LOCUS       sequence-230409-        9226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express human wild type ZFP64 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9226)
  AUTHORS   Lu B, Klingbeil O, Tarumoto Y, Somerville TDD, Huang YH, Wei Y, Wai 
            DC, Low JKK, Milazzo JP, Wu XS, Cao Z, Yan X, Demerdash OE, Huang G,
            Mackay JP, Kinney JB, Shi J, Vakoc CR
  TITLE     A Transcription Factor Addiction in Leukemia Imposed by the MLL 
            Promoter Sequence.
  JOURNAL   Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 
            10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29.
  PUBMED    30503706
REFERENCE   2  (bases 1 to 9226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.10.015"; journalName: "Cancer Cell"; date: 
            "2018-12-10- 10"; volume: "34"; issue: "6"; pages: "970-981.e8"
COMMENT     Sequence Label: LentiV_Neo_WT_ZFP64
FEATURES             Location/Qualifiers
     source          1..9226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2078..2134
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2153..2947
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2207..2226)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2817..2836
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    2963..3551
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3016..3036)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3434..3445)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3623..3856
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3934..4055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3971..3990)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4025..4044
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4095..4230
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4250..4269)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4251..4269)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4279..4296)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4279..4295)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4288..4310)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4437..4892
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4524..4543)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4734..4755
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4918..5022
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5023..5091,5092..5883)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5241..5260)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6054..6642
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6543..6562
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6796..6813
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6930..6951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6966..6971,6972..6989,6990..6996)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7004..7020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7009..7031
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7028..7044
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7028..7044
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7063..7083
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7065..7083
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7111..7337
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7338..7518
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7565..7690
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8183..8416
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8601..8645
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8829..8946
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9015..9226
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 gtgtcgtgac gcgggatccg ccaccatgaa cgcgagcagc gagggcgaga gcttcgcggg
       61 ctcggtgcaa attccaggtg gcacaacggt gctggtggag ctgactcccg acatccatat
      121 ctgcggcatc tgcaagcagc agtttaacaa cctggatgcc tttgtagctc acaagcaaag
      181 tggctgccag ctgacaggca catccgcagc agcccccagc acggtccagt ttgtatcgga
      241 ggaaacagtg cctgccaccc agactcagac caccaccaga accatcacct cggagaccca
      301 gacaatcaca gtttcagctc cagaatttgt ttttgaacat ggctatcaaa cttacctgcc
      361 cacggaaagt aatgaaaacc agacagccac tgtcatctct ctccctgcca agtcacgcac
      421 caaaaagccc acaacaccac ctgctcagaa aaggcttaac tgttgctatc caggttgcca
      481 attcaagact gcttatggca tgaaggacat ggagcggcat ttaaaaattc acacgggaga
      541 caaaccccat aagtgtgaag tctgtggcaa gtgctttagc cggaaagaca agctgaaaac
      601 tcacatgcgg tgccacacgg gcgtgaagcc ctacaagtgt aagacgtgtg actacgccgc
      661 tgccgacagc agcagcctca acaagcacct gaggatccac tcggacgagc ggcccttcaa
      721 atgccagatc tgcccctacg ccagccgcaa ctccagccag ctcactgtcc acctgcgatc
      781 ccacacgggg gacgccccct tccagtgctg gctctgtagc gccaagttca aaatcagctc
      841 ggacttgaaa aggcacatgc gggtgcactc gggggagaag cctttcaagt gcgagttctg
      901 caatgtccgc tgcaccatga aggggaacct caagtcgcac atccgtatca agcacagcgg
      961 gaataacttc aagtgtcctc attgcgactt cctgggtgac agcaaagcca ccctccggaa
     1021 gcacagccgc gtgcaccagt cggagcatcc tgagaagtgc tcggaatgca gctactcctg
     1081 ctccagcaag gccgccctgc gcatccacga gcgtatccac tgcaccgacc gccctttcaa
     1141 gtgcaactac tgcagcttcg acaccaaaca gcccagcaac ctgagcaagc acatgaagaa
     1201 gttccacggg gacatggtta agactgaggc tctagagagg aaggacaccg gcaggcagag
     1261 cagccggcag gtggccaagc tggatgccaa gaagagtttc cactgcgata tatgcgatgc
     1321 ctccttcatg cgggaggact cgctccgcag ccacaagaga cagcacagtg agtacagtga
     1381 gagtaagaac tcggacgtga ccgttctcca gtttcagatc gaccccagca agcagcccgc
     1441 cacgcccctc actgtgggac acctccaggt gcccctccag cccagccaag tgccccagtt
     1501 cagcgaggga agagtcaaaa tcatcgttgg gcatcaggtg ccccaggcga acaccatcgt
     1561 ccaggctgcc gctgctgcag tgaacatcgt cccgcctgcc ttggtggccc agaacccaga
     1621 ggaactccca gggaacagcc ggctgcagat cctgcgccag gtcagtctga tcgccccccc
     1681 tcagtcctcg cggtgtccga gcgaggcggg cgcaatgacc cagccggctg tcctgctgac
     1741 cacccacgag cagacggacg gagccactct gcaccagact ctcatcccca cggcctcagg
     1801 tggcccccag gaaggctctg gcaatcaaac tttcattacc agttcgggta ttacttgcac
     1861 tgactttgaa ggcctaaacg ccttgattca ggaggggaca gcagaagtga cagtggtgag
     1921 cgatggaggc cagaacatcg cagtggccac cacagcgcca ccggtcttct cctcctcttc
     1981 ccagcaagaa ctacccaagc agacctactc catcattcaa ggggcagccc atccagcttt
     2041 gctctgtccc gccgactcca ttccagatgg cagcggcgcc accaacttca gcctgctgaa
     2101 gcaggccggc gacgtggagg agaaccccgg ccccatgacc gagtacaagc ccatgattga
     2161 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     2221 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     2281 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     2341 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     2401 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     2461 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     2521 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     2581 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     2641 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     2701 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     2761 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     2821 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     2881 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     2941 cttctgaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     3001 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     3061 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     3121 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     3181 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     3241 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     3301 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     3361 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     3421 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     3481 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     3541 cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     3601 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     3661 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     3721 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     3781 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     3841 tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa
     3901 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     3961 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4021 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     4081 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4141 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     4201 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     4261 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     4321 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     4381 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     4441 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4501 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4561 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4621 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4681 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4741 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4801 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4861 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     4921 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4981 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5041 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5101 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5161 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5221 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     5281 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5341 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5401 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5461 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5521 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     5581 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5641 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5701 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5761 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5821 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5881 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5941 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6001 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6061 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6121 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6181 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6241 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6301 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6361 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6421 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6481 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6541 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6601 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6661 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6721 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6781 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     6841 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     6901 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     6961 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     7021 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     7081 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     7141 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     7201 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     7261 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     7321 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7381 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7441 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7501 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     7561 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     7621 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     7681 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     7741 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     7801 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     7861 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     7921 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     7981 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     8041 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     8101 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     8161 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     8221 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     8281 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     8341 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     8401 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     8461 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     8521 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     8581 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     8641 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     8701 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     8761 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca
     8821 gaccacaatt ttaaaagaaa aggggggatt gggaggtaca gtgcagggga aagaatagta
     8881 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     8941 aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct cgagtggctc
     9001 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     9061 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     9121 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     9181 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacag
//
