015"; journalName: "Cancer Cell"; date: 
            "2018-12-10- 10"; volume: "34"; issue: "6"; pages: "970-981.e8"
COMMENT     Sequence Label: Lenti_MS2_P65_HSF1_2A_puro
FEATURES             Location/Qualifiers
     source          1..11290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..402
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             457..477
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             493..1035
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             1060..1431
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             1447..1500
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1501..2097
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     1994..2014
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2117..2705
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2170..2190)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2588..2599)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2708..2724)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2709..2725)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3230..3410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3436..3453)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3442..3666
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3712..4140
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3799..3818)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4009..4030
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4149..4169)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4154..4483
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4334..4469
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4396..4415
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4531..4578
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4597..4971
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5101..5222
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5138..5157)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5192..5211
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5271..5287)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5271..5287)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5284..5306)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5295..5311
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5319..5325,5326..5343,5344..5349))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5364..5385
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5502..5519)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5673..6261)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5753..5772)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6432..7223,7224..7292))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7055..7074
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7293..7397)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7472..7491)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7663..8042
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8044..8242
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8197..8217
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8260..8440
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8487..8612
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9105..9338
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9523..9567
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9865..9982
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        10112..11290
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          10343..11281
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     11238..11258
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 cgtacggcca ccatggcttc aaactttact cagttcgtgc tcgtggacaa tggtgggaca
       61 ggggatgtga cagtggctcc ttctaatttc gctaatgggg tggcagagtg gatcagctcc
      121 aactcacgga gccaggccta caaggtgaca tgcagcgtca ggcagtctag tgcccagaag
      181 agaaagtata ccatcaaggt ggaggtcccc aaagtggcta cccagacagt gggcggagtc
      241 gaactgcctg tcgccgcttg gaggtcctac ctgaacatgg agctcactat cccaattttc
      301 gctaccaatt ctgactgtga actcatcgtg aaggcaatgc aggggctcct caaagacggt
      361 aatcctatcc cttccgccat cgccgctaac tcaggtatct acagcgctgg aggaggtgga
      421 agcggaggag gaggaagcgg aggaggaggt agcggaccta agaaaaagag gaaggtggcg
      481 gccgctggat ccccttcagg gcagatcagc aaccaggccc tggctctggc ccctagctcc
      541 gctccagtgc tggcccagac tatggtgccc tctagtgcta tggtgcctct ggcccagcca
      601 cctgctccag cccctgtgct gaccccagga ccaccccagt cactgagcgc tccagtgccc
      661 aagtctacac aggccggcga ggggactctg agtgaagctc tgctgcacct gcagttcgac
      721 gctgatgagg acctgggagc tctgctgggg aacagcaccg atcccggagt gttcacagat
      781 ctggcctccg tggacaactc tgagtttcag cagctgctga atcagggcgt gtccatgtct
      841 catagtacag ccgaaccaat gctgatggag taccccgaag ccattacccg gctggtgacc
      901 ggcagccagc ggccccccga ccccgctcca actcccctgg gaaccagcgg cctgcctaat
      961 gggctgtccg gagatgaaga cttctcaagc atcgctgata tggactttag tgccctgctg
     1021 tcacagattt cctctagtgg gcagggagga ggtggaagcg gcttcagcgt ggacaccagt
     1081 gccctgctgg acctgttcag cccctcggtg accgtgcccg acatgagcct gcctgacctt
     1141 gacagcagcc tggccagtat ccaagagctc ctgtctcccc aggagccccc caggcctccc
     1201 gaggcagaga acagcagccc ggattcaggg aagcagctgg tgcactacac agcgcagccg
     1261 ctgttcctgc tggaccccgg ctccgtggac accgggagca acgacctgcc ggtgctgttt
     1321 gagctgggag agggctccta cttctccgaa ggggacggct tcgccgagga ccccaccatc
     1381 tccctgctga caggctcgga gcctcccaaa gccaaggacc ccactgtctc ctgtacaggc
     1441 agtggagagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctggccca
     1501 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     1561 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     1621 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     1681 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     1741 gtcgaagcgg gggcggtttt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     1801 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     1861 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     1921 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     1981 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     2041 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacga
     2101 tatcaagctt atcggtaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     2161 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     2221 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     2281 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     2341 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     2401 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     2461 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     2521 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     2581 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     2641 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     2701 cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa
     2761 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     2821 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     2881 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     2941 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     3001 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     3061 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     3121 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     3181 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     3241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     3301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     3361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
     3421 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     3481 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     3541 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     3601 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     3661 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     3721 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3781 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3841 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3901 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     3961 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4021 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4081 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4141 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4201 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     4261 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4321 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4381 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     4441 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     4501 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     4561 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
     4621 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     4681 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     4741 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     4801 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     4861 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     4921 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
     4981 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     5041 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     5101 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5161 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5221 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     5281 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     5341 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     5401 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     5461 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     5521 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     5581 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     5641 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5701 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5761 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5821 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5881 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5941 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6001 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6061 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     6121 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6181 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6241 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6301 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6361 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     6421 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     6481 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     6541 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     6601 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     6661 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     6721 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     6781 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     6841 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6901 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6961 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     7021 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     7081 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     7141 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     7201 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     7261 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     7321 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     7381 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     7441 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     7501 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     7561 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     7621 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
     7681 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     7741 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     7801 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     7861 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     7921 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     7981 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     8041 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     8101 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     8161 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     8221 ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag
     8281 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     8341 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     8401 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     8461 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     8521 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     8581 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     8641 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     8701 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     8761 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     8821 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     8881 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     8941 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     9001 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     9061 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     9121 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     9181 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     9241 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     9301 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     9361 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     9421 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     9481 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     9541 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     9601 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     9661 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     9721 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     9781 gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag
     9841 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     9901 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     9961 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt
    10021 aattagctag ctgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg
    10081 gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg
    10141 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta
    10201 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
    10261 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
    10321 cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt
    10381 tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct
    10441 tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc
    10501 cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat
    10561 ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt
    10621 ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga
    10681 tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca
    10741 gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta
    10801 gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc
    10861 ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc
    10921 cggccctgct gcagggagct caaaatgaag gacgcggcgc tcgggagagc gggcgggtga
    10981 gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac
    11041 ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc
    11101 tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac
    11161 tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt
    11221 tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca
    11281 ggtgtcgtga
//
